VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 22, Issue 1

Displaying 1-50 of 89 articles from this issue

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STUDIES ON THE METABOLISM OF VITAMIN A, ADMINISTERED IN EXCESS AMOUNT : ESPECIALLY ON THE CHANGES OF VITAMIN A ESTER AND ALCOHOL CONTENTS OF BLOOD SERUM AND VARIOUS TISSUES

Toshio TAKAI

Article type: Article
1961Volume 22Issue 1 Pages 1-10
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_1

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 10-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_10_1

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 10-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_10_2

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STUDIES ON RETICULO-RUMEN DIGESTION : (XIX) ON THE DISTRIBUTION OF THE VITAMIN B_<12>-LIKE SUBSTANCES OF GOAT UNDER NORMAL FEEDING

Makoto KANDATSU, Toshio OZAKI, Bumpei MORI

Article type: Article
1961Volume 22Issue 1 Pages 11-15
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_11

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The rumen contents were taken at regular intervals through a permanent fistula of goat under normal feeding, and fractionated into Infusoria, bacteria, and Infusoria-bacteria free fractions. The vitamin B_<12> activities of each fraction were measured by using Euglena as a test organism and the following results were obtained : The vitamin B_<12> activity of rumen contents was 77-115μg per 100g of dry matter 4-7 hours after feeding. It appears that the goat obtained 12-34μg of true vitamin B_<12> per day from the rumen in which the vitamin B_<12>-like substances, containing the vitamin B_<12>itself, were synthesized. The vitamin B_<12>activities of Infusoria and bacteria fractions became maximum 7-8 hours after feeding, and especially the change of the vitamin B_<12> activity of the bacteria fraction was remarkable. The vitamin B_<12> activity of Infusoria-bacteria free fraction was low, but this fraction had a tendency to increase its activity 7-8 hours after feeding.

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 15-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_15

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ON THE COMBINATION OF PROTEIN AND THIAMINE : (IV) INVESTIGATION ON THE NATURE OF COMBINED PROTEIN BY PAPER ELECTROPHORESIS

Hiroyasu FUKUBA, Yoshiko IWATA, Choten INAGAKI

Article type: Article
1961Volume 22Issue 1 Pages 16-21
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_16

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The nature of the complex formed with protein and thiamine was further investigated by the aid of paper electrophoresis. The fractions obtained by the addition of ammonium sulfate to glycinin solution at 10-60% saturation on the salt behaved almost the same against the electrophoresis. Thiamine adsorbed glycinin gave two patterns on the paper. By the comparison of these with that obtained from glycinin, it was revealed that the one, which was removed a little, was due to the fraction born by the treatment with thiamine and not shown in the paper of glycinin itself.

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ON THE COMBINATION OF PROTEIN AND THIAMINE : (V) MECHANISM OF ADSORPTION OF THIAMINE ON PROTEIN (2)

Hiroyasu FUKUBA, Yohko FUJII, Choten INAGAKI

Article type: Article
1961Volume 22Issue 1 Pages 21-25
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_21

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The complex fomation of protein with thiamine or its derivatives was found to be much influenced with the pH values, and, at neutral ranges, thiamine and dibenzoylthiamine were almost entirely adsorbed on protein and thiamine propyldisulfide was adsorbed about 30%, while, at the extreme acidity (pH 1.0), these three compounds and also pyrimidine and thiazole moieties of thiamine were not adsorbed. At neutral, protein could adsorb not only thiamine but also the components of this, and, at relatively higher concentrations, thiazole moiety was more adsorbed on protein than pyrimidine, while, at lower concentrations, this was in reverse.

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 25-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_25_1

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 25-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_25_2

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STUDIES ON VITAMIN REQUIREMENTS FOR POULTRY : (II) REQUIREMENTS OF THIAMINE, RIBOFLAVIN, NICOTINIC ACID, PANTOTHENIC ACID, CHOLINE, FOLIC ACID, BIOTIN AND VITAMIN B_<12> FOR WHITE LEGHORN CHICKS

Minoru YOSHIDA, Hiroshi MORIMOTO

Article type: Article
1961Volume 22Issue 1 Pages 26-30
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_26

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The requirements of pantothenic acid, folic acid, biotin and vitamin B_<12> for the maximum growth of White Leghorn male chicks in Japan were observed to be not more than the requirements recommended by National Research Council, U.S.A., that is 9.3,0.55,0.088 and 0.0088 mg/kg, respectively, while the requirements of thiamine, nicotinic acid and choline were significantly higher than those recommended in U.S.A., i.e.1.8,26.5 and 1,320 mg/kg, respectively. Riboflavin requirement of our chicks was between 4.5-5.5 mg/kg, which is 1.5-2.0 times more than that recommended in U.S.A. With riboflavin supplementation of 2.9 mg/kg, the amount recommended in U.S.A., typical curled-toe paralysis was observ d, indicating that chicks in this country need more riboflavin than those in U.S.A. This finding with riboflavin is similar to that with pyridoxine reported previously.

