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Toyohiko KOMATSU
Article type: Article
1961 Volume 23 Issue 1 Pages
1-4
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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In a previous paper it was shown that FAD in aqueous solution decomposed into riboflavin-4', 5'-cyclic phosphate and adenosine-5'-phosphate when it was kept for a long time. It was found that the decomposition of FAD was greatly accelerated by the presence of trace amounts of heavy metal ions which were brought into the solution from the glass container. The FAD was more stable in the polyethylene container than in the glass. The decomposition of FAD in water was inhibited by adding a small amount of ethylendiaminetetraacetate.
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Tsuyoshi SAIKI
Article type: Article
1961 Volume 23 Issue 1 Pages
4-13
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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A continuous daily administration of 20,000 I.U. of vitamin A or D to rats caused a decrease of body weight and led the animals to death at the advanced stage of the hypervitaminosis. By the heavy dose of vitamin A, levels of haemoglobin and erythrocyte were lowered at the early stage and then a temporary rise was observed, followed by a remarkable decrease at the advanced stage. Vitamin D had no effect on the haemoglobin level, but caused a gradual increase of erythrocyte number. Leucocyte number was also found to be small at the advanced stage in the vitamin A hypervitaminosis. In the case of vitamin D, an increase of leucocyte number was observed at the early stage, followed by a continuous decrease. In both cases, the changes of leucocyte number were found to be mainly those of lymphocyte number. A decrease of nuclear cell number occurred at the early stage of the vitamin A in the bone-marrow hypervitaminosis. After a temporary restoration, a gradual decrease was observed at the advanced stage. The changes were those of levels of erythroblast and granulocyte. On the contrary, in the vitamin D hypervitaminosis an increase of the cell number was observed at the early stage, followed by a gradual decrease. The changes in this case were found to depend mainly on those of erythroblast number.
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
13-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
13-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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Tsuyoshi SAIKI
Article type: Article
1961 Volume 23 Issue 1 Pages
14-24
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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A heavy administration of vitamin K_4 to young or adult rats exerted an inhibitory effect on the growth. Levels of erythrocyte and haemoglobin were markedly lowered and a concentration of blood was observed at the advanced stage of the hypervitaminosis. A decrease of leucocyte number was also observed, but a slight increase was detected at the early stage in infant rats. The changes of leucocyte number were mainly those of neutrophilia leucocyte. Nuclear cell number in the bone-marrow was slightly raised at the early stage and then gradually decreased. Changes of erythroblast were most remarkable. In the rat the vitamin K hypervitaminosis occurred most strongly and the function of blood formation was also markedly lowered. A heavy administration of vitamin B_<12> or folic acid was also carried out. However, the water-soluble vitamins had no effect on blood pictures.
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Tadashi KAMIKUBO, Kenji TAKAHASHI
Article type: Article
1961 Volume 23 Issue 1 Pages
24-27
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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It was recognized that the remarkable growth of Ochromonas malhamensis in the absence of cyanocobalamin in the Ford's medium during long incubation was due to methionine added to the medium. On the other hand the addition of methionine proved to improve the growth response of the organism to the vitamin without affecting the inoculated blank. The findings show that Ochromonas seems to have similar requirements for vitamin B_<12> and methionine to E. coli 113-3 or 215.
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Shigetaka FUJIMOTO
Article type: Article
1961 Volume 23 Issue 1 Pages
27-31
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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FMN could be dephosphorylated enzymatically by white strain of Er. ashbyii. In the case of partially purified enzyme from Er. ashbyii, the optimum pH and temperature were found at 9.0 and 55℃, respectively. The enzyme action was inhibited by ethylenediaminetetraacetate, and activated by Mg^<++>. From these results, the enzyme action was considered to be attributed to alkaline phosphomonoesterase. The larger amount of the enzyme was found in white strain than in yellow strain.
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Kazuo HOTTA, Isao ISHIGURO, Zyunko NAITO
Article type: Article
1961 Volume 23 Issue 1 Pages
31-36
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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Experimental studies were made on the change of hepatic enzyme activity in tryptophan metabolism of riboflavin deficient rats, and the pyridoxal phosphate content was determied. The enzymic activity in the liver of riboflavin deficinet rats was decreased remarkably in tryptophan pyrrolase, kynurenine oxygenase and kynurenine transaminase, and slightly kynureninase. On the other hand, the pyridoxal phosphate content in the liver was decreased by 30 percent in the riboflavin deficient rats compared with that of normal rats.
