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Volume 56, Issue 5-6
Displaying 1-19 of 19 articles from this issue
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Kazuya UCHIDA, Kazuko YAMADA, Hrroshi KUMAOKAArticle type: Article
1982 Volume 56 Issue 5-6 Pages 235-240
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSIt has been known that formate is incorporated into the pyrimidine moiety of thiamin with only small dilution of specific activity by some microorganisms. However, some disagreements have arisen regarding the specific carbons of the pyrimidine that are derived from formate. David et al. reported that formate is incorporated only into C-4 of the pyrimidine in Saccharomyces cerevisiae, whereas Johnson et al. reported the methyl group attached to C-2 is derived from formate. The present investigations were carried out to determine which pyrimidine carbon atoms are labeled when S. cerevisiae is grown in the presence of [^<14>C] formate. The results of degradative reactions different from the methods by David et al. and Johnson et al. show that formate is incorporated into C-4 of the pyrimidine in S. cerevisiae.View full abstractDownload PDF (549K) -
Yasuko INOUE, Noriko IWATANI, Masahiro OHSUGIArticle type: Article
1982 Volume 56 Issue 5-6 Pages 241-247
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSThe accumulation of biotin from linoleic acid (LeA) by a strain (P-21-6) of bacterium isolated from soil is described. The bacterium was grown at 28℃, for 3 days, with continuous shaking in a test tube containing 4 ml of a 3 % Peptone medium. After one day growth, each of fatty acids, amino acids and sulfur compounds was added to the medium. LeA was a most effective of various fatty acids on the accumulation of biotin, and Cys, Cys and FeSO_4 were also effective. The organism accumulated biotin in amount of 500 ng per ml of medium from LeA (250 μg/ml) in the presence of Cys (350 μg/ml). In addition, aeration was effective on the accumulation of extracellular biotin. Intracellular and extracellular biotin-vitamers were clarified as a biotin and biotin plus desthiobiotin, respectively. Through the results of taxonomical characteristics, strain P-21,-6 was considered to be a Bacillus sp..View full abstractDownload PDF (647K) -
Shinhachiro NOMI, Tsuguo TANAKA, Hisakazu YAMAGISHI, Junsuke SHIBATA, ...Article type: Article
1982 Volume 56 Issue 5-6 Pages 249-254
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSActivation of cell mediated immunity of Balb/c mice was achieved by treatment with retinylacetate (A-acetate) in vitro and in vivo. In vitro, splenic cells from Balb/c mice were cultured in a microtestplate and A-acetate or A-acetate plus PHA-P was added to culture medium. After 3 days incubation ^3H-TdR incorporation to the cells was measured. Low concentration of A-acetate (10^<-8>〜10^<-7>M) induced blastoid transformation of splenic lymphocytes, on the other hand, higher concentration of A-acetate (10^<-5>〜10^<-4>M) supressed PHA induced blastoid transformation. In vivo, after 1×10^6 tumor cells were inoculated into Balb/c heteromice and nudemice, the injection of 1 mg A-acetate retarded tumor outgrowth and a combination with weekly injection of 1 mg BCG was more effective for inhibition of tumor growth in heteromice, but was not effective in nudemice. These results suggest that one of the therapeutic effects of A-acetate on tumor growth was considered to be the activation of cell mediated immunity. Furthermore, BCG is considered to generate the host antitumor immunity, thus, the combined administration of A-acetate with BCG resulted in more effective for tumor outgrowth than single administration of A-acetate.View full abstractDownload PDF (532K) -
Nobuhiko ARAKAWA, Megumi OTSUKA, Tadao KURATAArticle type: Article
1982 Volume 56 Issue 5-6 Pages 255-262
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (748K) -
Article type: Appendix
1982 Volume 56 Issue 5-6 Pages 263-265
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (204K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1982 Volume 56 Issue 5-6 Pages 267-
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (157K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1982 Volume 56 Issue 5-6 Pages 267-268
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (283K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1982 Volume 56 Issue 5-6 Pages 268-
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (178K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1982 Volume 56 Issue 5-6 Pages 268-269
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (300K) -
[in Japanese], [in Japanese]Article type: Article
1982 Volume 56 Issue 5-6 Pages 269-
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (175K) -
[in Japanese]Article type: Article
1982 Volume 56 Issue 5-6 Pages 269-270
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (312K) -
[in Japanese], [in Japanese]Article type: Article
1982 Volume 56 Issue 5-6 Pages 270-271
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (279K) -
[in Japanese], [in Japanese]Article type: Article
1982 Volume 56 Issue 5-6 Pages 271-
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (141K) -
[in Japanese]Article type: Article
1982 Volume 56 Issue 5-6 Pages 273-274
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (274K) -
[in Japanese]Article type: Article
1982 Volume 56 Issue 5-6 Pages 274-275
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (318K) -
[in Japanese]Article type: Article
1982 Volume 56 Issue 5-6 Pages 275-
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (174K) -
[in Japanese], [in Japanese]Article type: Article
1982 Volume 56 Issue 5-6 Pages 275-278
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (463K) -
[in Japanese]Article type: Article
1982 Volume 56 Issue 5-6 Pages 279-280
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (218K) -
Article type: Cover
1982 Volume 56 Issue 5-6 Pages Cover2-
Published: June 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (196K)
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