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Volume 70, Issue 10
Displaying 1-23 of 23 articles from this issue
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Shozo YAMAMOTO, Kei YAMAMOTOArticle type: Article
1996 Volume 70 Issue 10 Pages 453-463
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSFatty acid cyclooxygenase transforms arachidonic acid to prostaglandin (PG) H_2, which is a precursor for various bioactive prostaglandins and thromboxanes. Recent studies have demonstrated the occurrence of two isozymes of the cyclooxygenase enzymes (COX-1 and COX-2). COX-1 is a constitutive enzyme, which is found in many mammalian tissues and engaged in various house-keeping functions there. In contrast, COX-2 is a product of an immediate early gene, and induced rapidly and transiently in certain types of cell. An osteoblastic cell line MC3T3-E1 produced PGE_2 from a arachidonic acid, and its cyclooxygenase enzyme was induced by EGF, TGFβ, TNFα, epinephrine and prostaglandins with a lag phase of 1-2 h. Several lines of evidence indicated the identity of the induced enzyme as COX-2 isoform. The mechanism of transcriptional regulation of the COX-2 gene was investigated with TNFα as an inducer. The 5'-flanking region of the COX-2 gene was cloned, and shown by deletion analyses to include two positive regions with NFκB and NF-IL6 elements. Point mutations of these elements and gel shift assays suggested a potential role of both NFκB and NF-IL6 in the TNFα-dependent induction of COX-2 in MC3T3-E1 cells.View full abstractDownload PDF (1083K) -
Keiko TAZUYA, Kazuko YAMADA, Hiroshi KUMAOKAArticle type: Article
1996 Volume 70 Issue 10 Pages 465-471
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSThe biosynthetic pathway of the pyrimidine moiety of thiamin in Saccharomyces cerevisiae, a eucaryote, is different from that of procaryotes. In procaryotes, the pyrimidine moiety is synthesized from 5-aminoimidazole ribonucleotide, an intermediate in purine biosynthesis. This has been demonstrated by investigating the nutritional requirement of adenine- and thiamin-requiring mutants of Salmonella typhimurium and by tracer experiments with radioactive compounds in Escherichia coli and S. typhimurium. We investigated pyrimidine biosynthesis in S. cerevisiae by GC-MS analysis using a stable isotope labeled tracer. Origin of the C-4 of pyrimidine was determined by a radioactive tracer method. These experiments showed that in S. cerevisiae, the pyrimidine moiety is synthesized from histidine and pyridoxine.View full abstractDownload PDF (556K) -
[in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 473-
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (162K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1996 Volume 70 Issue 10 Pages 473-474
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (281K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 474-475
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (298K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1996 Volume 70 Issue 10 Pages 475-476
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (298K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 476-
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (172K) -
[in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 476-477
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (200K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 479-
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (155K) -
[in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 479-480
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (277K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 480-481
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (287K) -
[in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 481-482
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (300K) -
[in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 482-483
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (305K) -
[in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 483-
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (173K) -
[in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 484-
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (180K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 484-486
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (438K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 486-
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (182K) -
[in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 486-487
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (257K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 489-490
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (291K) -
[in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 490-491
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (239K) -
[in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 493-494
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (294K) -
[in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 494-497
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (510K) -
[in Japanese]Article type: Article
1996 Volume 70 Issue 10 Pages 497-500
Published: October 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (388K)
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