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Volume 30 (1964)
- Issue 6 Pages 417-
- Issue 5 Pages 341-
- Issue 4 Pages 271-
- Issue 3 Pages 195-
- Issue 2 Pages 95-
- Issue 1 Pages 1-
Volume 30, Issue 6
Displaying 1-11 of 11 articles from this issue
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Makoto INABAArticle type: Article
1964 Volume 30 Issue 6 Pages 417-427
Published: December 25, 1964
Released on J-STAGE: February 07, 2018
JOURNAL FREE ACCESSJTC-3 cells were grown in YLE-media containing thiamine and its derivatives such as TPD, TATD, BTMP and CAT at high concentrations equivalent to 10 to 50 μg per ml of thiamine hydrochloride. The long-term tissue culture was performed in the case with thiamine and TPD. The effects of these compounds on the cell growth and the susceptibility of the cell to Poliovirus Type I were studied. As a result, it was recognized that TPD and TATD at high concentrations over 20 μg per ml had remarkable inhibitory effects on the cell growth, especially in the former, however, not so significant of both BTMP and CAT. On the other hand, thiamine showed no such effect on the cell growth, even after the longterm cultivation. The viral growth was slightly suppressed by the addition of both TPD and TATD. It would be realized that this suppression might be attributed to the reduced activity of the cells by these compounds.View full abstractDownload PDF (1439K) -
Makoto INABAArticle type: Article
1964 Volume 30 Issue 6 Pages 428-430
Published: December 25, 1964
Released on J-STAGE: February 07, 2018
JOURNAL FREE ACCESSJTC-3 cells were grown in YLE-media containing thiamine disulfide (TDS) and O-benzoylthiamine disulfide (BTDS) at high concentrations equivalent to 20 to 50 μg per ml of thiamine hydrochloride. The effects of these compounds on the cell growth and the susceptibility of the cell to Poliovirus Type I were studied. As a result, it was recognized that BTDS at high concentration as 50 μg per ml had slight inhibitory effect on the cell growth. On the other hand, TDS showed no such effect on the cell growth. On the viral growth both TDS and BTDS had no effect.View full abstractDownload PDF (395K) -
Toshio KURODAArticle type: Article
1964 Volume 30 Issue 6 Pages 431-435
Published: December 25, 1964
Released on J-STAGE: February 07, 2018
JOURNAL FREE ACCESSPyridoxine disulfide was synthesized by heating pyridoxine thiosulfate in acid solution containing thiourea, iodide or thiocyanate. It was also prepared either by acid decomposition of isopropylidene pyridoxine disulfide, obtained from isopropylidene pyridoxine chloride and Na_2S_2,or by alkali decomposition of pyridoxine thiocyanate.View full abstractDownload PDF (550K) -
Toshio KURODA, Masuko MASAKIArticle type: Article
1964 Volume 30 Issue 6 Pages 436-440
Published: December 25, 1964
Released on J-STAGE: February 07, 2018
JOURNAL FREE ACCESSPyridoxamine disulfide was synthesized by decomposition of Bunte salt, obtained by the reaction of pyridoxamine bromide and sodium thiosulfate. Bis-4-pyridoxyldisulfide and bis-4-pyridoxylphosphate disulfide was also obtained by the reaction of pyridoxal or its 5-phosphate with NH_4SH. These compounds gave 4-pyridoxylmercaptan with diluted HCl at 25℃. The latter was easily oxidized to bis-4-pyridoxyldisulfide with iodide.View full abstractDownload PDF (676K) -
Seizo YAMASHITAArticle type: Article
1964 Volume 30 Issue 6 Pages 441-445
Published: December 25, 1964
Released on J-STAGE: February 07, 2018
JOURNAL FREE ACCESSExperiments were carried out concerning the difference between neuroparalytic and non-paralytic rats of thiamine deficiency. In the neuroparalytic rat the following characteristics were observed. (1) Electrocardiographic change, heart weight increase, decrease of myocardial thiamine content were comparatively light. (2) Serum potassium content was low. (3) Serum cholesterol content was high. There were no difference in hemoglobin, hematocrit and serum protein between paralytic and non-paralytic rats.View full abstractDownload PDF (631K) -
Yukio SHIGETA, Mitsuru HOSHI, Motoaki SHICHIRI, Kiichi OJI, Mataemon T ...Article type: Article
1964 Volume 30 Issue 6 Pages 446-448
Published: December 25, 1964
Released on J-STAGE: February 07, 2018
JOURNAL FREE ACCESSIt is well known that peripheral neuropathy is one of the common complications of diabetes mellitus. In such patients the increased threshold of vibratory perception in fingers and toes which estimated by biothesiometer as same as other abnormal signs and subjective symptoms were frequently observed. Pantethine, which is a derivative of pantothenic acid with β-mercaptoethylamine, was used for the treatment of diabetic neuropathy. The administration of this compound for 20 to 70 days produced an improvement in vibratory perception as well as patellar tendon reflex and subjective symptoms such as pain and parasthesia of extremities. The increasing ratio of urinary excretion of pyruvate following fructose loading was also decreased by such a treatment in these patients.View full abstractDownload PDF (370K) -
Kichitaro FUKUDAArticle type: Article
1964 Volume 30 Issue 6 Pages 449-460
Published: December 25, 1964
Released on J-STAGE: February 07, 2018
