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Volume 42 (1970)
- Issue 6 Pages 349-
- Issue 5 Pages 261-
- Issue 4 Pages 209-
- Issue 3 Pages 141-
- Issue 2 Pages 65-
- Issue 1 Pages 1-
Volume 42, Issue 2
Displaying 1-16 of 16 articles from this issue
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Akio SAKASHITA, Yasuo OSHIMAArticle type: Article
1970 Volume 42 Issue 2 Pages 65-72
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSIn order to elucidate differences in growth-promoting effect of pantothenic acid (PaA) analogues between different types of bifid bacteria, growthstimulating activities of PaA, pantethine (PaSS), pantetheine-4'-phophate (P-PaSH) and CoA on total 66 strains of bifid bacteria (14 strains of type I-II, 20 of type III, 6 of type IV and 26 of type V), which were all isolated from stool of infants were assayed. In addition, peptide requirement of these strains were also determined. PaSS and P-PaSH were active for all the strains of bifid bacteria of each type. CoA was also active for all but 5 strains, whereas PaA was shown to be active for the growth of all the strains of type IV, 60% of type III, 65% of type V, and none of type I-II. Peptide was required for growth of all the strains of type I-II, 15% of type III, 23% of type V and no strain of type IV.View full abstractDownload PDF (723K) -
Takeo ITO, Ritsuko YOSHIMURA, Kenji ARASEArticle type: Article
1970 Volume 42 Issue 2 Pages 73-75
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSPretreatment of milk powder with protease of Streptomyces griseus and Takadiastase B was shown to be necessary to extract thiamine perfectly from milk powder (Vitamins 39,109 (1969)). Further investigation of the procedure demonstrated that extraction of thiamine was most perfect on pretreatment of 5g milk powder with 0.2% Pronase-P at 37℃ for 16 hrs and then with 0.5% Takadiastase for 2-3 hrs. Contamination of thiamine in either Pronase-P or Takadiastase was negligible for estimation. Recovery test of thiamine diphosphate added to the sample was shown to be nearly 100% in the improved procedure, while only 80% recovery in HCl-extraction method.View full abstractDownload PDF (356K) -
Atsuko BABAArticle type: Article
1970 Volume 42 Issue 2 Pages 76-80
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSConcentration of ascorbic acid and glutathione in many flowers having various colors with anthocyan was estimated in order to examine the relation between these compounds and coloration with anthocyan in plants : Flowers in four species, i. e. chrysanthemum, carnation, gerbera and sweet pea were selected for experimental materials and those flowers were classified into several color groups by the 59 standard colors table. The results indicated that anthocyan was much more contained in flowers with deep coloration than in those with light coloration and ascorbic acid was generally much more contained in flowers of deep coloration, rich with anthocyan than those containing no anthocyan. No significant relation between glutathione and coloration was not indicated. Acceleration of physiological function with absorption of infrared ray by anthocyan may be supposed for one of reasons.View full abstractDownload PDF (573K) -
Hiroe KISHI, Satoko NISHIYAMA, Eiichi HIRAOKAArticle type: Article
1970 Volume 42 Issue 2 Pages 81-85
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSDeoxythiamine (DOB_1) having hydrogen in the place of hydroxy group of thiamine (B_1) is a B_1 antagonist. Its inhibition indices on growth and B_1-uptake of Lactobacillus fermenti are about 250 and 6,respectively.^<7)8)> After the everyday subculture of the organism in serial media in increasing concentration of DOB_1,L. fermenti has been mutated. The resistant cells were appeared in the medium containing 1μg of B_1 and more than 800μg of DOB_1 and they were hardly reversible. The resistant cells showed similar B_1 response curve to the original cells and the same level of both B_1-uptake and inhibition of the B_1-uptake by DOB_1 as the original. The most distinguished character of the resistant cells is that they exhibit good growth in the medium containing a high dose of DOB_1 (more than 500μg per 4ml broth) and no B_1. It has been postulated that there is a new metabolic pathway of DOB_1 which may be converted to TDP and no change at the site of B_1-uptake system.View full abstractDownload PDF (539K) -