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 30-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_30

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THE INFLUENCE OF THIAMINE ON THE METABOLISM OF ZINC : (I) STUDY ON THE DETERMINATION OF ZINC

Kinuko TOMITA

Article type: Article
1961Volume 22Issue 1 Pages 31-34
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_31

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The author employed diantipyrylmethylmethane for the determination of zinc, because it is more simple and sensible than the dithizone method in the organs or urine.

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 34-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_34_1

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 34-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_34_2

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THE INFLUENCE OF THIAMINE ON THE METABOLISM OF ZINC : (II) THE INFLUENCE OF THIAMINE ADMINISTRATION ON THE ZINC METABOLISM IN MAN

Kinuko TOMITA

Article type: Article
1961Volume 22Issue 1 Pages 35-40
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_35

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In order to study the effect of thiamine upon the zinc metabolism, the change of zinc excretion in urine caused by the administration of thiamine or thiamine propyldisulfide was observed in man. When 10 mg of thiamine propyldisulfide was administered intravenously or orally, the urinary excretion of zinc decreased remarkably. But the effect of an intravenous administration of 10 mg thiamine was not remarkable.

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THE INFLUENCE OF THIAMINE ON THE METABOLISM OF ZINC : (III) THE INFLUENCE OF THIAMINE ADMINISTRATION ON THE ZINC AMOUNT OF ANIMAL TISSUES

Kinuko TOMITA

Article type: Article
1961Volume 22Issue 1 Pages 40-49
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_40

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Studies were made, using young rats, on the changes of zinc metabolism by the thiamine deficiency or by the daily administration of 100 mg of thiamine or thiamine propyldisulfide. A significant decrease of the zinc content of organs in thiamine deficiency was observed. At the early stage of thiamine deficiency, the urinary excretion of zinc increased, when the growth was still maintained. When thiamine propyldisulfide was orally administered, the zinc content of organs increased more significantly than by a thiamine administration. These findings indicate a close interrelationship between the activity of thiamine in body and the zinc metabolism.

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 49-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_49_1

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cis-Isotachysterol_2

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 49-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_49_2

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STUDIES ON THIOTHIAMINE : (VI) DETERMINATION OF THIOTHIAMINE IN NATURAL FOODSTUFFS

Takashi SUHARA, Nobuko IRITANI

Article type: Article
1961Volume 22Issue 1 Pages 50-54
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_50

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We worked out three different procedures to determine thiothiamine in acidic aqueous extracts of natural foodstuffs in the presence of thiothiamine. Thiothiamine is oxidized into thiochrome by either (1) alkaline benzoyl peroxide or (2) cyanogen bromide at pH 3.5 to avoide thiochrome-formation from thiamine, then the thiochrome formed is estimated fluorometrically. The third method, microbiological assay of thiamine by Lactobacillus fermenti before and after SO_2-treatments, which converts thiothiamine into thiamine, of the extracts at pH 4.5,will indicate the existence of thiothiamine, if the thiamine values after the treatment is greater than that before. Among 14 kinds of vegetables investigated, thiothiamine was detected in onions, Welsh onions, scallions, cabbage and Brussels spronts by benzoyl peroxide method. Existence of thiothiamine was only confirmed by microbiological assays in onions and scallions, while it failed in Welsh onions, cabbages and Brussel sprouts. Cyanogen bromide method (2) applied to onions and cabbages showed both positive.

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INFLUENCE OF D-SORBITOL AND OTHER CARBOHYDRATES ON THIAMINE ABSORPTION

Eisuke KATSURA, Akiko NAKAMURA, Nobuko ISHIZAKI

Article type: Article
1961Volume 22Issue 1 Pages 54-59
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_54

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It has been reported that D-sorbitol enhances the absorption of vitamin B_<12> from the intestine (Greenberg, 1957,Chow, 1956,1958 et al.). The question of whether this effect of D-sorbitol is concerned only with vitamin B_<12> absorption or also with other kinds of vitamins is interesting and still controversial. For the purpose of clarifying this, clinical studies on the influence of D-sorbitol and various carbohydrates on the thiamine absorption were made. Human subjects were administered orally, in fasting state, in the early morning, 20 mg of thiamine with or without 5 g of D-sorbitol or carbohydrates. Amonunts of thiamine excreted in the urine during 24 hours following the administration were measured. The results obtained indicate that the absorption of thiamine can be enhanced not only by the addition of D-sorbitol, but also by that of D-glucose, D-fructose, inositol and DL-glucosamine, whereas D-mannitol and D-xylose do not have such an effect.

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ON THE LESION OF DIGESTIVE ORGANS IN THE EXPERIMENTALLY PRODUCIED VITAMIN E DEFICIENT RATS, ESPECIALLY OF THE LIVER AND PANCREAS

Ninki HO

Article type: Article
1961Volume 22Issue 1 Pages 59-67
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_59

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Histopathological studies were performed on the digestive organs of experimentally produced vitamin E deficient rats. Atrophy of glandular cells was found in stomach, and desquamation of epithelial cells and inflammatory cell infiltration of submucosa were observed in bowel. Fatty degeneration and necrosis of liver cells were found in the central area of lobules. This may be produced by vitamin E deficiency only, and does not necessitate a lack of sulfur-containing amino acids. In the pancreas, necrosis of pancreatic cells was found, suggesting a possibility to fibrosis. The-vitamin E deficiency is considerd as one of the causative fators for human pancreatic necrosis.