JOURNAL FREE ACCESSThe results obtained in the mixed culture of Lactobacillus acidophilus and Bacillus aneurinolyticus, indicate that the culture of the former has almost no influence on the growth and the thiaminase activity of the latter. In the mixed culture of B. aneurinolyticus and Streptococcus faecalis, the latter inhibits the growth of the former at pH 3.8〜4.2. However, no remarkable interactions are observed between these two organisms cultivated in phosphatebuffered media (pH 6.0〜6.8), and the thiaminase activity of B. aneurinolyticus is not suppressed by the presence of S. faecalis. In the mixed culture of B. thiaminolyticus and S. faecalis, the latter inhibits both of the growth and the thiaminase activity of the former at pH 4.0〜5.0. S. faecalis, grown in phosphate-buffered media (pH 5.8〜6.0) has, on the contrary, no effect on the growth of B. thiaminolyticus.View full abstractDownload PDF (1021K) -
Chisae YAMADAArticle type: Article
1964 Volume 30 Issue 6 Pages 461-465
Published: December 25, 1964
Released on J-STAGE: February 07, 2018
JOURNAL FREE ACCESSAcceleration of fermentation system seems to be one of the probable causes for growth-inhibition of Saccharomyces carlsbergensis at the presence of thiamine. To clarify this, fermentation-stimulating activity of modified thiamine compounds, of which growth-inhibitory effects on the yeast were studied previously (Vitamins 30,358,1964), was determined after Atkin et al.. Among disulfide-type thiamines TPD, the most effective growth-inhibitor, stimulated CO_2 evolution as much as thiamine, while BTDS with no growth-inhibitory effect showed no stimulation. Among S-acyl-type thiamines BTMP showed rather weaker stimulation compared to thiamine but DBT was weaker than BTMP in stimulating fermentation. Some of the antithiamines and pyrimidine or thiazole moieties of thiamine were all ineffective on fermentation. With exception of antithiamines, the more inhibitory on the growth of Sacch. carlsbergensis, the more stimulative on fermentation. Consumption of glucose and production of aloohol per dry weight of cells were also stimulated at the presence of thiamine.View full abstractDownload PDF (663K) -
Koji HOSHINO, Masami YAGYUArticle type: Article
1964 Volume 30 Issue 6 Pages 466-470
Published: December 25, 1964
Released on J-STAGE: February 07, 2018
JOURNAL FREE ACCESSThe authors studied on the cup method for the determination of cyano- and hydroxocobalamin. An agar layer containing 0.13% of 2,3,5-triphenyltetrazolium chloride was poured on the flat surface of the agar layer containing nutrient medium and cell suspension of Lactobacillus leichmannii. After the incubation for 18〜20 hours at 37℃, diameters of the red coloured growth zones of lactobacilli around the cups were measured. Thus, the determination of cyano- and hydroxocobalamin in pure solution were successfully performed in the range of 50〜1000mμg/ml with a little errors, but with regard to the natural product further examination would be necessary.View full abstractDownload PDF (568K) -
Yoshikuni TAMAO, Tadakatsu KATO, Shoichi SHIMIZU, Saburo FUKUIArticle type: Article
1964 Volume 30 Issue 6 Pages 471-475
Published: December 25, 1964
Released on J-STAGE: February 07, 2018
JOURNAL FREE ACCESSIn a preceding paper the authors showed a marked increase in the contents of acid-soluble deoxyribosyl compounds as well as DNA in vitamin B_<12>-starved cells of Lactobacillus leichmannii when incubated with exogenously added cobalamins for 5 hours at 37℃. This paper deals with the study to determine a form of vitamin B_<12>-group compound occured in the bacterial cells during the incubation mentioned above, which seems to be an active form participating in the biosynthesis of deoxyribosyl compounds. The vitamin B_<12>-active substance in the cells was extracted with hot 80% ethanol and separated from a contaminating yellow substance by DEAE-cellulose column chromatography. The vitamin B_<12> contained in the passthrough fraction behaved similarly with DBCC in paper electrophoresis performed at pH 2.7 and 3.5. Its absorption spectrum and the changes by illumination as well as by cyanidetreatment were identical with those of DBCC. Furthermore its coenzyme activity was demonstrated to be almost the same as that of DBCC in Abeles・Lee's diol-dehydrase system. From these results it would be concluded that DBCC is the single form of the vitamin B_<12> in the cells of Lactobacillus leichmannii and it participates in the transformation of ribonucleotide (or ribonucleoside) to deoxyribonucleotide (or deoxyribonucleoside).View full abstractDownload PDF (834K) -
Yoshiaki SAITO, Isao SAITO, Teijiro KAMIHARA, Saburo FUKUIArticle type: Article
1964 Volume 30 Issue 6 Pages 476-481
Published: December 25, 1964
Released on J-STAGE: February 07, 2018
JOURNAL FREE ACCESSThis paper describes synthesis and some properties of a mixed disulfide of lipoic acid and pantetheine. Pantetheine was prepared from pantethine by reduction with Na_2S, then reacted with 2 moles of DL-α-lipoic acid dissolved in a NaHCO_3 aqueous solution by being allowed to stand for 3 to 4 hours at pH 7.4 at room temperature. The reaction product showed the behaviors corresponding to the compound, which consisted of one mole of lipoic acid and one mole of pantetheine with KCN-nitroprusside test, thiol exchange reaction by cysteine treatment, bioautography using lipoic acid-requiring microorganisms (Stc. faecalis 10 C1 and Coryneb. bovis) and pantetheine-responding microorganisms (L. bulgaricus B-1 and L. arabinosus) as test organisms. Its ultraviolet and infrared absorption spectra were consistent with the structure proposed above. The growth effect of the mixed disulfide on lipoic acid-requiring microorganism, e. g. Stc. faecalis, was significantly greater than that of α-lipoic acid or a mixture of α-lipoic acid and pantethine.View full abstractDownload PDF (756K)
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