VITAMINS
Online ISSN : 2424-080X
Print ISSN : 0006-386X
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Volume 40, Issue 1
Displaying 1-16 of 16 articles from this issue
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  • STUDIES ON THE EXISTENCE OF THE INTERMEDIATE BETWEEN PANTOTHENIC ACID AND COENZYME A IN RAT LIVER AND ITS BIOLOGICAL MEANING, PARTICULARLY IN THE RELATION WITH ACYL CARRIER PROTEIN
    Tsuneo NAKAMURA
    Article type: Article
    1969 Volume 40 Issue 1 Pages 1-16
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_1
    JOURNAL FREE ACCESS
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    The conventional determination methods for pantothenic acid and CoA were examined and some amendment points were discussed. By using these modified determination methods the changes in blood pantothenic acid level of children and in pantothenic acid and CoA contents of rat tissues according to growth were analysed. In comparing with CoA contents determined by sulfanylamide acetylation method and sorbyl-CoA formation method, it was presumed that rat liver contained a relatively large amount of phosphopantetheine and dephospho-CoA besides CoA as a bound pantothenic acid. In order to confirm this point the in vitro biosynthesis of CoA from phosphopantetheine or decomposition of phosphopantetheine from CoA was investigated. The existance of phosphopantetheine and dephospho-CoA in rat liver as a bound pantothenic acid was confirmed from these observations. The amount of this intermediate between pantothenic acid and CoA in rat liver was definitely influenced by pantothenic acid content in diet, adrenalectomy and administration of glucocorticoid, antibiotics, anticancer drugs or large doses of thiamine derivatives. Then, this intermediate was accurately identified as phosphopantetheine after isolation and confirmation by colum- and paper-chromatographies. The amount of phosphopantetheine that was determined by both above mentioned indirect and direct methods was quite equal. It was recognized that this phosphopantetheine was not produced by the decomposition of CoA in rat liver during preparation of samples with treatment of hot water extraction. The relation between this intermediate and acyl carrier protein found in Escherichia coli or fatty acid synthetic multi-enzyme complex were observed in order to clarify the biological meaning of phosphopantetheine in rat liver.
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  • STUDIES ON THE DETERMINATION OF VITAMINN C BY HYDRAZINE METHOD AND THE DETERMINATION OF TRUE VITAMIN C BY THIN-LAYER CHROMATOGRAPHY
    Akiji FUJITA, Fukuko HIROSE, Yoshiko UCHIYAMA
    Article type: Article
    1969 Volume 40 Issue 1 Pages 17-26
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_17
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    (1) The determination of vitamin C by 2,4-dinitrophenylhydrazine(Hyd) method is influenced by the presence of various compounds, for instance, aromatic amino acids, keto acids, sugars, uronic acids, reductone and tannic acid, especially when oxidized with bromine rather than 2,6-dichlorophenolindophenol(Ind) . (2) When the reaction products of Hyd method of various animal and vegetable tissues and their preparations were subjected to thin-layer chromatography(TLC) many colored spots appeared in the chromatogram in addition to the one corresponding to ascorbic acid(AsA). However, the absorption spectra of the colored solutions of many tissues oxidized with Ind corrected with the blank test agreed pretty well with that of pure AsA, when the tissues contained much AsA, but not in the case of those containing little AsA. (3) The determinathion of real total vitamin C by TLC using the colored solution of the Hyd method oxidized with Ind is described in detail. The colored solution is extracted with ethylacetate and subjected to TLC using silica gel G as the adsorbent and toluene・acetone・5% acetic acid as the developing solvent. The fraction correponding to AsA is dissolved in sulfuric acid and determined photometrically at 530 mμ. (4) Comparison of the Hyd values of many fresh tissues oxidized with Ind or bromine with those of TLC showed that those oxidized with Ind and the real vitamin C values by TLC did not differ markedly but those oxidized with bromine were generally much greater than TLC values. (5) Many foodstuffs of animal and vegetable origins showed remarkable AsA values in the Hyd method, whereas the TLC values were negligible, for example, black tea, coffee, cocoa, soy sauce, fruit juice, and bean paste. The TLC method is proved to be useful for testing and determinining the existence of true vitamin C in tissues and products.
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  • EFFECT OF ADMINISTRATION OF THIAMINE, RIBOFLAVIN, ASCORBIC ACID AND VITAMIN A TO STUDENTS ON THEIR PANTOTHENIC ACID CONTENT IN SERUM AND URINE
    Tatsuo KOYANAGI, Shin-ichi HAREYAMA, Ryosuke KIKUCHI, Teru TAKANOHASHI ...
    Article type: Article
    1969 Volume 40 Issue 1 Pages 27-31
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_27
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    After dosing of thiamine, riboflavin, pyridoxine, ascorbic acid, nicotinic acid, vitamin A and B_<12> together to 20 students the concentration of pantothenic acid in serum showed an increase in 7 students and a decrease in 3 students. By examination of effect of separate administration of thiamine, riboflavin, ascorbic acid or vitamin A the concentration of pantothenic acid was found to increase by thiamine and in lesser extent by riboflavin. Excretion of pantothenic acid in 24 hours urine showed a same trend as the pantothenic acid concentration of serum, i. e. , dosing of thiamine or in lesser degree of riboflavin increased the excretion of pantothenic acid .