Masahiro HIRASHIMAArticle type: Article
1970 Volume 42 Issue 2 Pages 86-94
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSAn improved method for the preparation of the 2-oxoglutarate dehydrogenase complex from pig heart muscle that catalyzes CoA-and NAD^+-linked oxidative decarboxylation of 2-oxoglutarate. The highly purified complex has a sedimentation coefficient (s^0_20,W) of 35.7 S and a diffusion coefficient (D_<20>, w) of 1.18×10^<-7>cm^2/sec. The molecular weight was calculated to be 2.8 million from sedimentation and diffusion coefficients and also to be 2.7 million from the data by the Archibald method. A highly purified preparation of the complex contained 6 moles of protein-bound TDP, 6 moles of bound lipoic acid and 9 moles of bound FAD per mole of the complex. This enzyme complex showed absolute dependence on added CoA and NAD^+ in the over-all reaction and showed partially dependence on added TDP in the 2-oxoglutarate dehydrogenase activity. The 2-oxoglutarate dehydrogenase activity of this enzyme complex was activated by Ca^<2+> than Mg^<2+>. Physicochemical data and enzymatic activities obtained in the present investigation are compared with those reported by Sanadi et al. and by Massey.View full abstractDownload PDF (1253K) -
Shuichi KIMURAArticle type: Article
1970 Volume 42 Issue 2 Pages 95-98
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSAlbino rats were fed on a pantothenic acid-deficient diet, supplemented in their diet with the antimetabolite, ω-methyl-pantothenic acid. After three months the rats on pantothenic acid-deficient diet were shown to be in appearance of fatty livers. It was observed that significant lowering of the relative ratios of stearic to palmitic acid and arachidonic to linoleic acid on the fatty acid composition of the liver lipids from pantothenic acid deficient animals. This fact may propose an hypothesis that pantothenic acid-deficiency reduce an activity of the metabolic process involved chain-elongation of fatty acid. A column chromatographic analysis on the liver lipids showed that the relative portion of phospholipid fraction was significantly lower in deficient animals than in the control animals. The determination of the fatty acid composition in these fractions suggested that the patterns of fatty acid in liver lipids were affected remarkably with the relative portions of phospholipids contained in their liver lipids.View full abstractDownload PDF (457K) -
Fumio UEDA, Teruo MAKINO, Atsumi KAZAMAArticle type: Article
1970 Volume 42 Issue 2 Pages 99-106
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSIt was found to permit a distinct separation of vitamin D from vitamin A alcohol by gel filtration utilizing Sephadex LH-20 as bed and chloroform as eluting solvent. But when applied to mixtures of vitamins A alcohol and D in the ratios usually found in pharmaceutical dosage forms, it was not possible entirely to eliminate interfering vitamin A degradation products which moved at the same rate as vitamin D. Effective removal of this interfering substances was achieved with Florex XXS column chromatography described by Schmall^<7)> with a slight modification. Taking these findings into consideration, a method for the determination of vitamin D in the presence of vitamin A involving gel filtration is proposed : After the gel filtration on Sephadex LH-20 column for removal of vitamin A alcohol from vitamin D, a persistent interfering band accompanying vitamin D is eliminated subsequently by means of weakened Florex XXS column containing 7% of water. Vitamin D is determined by applying Wilkie's color inhibitor and method of calculation and Nield's antimony trichloride reagent. Satisfactory recovery was shown in the experiments of mixtures of vitamins A alcohol and D (ratio (IU) of A to D ; 20 : 1).View full abstractDownload PDF (891K) -
Fumio UEDA, Teruo MAKINO, Atsumi KAZAMAArticle type: Article
1970 Volume 42 Issue 2 Pages 107-111
