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Volume 47, Issue 7
Displaying 1-14 of 14 articles from this issue
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Toshio ANMO, Mitsunori WASHITAKE, Yasuzi TAKASHIMA, Mitsuko FURUYA, Ke ...Article type: Article
1973 Volume 47 Issue 7 Pages 341-350
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSIn the previous papers, the influences of water contents on the stability of vitamin A acetate and its related compounds in aqueous ethanolic solutions were reported. Similar investigations were carried out on vitamin A aldehyde and the results are described in this paper. Vitamin A aldehyde solutions in absolute ethanol or aqueous ethanol with various water contents were stored at various temperatures. When the remaining rates were estimated by a spectrophotometric method, vitamin A aldehyde in 60〜90% aqueous ethanolic solutions was found to be more stable than vitamin A alcohol solution in the corresponding solutions. The observed transformation patterns of vitamin A aldehyde were the isomerization of all-trans into 13-cis and the others including decomposition. By applying the method using nuclear magnetic resonance (NMR), all-trans vitamin A aldehyde in solutions was confirmed to be isomerized into 13-cds isomer until it reached the equilibrium of all-trans and 13-cis (1 : 0.4). When the stored vitamin A aldehyde solutions were subjected to a thin-layer chromatography, those in 60〜90% aqueous ethanol gave five spots including the two due to all-trans and 13-cis isomers while that in absolute ethanol gave the spots and an additional one (R_F 0.74). The chemical structure of the R_F 0.74 substance was investigated after purifying by a silica gel column chromatography and then it was assigned to have an acetal structure (1) from the results of NMR, UV and mass spectra. Other minor products have not been investigated.View full abstractDownload PDF (821K) -
Masahiko KIKUCHIArticle type: Article
1973 Volume 47 Issue 7 Pages 351-356
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSIn order to clarify the correlation between the free B_1 and its ester, changes of content in embryonal axis, scutellum, and endosperm of maize seed were investigated. In an endosperm, the amount of total B_1 continues to increase in the course of germination ; especially, the rate of increase being remarkable at an early stage, at which the esterification of B_1 also occurs to a remarkable extent. Such an increase of total B_1 may be due to an influx of B_1 from the scutellum. It is surmised that quantitative and qualitative changes of B_1 in endosperm are favorable for the digestion of reserve materials in this organ. In embryonal axis, the content of total B_1 increases in parallel with the growth of axis. The reversion of relative amounts of free B_1 and its ester occurs at an later stage than in the case of the endosperm. In scutellum, the esterification of B_1 gradually proceeds, and the relative amounts of free B_1 and its ester is upset at the stage of 2〜3 cm radicle length. The total B_1 continues to decrease remarkably during germination. This is probably due to the outflow of B_1 onto the endosperm and embryonal axis. From these facts, it is inferred that the scutellum behaves as a reservoir of B_1. TTP begins to occur at the stage of imbibition, whereas TDP at a later stage. It may be that TTP takes an essential part in some metabolic system at an earlier stage of germination.View full abstractDownload PDF (590K) -
Hisateru MITSUDA, Tomonori NADAMOTO, Kenji NAKAJIMA, Kenjiro TADERAArticle type: Article
1973 Volume 47 Issue 7 Pages 357-364
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSAdenine inhibited riboflavin production of Eremothecium ashbyii increasingly with increasing concentration in non-growing cell medium. Riboflavin production decreased 54% by the addition of the purine at 3×10^<-3>M after incubation for 18h. When adenine at 10^<-3>M was added into non-growing cell medium, S-adenosylhomocysteine accumulated at 1.7×10^<-7>mol in cells after incubation for 12h. On the other hand, in the same experiment, a small amount of S-adenosylmethionine accumulated in cells at the start of the incubation. When methionine at 10^<-2>M was added to the medium, riboflavin production was stimulated and at the same time, a large amount of S-adeno-sylmethionine (1.4×10^<-5>mol) accumulated in the cells after incubation for 12h. Further research indicates that S-adenosylhomocysteine inhibits the riboflavin synthesis and S-adenosylmethionine stimulates the synthesis of this vitamin.View full abstractDownload PDF (720K) -
Hisateru MITSUDA, Yoshiyuki NISHIKAWA, Kenji NAKAJIMAArticle type: Article
1973 Volume 47 Issue 7 Pages 365-372
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSXanthine brought about a high riboflavin production, while caffeine showed a drastic inhibition of riboflavin production and growth of the microorganism. However, it was elucidated that caffeine inhibited directly riboflavin production and, as a result, the growth of this organism was inhibited. In non-growing cells, the same results as those observed in growing cells were obtained. It seems possible that the inhibition of caffeine is decreased competitively by xanthine. Methylxanthines (theophylline, theobromine and caffeine) were absorbed into cells, at a significantly slower rate than xanthine during non-growing cell incubation and their ultraviolet absorption spectra were not changed with the incubation. Furthermore, the addition of xanthine and caffeine brought about the noticeable enhancement of the GTP pool and a marked shift towards ATP in the intracellular adenosine nucleotide pool at 18h after incubation.View full abstractDownload PDF (782K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1973 Volume 47 Issue 7 Pages 373-
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (180K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1973 Volume 47 Issue 7 Pages 373-374
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 7 Pages 374-
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (199K) -
[in Japanese]Article type: Article
1973 Volume 47 Issue 7 Pages 374-375
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (331K) -
[in Japanese]Article type: Article
1973 Volume 47 Issue 7 Pages 375-
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (182K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 7 Pages 375-376
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (271K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 7 Pages 376-
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (134K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 7 Pages 377-
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 7 Pages 377-378
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (270K) -
[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 7 Pages 378-
Published: July 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (151K)
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