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  • STUDIES ON LIPOPEROXIDES IN SERUM OF NORMAL PREGNANT WOMEN
    Minoru ISHIHARA, Ikuo NISHIGAKI, Kunio YAGI
    Article type: Article
    1969 Volume 40 Issue 1 Pages 32-37
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_32
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    The amount of lipoperoxides in serum of normal pregnant women was examined by TBA metnod ot Yagi, Nishigaki and Ohama. In the second and the third trimester, higher level of TBA value than that of non-pregnant women as well as than that of the pregnant women in the first trimester was observed. TBA values of the lipids extracted from the sera with chloroform・methanol mixture (2 : 1) showed the same tendency. By separating serum lipids using thin layer chromatography, triglyceride was found to show the highest TBA value among the lipids separated.
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  • STUDIES OF THE METABOLISM OF NICOTINAMIDE ADENINE DINUCLEOTIDE
    Tsuguji NEGISHI, Arata ICHIYAMA
    Article type: Article
    1969 Volume 40 Issue 1 Pages 38-45
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_38
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    The metabolism of NAD was investigated with mice following the administration of NAD- (nicotinamide-7^<14>C) and NAD-(adenosine-U^<l4>C). When NAD was given intravenously, it was not utilized directly as a coenzyme, and was degraded immediately to nicotinamide and presumably to adenosine diphosphate ribose. The ADP-ribose moiety was converted rapidly to ADP, AMP, ATP and two unknown metabolites. Even 20 seconds after the injection of NAD-(nicotinamide-7^<14>C) , more than 90% of radioactivity in the blood was shown to be nicotinamide. The nicotinamide disappeared rapidly from the blood and was incorporated into various tissues and organs. In addition, a portion of nicotinamide was excreted into the gastrointestinal tract. The radioactive material was reabsorbed and reutilized as a precursor to NAD in various tissues. When NAD-(nicotinamide-7^<14>C) was given orally, it was shown to be degraded to nicotinamide. The latter compound was absorbed efficiently and distributed in every tissue and organ. Practically no radioactivity was found in the feces. About 20% of total radioactivity was expired as ^<14>C0_2 Whithin 8 hours. Probably, the intestinal bacteria were responsible for this complete degradation of the nicotinamide.
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  • INFLUENCE OF IRON IN THE EFFECT OF CADMIUM UPON THE GUANOSINE TRIPHOSPHATE-DEPENDENT FLAVIN PHOSPHATE SYNTHETASE ACTIVITY OF PHIZOPUS JAVANICUS
    Sei TACHIBANA, Yosio TAKAI, Jiuiti SIODE
    Article type: Article
    1969 Volume 40 Issue 1 Pages 46-49
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_46
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    It has been previously reported that Cd^<2+> and Fe^<3+> have remarkably opposite effects on riboflavin cyclic 2', 5'-monophosphate(FMN') production^<1)2)3)> by theGTP-dependent enzyme^<4)> of Rhizopus oryzae or Rh. javanicus as well as on their growth and on their acid production. When Fe^<2+> was added with Cd^<2+> in the culture medium of Rh. javanicus, these effects of Cd^<2+> were depressed. But when Zn^<2+> or Cu^<2+> was added with Cd^<2+> in the culture medium of the mold, effect of Zn^<2+> or Cu^<2+> seemed to be stimulated by Cd^<2+>.
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  • COMPARATIVE STUDIES OF THE METABOLISM OF RIBOFLAVIN OF ORDER MUCOFALES : (I) FORMATIONS OF RIBOFLAVINYLGLUCOSIDE WITH MUCOR AND OF A NEW RIBOFLAVIN DIPHOSPHATE WITH RHIZOPUS
    Sei TACHIBANA
    Article type: Article
    1969 Volume 40 Issue 1 Pages 50-52
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_50
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    It was found that riboflavinylglucoside and some riboflavinyloligosaccharides were formed by Mucor javanicus, M. racemosus and M. rouxianus. A new riboflavin diphosphate was discovered using Rhizopus javanicus. It was suggested that the comparative biochemistry of Mucor and Rhizopus might be useful for the investigation of the biological significances of these glycosides and phosphates of riboflavin.
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 1 Pages 53-
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_53_1
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 1 Pages 53-
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_53_2
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 1 Pages 53-
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_53_3
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 1 Pages 54-
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_54_1
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  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 1 Pages 54-
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_54_2
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 1 Pages 54-55
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_54_3
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 1 Pages 55-
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_55
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 1 Pages 56-
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_56_1
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 1 Pages 56-
    Published: July 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.1_56_3
    JOURNAL FREE ACCESS
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