VITAMINS

VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

J-STAGE home
/
VITAMINS
/
All issues

Search

OR

Browse

Search

Search
Browse

All issues

Volume 64, Issue 2

Displaying 1-31 of 31 articles from this issue

Download citation

Show all abstracts

|<
<
1
>
>|

The Role of _L-Ascorbic Acid in the Proline Hydroxylation Reaction

Rina YU, Tadao KURATA, Nobuhiko ARAKAWA

Article type: Article
1990 Volume 64 Issue 2 Pages 67-76
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_67

JOURNAL FREE ACCESS

Show abstractHide abstract

For further clarification of the role of L-ascorbic acid (AsA) in the proline hydroxylation reaction, the specificity of AsA for the decarboxylation of α-keto-glutarate (KGA) was studied using various reductants AsA and its structural analogs. Decarboxylation of KGA was not observed in the absence of AsA. Erythorbic acid (ErA) was found to be as effective as AsA, and D-ascorbic acid was almost as effective as AsA in the reaction, whereas, thiol compounds showed a very slight accelerating effect on the decarboxylation of KGA. L-scorbamic acid (SCA) or erythroscorbamic acid (ErS), at a concentration 10 folds greater than AsA, showed a decarboxylation level of 40-45% that of AsA. Furthermore, in the presence of AsA, the pH-dependence and concentration effect on the decarboxylation of KGA were different from those in the presence of SCA. Moreover, the Lineweaver-Burk plot of the inhibition by SCA of AsA showed that the mode of interac-tion of SCA with AsA may be apparently noncompetitive. From these results, it is suggested that, due to its plane γ-lactone ring system with an endiol group, AsA is a specifically suitable reducing compound for the proline hydroxylation. AsA is considered to be most effective in its approaching and binding to the enzyme active site and reducing the enzyme bound Fe^<3+>. In this study, it was also found that the uncoupled reaction inevitable occurred during proline hydroxylation and this reaction was accompanied by the oxidation of AsA, thus leading to its consumption.

View full abstract

Download PDF (965K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 77-
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_77

JOURNAL FREE ACCESS

Download PDF (105K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 78-
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_78

JOURNAL FREE ACCESS

Download PDF (120K)
[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 79-90
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_79

JOURNAL FREE ACCESS

Download PDF (1234K)
[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 91-94
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_91

JOURNAL FREE ACCESS

Download PDF (1079K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 95-98
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_95

JOURNAL FREE ACCESS

Download PDF (487K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 99-104
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_99

JOURNAL FREE ACCESS

Download PDF (525K)
[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 105-109
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_105

JOURNAL FREE ACCESS

Download PDF (888K)
[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 110-115
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_110

JOURNAL FREE ACCESS

Download PDF (600K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 116-119
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_116

JOURNAL FREE ACCESS

Download PDF (757K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 120-122
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_120

JOURNAL FREE ACCESS

Download PDF (429K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 123-126
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_123

JOURNAL FREE ACCESS

Download PDF (582K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 127-129
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_127

JOURNAL FREE ACCESS

Download PDF (392K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 130-133
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_130

JOURNAL FREE ACCESS

Download PDF (458K)
[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1990 Volume 64 Issue 2 Pages 134-138
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_134

JOURNAL FREE ACCESS

Download PDF (672K)
Photoaffinity Labeling of Thiamin-binding Component in Yeast Plasma Membrane with [^3H]4-Azido-2-nitrobenzoylthiamin

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1990 Volume 64 Issue 2 Pages 139-140
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_139

JOURNAL FREE ACCESS

Download PDF (182K)
[title in Japanese]

Article type: Appendix
1990 Volume 64 Issue 2 Pages 141-145
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_141

JOURNAL FREE ACCESS

Download PDF (447K)
[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 147-148
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_147

JOURNAL FREE ACCESS

Download PDF (303K)
[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 148-149
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_148

JOURNAL FREE ACCESS

Download PDF (326K)
[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 149-150
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_149

JOURNAL FREE ACCESS

Download PDF (324K)
[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 150-
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_150

JOURNAL FREE ACCESS

Download PDF (193K)
[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 151-152
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_151

JOURNAL FREE ACCESS

Download PDF (316K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 152-153
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_152

JOURNAL FREE ACCESS

Download PDF (333K)
[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1990 Volume 64 Issue 2 Pages 153-154
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_153

JOURNAL FREE ACCESS

Download PDF (330K)
[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 154-155
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_154

JOURNAL FREE ACCESS

Download PDF (320K)
[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 155-156
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_155

JOURNAL FREE ACCESS

Download PDF (286K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 157-158
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_157

JOURNAL FREE ACCESS

Download PDF (282K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 158-159
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_158

JOURNAL FREE ACCESS

Download PDF (294K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 159-160
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_159

JOURNAL FREE ACCESS

Download PDF (297K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 160-161
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_160

JOURNAL FREE ACCESS

Download PDF (308K)
[title in Japanese]

[in Japanese]

Article type: Article
1990 Volume 64 Issue 2 Pages 162-163
Published: February 25, 1990
Released on J-STAGE: March 17, 2018

DOIhttps://doi.org/10.20632/vso.64.2_162

JOURNAL FREE ACCESS

Download PDF (266K)

|<
<
1
>
>|

Top