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Volume 47, Issue 5
Displaying 1-33 of 33 articles from this issue
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Tadashi KOBAYASHIArticle type: Article
1973 Volume 47 Issue 5 Pages 187-200
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSRecent progress in the thermal and photochemical isomerization of vitamin D group is reviewed. In the field of thermal isomerization, the reversible reaction between vitamin D and previtamin D and the irreversible reaction of vitamin D into pyro- and isopyrovitamin D via previtamin D are discussed. In the field of photochemical isomerization, the interconversions of provitamin D, previtamin D, lumisterol and tachysterol are discussed and then the over-irradiation products of vitamin D group are described.View full abstractDownload PDF (1028K) -
Denichi MIURAArticle type: Article
1973 Volume 47 Issue 5 Pages 201-209
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSWashed cells of Staphylococcus aureus FDA 209P, when incubated with thiamine (B_1) in the medium having low ionic strength, adsorbed the vitamin nonenzymatically. The amount of B_1 adsorbed could not be ignored when uptake reaction was considered. Lower temperature and lower ionic strength favoured the adsorption. The ^<14>C-thiamine adsorbed by the cell, when added to the new medium containing unlabeled B_1,was exchanged immediately with the outer B_1. In its adsorption equilibrium, which was attained in approximately 40 seconds, the concentration difference between adsorbed and not adsorbed B_1 was several hundred. Dissociation constant of the adsorption reaction was 2.0×10^<-5>M. Boiled cells adsorbed B_1 to about the same amount as unboiled cells or even more than them. Between pH 4 and 9,the cells adsorbed more B_1 when more acidic medium was used. Adsorption of free B_1 was suggested as ionic one. TMP (approximately 1/10 of B_1) and TDP (approximately 1/20 of B_1) were also adsorbed, but some different mechanism was presumed to exist in the adsorption of TDP.View full abstractDownload PDF (648K) -
Takahiro NISHIMUNE, Denichi MIURA, Ryoji HAYASHIArticle type: Article
1973 Volume 47 Issue 5 Pages 211-219
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSWashed cells of Staphylococcus aureus FDA 209P accumulated thiamine (B_1) in the presence of glucose and high concentration of buffer (more than 0.1 M) at 37℃. The process of accumulation was clearly distinguished from the adsorption of B_1 by (1) heat instability, (2) optimum pH (8.2 or more alkaline), (3) accumulation of B_1 phosphates, (4) accumulation in the presence of high ionic strength, (5) optimum temperature (42℃ for the initial rate), and (6) Km (1.3×10^<-6>M). The uptake activity was repressed by high concentration of B_1 in the growth medium. Pyrithiamine competed the uptake of free B_1 and free B_1 competed the accumulation of pyrithiamine. Pyrithiamine was taken up and phosphorylated in the cell. TDP was also taken up by the cell and accumulated as B_1 phosphates, which could not be accounted for by the mechanisms posturating the hydrolysis of TDP to free B_1 prior to the penetration into bacterial membrane, followed by phosphorylation of free B_1 in the cell.View full abstractDownload PDF (719K) -
Takahiro NISHIMUNEArticle type: Article
1973 Volume 47 Issue 5 Pages 221-227
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSIn the accumulation process of thiamine by Staphylococcus aureus FDA 209P cells, phosphorylation of thiamine (B_1) could be separated and distinguished from uptake process. The cell used in this experiment was presaturated with B_1 phosphates as follows : The cell, which had been shaken at 37℃ with free B_1 for 180 minutes in the presence of glucose, was collected by centrifuge, resuspended in three times the volume of 0.9% NaCl and shaken at 37℃ for 60 minutes. Normally more than 95% of the accumulated B_1 in the cell thus obtained was phosphorylated forms. The ^<14>C-B_1 taken up by the B_1 phosphates-presaturated cell accumulated as free B_1 during 3 to 5 minutes of incubation, but in longer period of incubation ^<14>C-B_1 phosphates started to accumulate. The free B_1 accumulation reaction depended on the presence of glucose and temperature. The Km for free B_1 accumulation was 3.9×10^<-7> M. Small amount of B_1 phosphates accumulated in the cell leaked out in a process which needed glucose and an incubation at 37℃.View full abstractDownload PDF (539K) -
Choten INAGAKI, Chieko OKITA, Nobuhiko ARAKAWAArticle type: Article
1973 Volume 47 Issue 5 Pages 229-232
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSThe present investigation with the purpose to study the gas-liquid chromatographic determination on fatty acid esters of L-ascorbic acid (6-mono-Pro-AsA, 6-mono-But-AsA, 6-mono-Oct-AsA, 6-mono-Dec-AsA, 6-mono-Lau-AsA, 6-mono-Pal-AsA, 2-mono-Lau-AsA, 5,6-di-Pro-AsA and 2,6-di-Lau-AsA) was carried out. After the formation of the trimethylchlorosilane derivative in pyridine containing hexamethyl-disilazane and trimethylchlorosilane, each sample was subjected to gas chromatography with glass column (chromosorb-AW cotaining 1% silicone OV-17). Three kinds of n-paraffins (C_<24>, C_<28> and C_<32>) complied with the carbon number of fatty acid ester of L-ascorbic acid were used as the internalstandard (for example, C_<32>H_<66> to 6-mono-Lau-AsA), and 6-monoesters and 5,6-diester including short carbon chain fatty acid ester were determined in high accuracy.View full abstractDownload PDF (315K) -
Teijiro MIYAMOTO, Kiku MURATA, Makiko KAWAMURAArticle type: Article
1973 Volume 47 Issue 5 Pages 233-237
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSSome kinds of miso, soy-sauce, natto and their materials were analyzed for these folate values by microbiological assay with Streptococcus faecalis and Lactobacillus casei as the test organism. It was observed that folate activity was decreased during soy-sauce brewing, and folate activities measured with L.casei and S.faecalis were not so different. Folate values of twenty-four kinds of vegetables were determined using L.casei. The values of folate extracted in the presence of ascorbic acid were generally higher than those obtained without ascorbate added. These data were compared with some published values, and discussed. Folate intakes of three college women were calculated according to their menus. Ingestions of free folate and total folate were 135〜189 mcg per day and 377〜807 mcg per day respectively.View full abstractDownload PDF (450K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 239-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (154K) -
[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 239-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (154K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 239-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (154K) -
D.S. Murdock, C.J. GublerArticle type: Article
1973 Volume 47 Issue 5 Pages 240-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (189K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 240-241
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (206K) -
[in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 243-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (160K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 243-244
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (285K) -
[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 244-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (172K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 244-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (172K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 244-245
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (303K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 245-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (184K) -
[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 245-246
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (195K) -
[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 247-248
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (295K) -
[in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 248-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (182K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 248-249
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (302K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 249-250
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (307K) -
[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 250-251
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (262K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 251-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (124K) -
[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 253-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (161K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 253-254
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (289K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 254-255
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 255-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
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[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 255-256
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (318K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 256-257
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (308K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 257-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (175K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 257-258
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (266K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 5 Pages 258-
Published: May 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (139K)
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