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Volume 76, Issue 12
Displaying 1-14 of 14 articles from this issue
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[in Japanese]Article type: Article
2002 Volume 76 Issue 12 Pages 581-
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
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Shigeyuki KAWAI, Kousaku MURATAArticle type: Article
2002 Volume 76 Issue 12 Pages 583-594
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSA novel enzyme "inorganic polyphosphate [poly (P) ]/ATP-NAD kinase" that utilizes both poly (P) and ATP as phosphoryl donors was purified from Micrococcus flavus. Another NAD kinase utilizing ATP, but not poly (P), was purified from Escherichia coli, and designated as "ATP-NAD kinase". The results indicated that at least two kinds of NAD kinases "poly (P) /ATP-NAD kinase" and "ATP-NAD kinase" exist in biological systems. The gene for poly (P) /ATP-NAD kinase was cloned from M. flavus (mfnk) and Mycobacterium tuberculosis H37Rv (ppnk), and that for ATP-NAD kinase was from E. coli (yfiB) and Saccharomyces cerevisiae (UTRI). Regardless of the difference of phosphoryl donor specificities, the primary structures of these NAD kinases (Mfnk, Ppnk, YfjB, and Utrlp) were highly conserved. Other than NAD kinase activity, the bacterial Mfnk and Ppnk, and yeast Utrlp showed NADH kinase activities, although YfjB did not. Furthermore, YfjB was inhibited allosterically by NADH and NADPH, suggesting that different regulation mechanisms for NAD (P) H synthesis are operating in microorganisms. Highly efficient mass production system of NADP with Ppnk was established and the system was industrialized.View full abstractDownload PDF (1330K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
2002 Volume 76 Issue 12 Pages 595-600
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (711K) -
[in Japanese]Article type: Article
2002 Volume 76 Issue 12 Pages 601-605
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (526K) -
[in Japanese]Article type: Article
2002 Volume 76 Issue 12 Pages 607-609
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (361K) -
[in Japanese], [in Japanese]Article type: Article
2002 Volume 76 Issue 12 Pages 609-613
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (608K) -
[in Japanese]Article type: Article
2002 Volume 76 Issue 12 Pages 613-615
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (313K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2002 Volume 76 Issue 12 Pages 617-618
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (280K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2002 Volume 76 Issue 12 Pages 617-
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (154K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2002 Volume 76 Issue 12 Pages 618-619
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
2002 Volume 76 Issue 12 Pages 619-620
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (294K) -
[in Japanese]Article type: Article
2002 Volume 76 Issue 12 Pages 620-621
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (231K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
2002 Volume 76 Issue 12 Pages 620-
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (173K) -
[in Japanese]Article type: Article
2002 Volume 76 Issue 12 Pages 621-
Published: December 25, 2002
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (105K)
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