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
36-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
36-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
36-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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Teijiro MIYAMOTO
Article type: Article
1961 Volume 23 Issue 1 Pages
37-42
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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Vitamin B_<12> assay in blood was investigated. It was observed that the following method is most appreciable for many subjects and a long term experiment. Microbiological assay with L. leichmannii in using Nissan media for a stock culture, an inoculum culture and an assay medium are suitable for the purpose of the present experiment. KCN boiling method by Hayashi et. al. is suitable for the extraction of vitamin B_<12> from the whole blood. The alkali stable factor in the whole blood of our samples was negligible. Samples of whole blood can be kept at least for 10 days without changing its vitamin B_<12> content under a frozen state. The preparations of vitamin B_<12> extracted from the whole blood can be stored under toluene at 4℃ for a week, Variations of daily vitamin B_<12> value in whole blood of one subject were less than 20%.
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
42-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
42-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
42-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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Teijiro MIYAMOTO
Article type: Article
1961 Volume 23 Issue 1 Pages
43-48
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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The influence of vitamin B_<12> administration on the values of vitamin B_<12> in blood of boys and girls, 12-13 years of age, was investigated. One tablet of vitamin B_<12> per day was given to 38 children in an orphanage of Osaka for a year and plasebos were given to other 50 children living at the same asylum. The difference between the above two groups of vitamin B_<12> values in whole blood before each administration was not statistically significant. After the administration of 25 μg of vitamin B_<12> per day for two months and a half, the mean value of vitamin B_<12> in whole blood of the children was increased, although some of them did not show any change. The mean value of vitamin B_<12> in whole blood after the administration of vitamin B_<12> for 2.5 months was higher than that of control group, and thereafter the values of the two groups were in parallel. After 3 months discontinuation of the administration, the mean values of vitamin B_<12> concentration in whole blood did not differ significantly between the two groups.
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
48-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
48-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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Kiku MURATA, Satoe HASE
Article type: Article
1961 Volume 23 Issue 1 Pages
49-51
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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The detection of pyrimidinyl-cysteine, as a reaction product from thiamine under the existence of cysteine by partially purified bacterial thiaminase I, was made by paper partition chromatography. The reaction product was positive upon both Dragendorff and ninhydrine reagents, and showed Rf 0.32-0.34 (by n-butanol・methanol・water=2 : 1 : 1,at 15℃ for 16-20 hours). The absorption spectrum of the extract at Rf 0.32-0.34 of the paper chromatogram by 0.001 N hydrochloric acid solution showed maximum at 249 mμ or at 236 mμ and 275 mμ when measured respectively at pH 3.5 or pH 7.0. The types of the spectrum were similar to those of 4-amino-pyrimidine at both pH. From these observations, it was presumed that pyrimidinyl-cysteine can be produced from thiamine and cysteine by the catalytic reaction of bacterial thiaminase I.
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Kiku MURATA, Satoe HASE, Hideo IKEHATA, Shioko MITANI
Article type: Article
1961 Volume 23 Issue 1 Pages
52-55
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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Pyrimidinyl-cysteine, which was produced from thiamine and cysteine by the catalytic exchange reaction of a purified bacterial thiaminase I. was isolated as a crystal, mp. 219-220℃ (decomp.), by the column chromatography using Amberlite IRC-50. The yield was 61%. The crystal showed positive reaction with Dragendorff or ninhydrine reagents, but negative with nitroprusside reaction. Nitroprusside reaction did not appear even after the treatment of the crystal by zinc in acid solution. Therefore, the chemical construction of pyrimidinyl-cysteine was postulated as S-(2-methyl-4-aminopyrimidinyl-5-methyl)-cysteine. The results of elemental analysis of C, H, N and S in the compounds obtained enzymatically and chemically were almost identical with the theoretical value of the formula.
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
55-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
55-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
55-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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Kiyomasa KOMETANI, Masahiko KOSAKA, Taro NAKAMURA, Sho ASAI
Article type: Article
1961 Volume 23 Issue 1 Pages
56-61
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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The isolation of vitamin B_<12> from the activated sludge was examined. The extraction was performed in a pilot plant with hot water under stirring, as previously reported by Kamikubo, Tanaka and Matsuda. Various kinds of filtration process were tried in oder to make filtrate as clear as possible. The filtrate thus obtained was further purified either by active charcoal or ion exchange resin. The filtration process was further facilitated by addition of some precipitants. However, the clear filtrate was not obtained when the moisture content of the dried sludge exceeded 12%. Thus the mean value of vitamin B_<12> content from May 1958 to Sep. 1959 was 4g per ton of the sludge, though a part of vitamin B_<12> in the sludge was lost by rain water when the solar evaporation process was performed in summer.
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
61-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
61-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
61-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
61-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
62-63
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
63-64
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
64-65
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
65-68
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
68-70
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
70-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
70-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
70-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
71-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
71-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
71-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
72-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
72-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
72-73
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
73-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
73-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
73-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
73-74
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
74-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
74-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
74-75
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
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[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 1 Pages
75-
Published: April 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
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