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSThe determination of vitamin D in pharmaceutical preparations by the method described in Vitamins 42,99 (1970) was examined. Hydrolysis of the sample was carried out in ethanolic potassium hydroxide by addition of antioxidant such as mixture of hydroquinone and pyrogallol. The unsaponifiable fraction was fractionated on Sephadex LH-20 column as the gel bed and chloroform as the eluting solvent. The eluted vitamin D was purified by chromatography on the weakened Florex XXS column. The antimony trichloride reagent of Nield with acetic anhydride color inhibitor and the two wave-length reading procedure provided corrected values of vitamin D in the presence of interfering substances. Analytical results of the method applied to a series of pharmaceutical products were almost satisfactory.View full abstractDownload PDF (525K) -
Kiyoshi HARADA, Ryoichi HIGUCHI, Kiyoshi FUKUDA, Isamu UTSUMIArticle type: Article
1970 Volume 42 Issue 2 Pages 112-117
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSSince we have confirmed the finding of Kawasaki et al. that some disulfidetype thiamine derivatives are reduced to thiazole type-thiamines by the incubation with Bacillus thiaminolyticus and decomposed subsequently with thiaminase, the effect of the microorganisms on the gastrointestinal absorption of thiamine-HCl and O-benzoylthiamine disulfide (BTDS) has been investigated. When the microorganisms are given to the albino rats for a week prior to an administration of thiamine-HCl, there is a significant decrease in urinary and fecal recovery of thiamine. On the other hand, a significant decrease in the urinary recovery is not observed when BTDS is given to the animals with the microorganisms, indicating that the absorption of BTDS, one of the lipophillic and easily absorbable thiamine derivatives, is hardly influenced by the presence of the microorganisms in digestive tract. In addition, it is found that the decrease in the absorption of thiamine-HCl seems to be restored by the administration of Lactobacillus spores.View full abstractDownload PDF (708K) -
[in Japanese]Article type: Article
1970 Volume 42 Issue 2 Pages 117-
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSDownload PDF (165K) -
Kenji KUSABAArticle type: Article
1970 Volume 42 Issue 2 Pages 118-132
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSLight and electron microscopical studies have been performed on the cutaneous lesions of the rats fed on vitamin A deficient diet. Histologically, the skin of the vitamin A deficient rats revealed marked atrophy of the epidermis, loss of the granular layer and follicular hyperkeratosis with keratotic plugs. In the epidermis no clear difference between the basal and the prickle cells was demonstrated. In these cells remarkable vacuolar appearance in the cytoplasma and pyknotic change in the nuclei were observed. The dermis showed slight edema and the swelling of the collagen fibers was slight but demonstrable. Marked decrease and atrophy of the hair follicles and the sebaceous glands were observed. The subcutaneous fatty tissue also disclosed marked decrease. Electron microscopical observation exhibited demonstrable changes of the mitochondria in the cytoplasma of the basal and the prickle cells, such as swelling, lower density, vacuolar formation, and irregularity in size and form. Vacuolar formation in the cytoplasma was observed. Desmosomes were intact but the intercellular spaces in the prickle layer were narrow. Tonofibrils attached to desmosomes were short. Decrease in number and size of tonofibrils and keratohyalin granules was also noticed, moreover disappearance of the latter was encountered in many cases. Odland body in the upper prickle cells could not be detected in many cases. Normal keratin pattern of the horny layer was not observed and a curtain lace like structure between the horny and the prickle layers was seen.View full abstractDownload PDF (6337K) -
[in Japanese], [in Japanese]Article type: Article
1970 Volume 42 Issue 2 Pages 133-
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSDownload PDF (181K) -
[in Japanese]Article type: Article
1970 Volume 42 Issue 2 Pages 133-134
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSDownload PDF (324K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1970 Volume 42 Issue 2 Pages 134-
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSDownload PDF (191K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1970 Volume 42 Issue 2 Pages 135-
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSDownload PDF (165K) -
[in Japanese]Article type: Article
1970 Volume 42 Issue 2 Pages 135-
Published: August 25, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSDownload PDF (165K)
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