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 67-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_67_1

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 67-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_67_2

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 67-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_67_3

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STUDIES ON THE INFLUENCE OF VITAMIN D ON THE FEMALE SEXUAL FUNCTION OF RATS : (III) EFFECT OF ESTROGEN AND VITAMIN D ON THE FEMALE SEXUAL FUNCTION OF RATS

Toshi ONIWA

Article type: Article
1961Volume 22Issue 1 Pages 68-84
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_68

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The author studied the influence of vitamin D on the sexual function of albino rats. An administration of vitamin D to the castrated animal prevents the atrophy of the uterus to a considerable degree. Administration of an optimal dose of vitamin D accelerates the growth of ovarian follicles and uterus of noncastrated young and adult animals, on the contrary, an excess of vitamin D, especially when administered for a long period, restrains and suspends the sexual function of the animal. Young animals administered with a large dose of vitamin D show the temporary acceleration of sexual function, but the successive administration of an excess-dose causes the disorder of sexual function prior to that of general conditions. In general the effect of administration of vitamin D to the young animals is more prominent than that to the adult ones. The action of estrogen is enhanced by the simultaneous administration of an adequate dose of vitamin D, but it is reduced by a large dose.

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 84-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_84_1

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 84-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_84_2

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STUDIES ON THE MECHANISM OF RIBOFLAVIN BIOSYNTHESIS BY ASHBYA GOSSYPII : (III) EFFECTS OF GROWTH INHIBITORS ON THE RIBOFLAVIN FORMATION

Hiromichi ONOZAKI

Article type: Article
1961Volume 22Issue 1 Pages 85-89
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_85

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As previously reported, the temperature have a remarkable influencnce for the riboflavin biosynthesis in the submerged culture of Ashbya gossypii In this paper, the experiments were performed whether any correlation exists between the riboflavin biosynthesis and cell growth. The influence of several growth inhibitors upon the growth and the riboflavin formation were examined. A remarkable increase of riboflavin production was found in a glucose medium containing 10^<-5>M of NaN_3. From the result obtained, it may be concluded that the stimulating effect of NaN_3 for riboflavin biosynthesis is concerned with the production or decomposition of 〜ph compound by the organism.

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STUDY ON THE PHOTOLYSIS OF FLAVINADENINEDINUCLEOTIDE

Hiroshi KOYAMA

Article type: Article
1961Volume 22Issue 1 Pages 90-95
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_90

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The velocity of photolysis of FAD was compared with those of FMN and riboflavin. The solutions of the substances were saturated with nitrogen or carbon dioxide and exposed to sunlight. The velocity of photolysis was FMN>riboflavin>FAD, and was higher in nitrogen than in carbon dioxide. An unknown substance having a Rf 0.08 (butanol・acetic acid・water) was separated by paper chromatography in the photolytic products. This compound contained phosphoric acid, ribose, adenine and is supposed to be deutero-derivative of FAD.

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 95-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_95_1

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 95-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_95_2

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HISTOCHEMICAL STUDY ON VITAMIN B_6

Wenchu HUNG

Article type: Article
1961Volume 22Issue 1 Pages 96-107
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_96

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A fluorescence microscopic method was applied for the histochemical studies of vitamin B_6. The techniques of the method were described. The distribution of vitamin B_6 in various tissues of albino rat was studied histochemically, and revealed that vitamin B_6 is distributed abundantly in liver, kidneys, and heart, followed by in stomach, small intestine, pancreas, spleen and brain. It was interesting that the distribution of vitamin B_6 was recognized remarkably in β-cells of Langerhans islets. From the histochemical pictures of vitamin B_6 in normal and alloxan-diabetic rats, the absorption, storage and excretion in living bodies were discussed.

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ON THE HISTOLOGICAL DISTRIBUTION OF VITAMIN B_6 IN RATS INTOXICATED WITH CARBON TETRACHLORIDE

Wenchu HUNG

Article type: Article
1961Volume 22Issue 1 Pages 108-110
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_108

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The distribution of vitamin B_6 was observed histochemically in animals intoxicated with carbon tetrachloride. In these animals the intestinal absorption, phosphorylation in liver cells and reabsorption from the epithelia of urinary tubules were impeded, followed by the remarkable decrease of the vitamin B_6 content in each organ.

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 110-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_110_1

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 110-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_110_2

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 110-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_110_3

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 111-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_111_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 111-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_111_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 111-112
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_111_3

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 112-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_112_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 112-113
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_112_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 113-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_113_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 113-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_113_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 113-114
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_113_3

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 114-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_114_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 114-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_114_2

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 114-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_114_3

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 114-115
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_114_4

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[title in Japanese]

[in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 115-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_115_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1961Volume 22Issue 1 Pages 115-
Published: January 23, 1961
Released on J-STAGE: December 18, 2017

DOIhttps://doi.org/10.20632/vso.22.1_115_2

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