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Masato OKAMURA
Article type: Article
1981 Volume 55 Issue 11 Pages
495-508
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
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Various conditions for reducing dehydroascorbic acid (DAsA) were studied, using dithiothreitol (DTT). This led to an improved method for the separate determination of ascorbic acid (AsA) and DAsA in blood plasma, and the specific method for determination of total AsA in urine. (1) Separate determination of AsA and DAsA in blood plasma. The content of AsA was determined by α,α'-dipyridyl method (A). DAsA was reduced to AsA by incubation with DTT. After removing the excess DTT with N-ethylmaleimide (NEM), total AsA, i.e. reduced plus original, were determined by theα,α'-dipyridyl method (B). The amount of DAsA could then be calculated from these results (B minus A). (2) Determination of total AsA in urine. The urine sample was acidified with trichloroacetic acid and was shaken with activated carbon to remove interfering substances. As AsA was oxidized with this treatment, the resultant DAsA was reduced by the DTT-NEM system for determination byα,α'-dipyridyl method.
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Susumu HAMAJIMA, Shigeru ONO
Article type: Article
1981 Volume 55 Issue 11 Pages
509-515
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
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Some drugs which provoke hepatomegaly or hepatic injury, were tested for their effects on the FMN and FAD-synthesizing activities in the liver of male rats. Flavokinase and FAD pyrophosphorylase activities were found to be decreased by carbon tetrachloride or ethionine; whereas phenobarbital which induces drug metabolizing enzymes, increased the activities of both flavokinase and FAD pyrophosphorylase in the liver with increasing concentrations of the drugs. On the other hand, 3-methylcholanthrene, one of the carcinogenic polycyclic hydrocarbons, which was known to induce only some specific drug metabolizing activities, showed no significant enhancement in activities of these two enzymes. Phenobarbital elicited no longer distinct induction of the activities of flavokinase and FAD pyrophosphorylase in the vitamin B_2-deficient rat. These results indicate that induction of drug metabolizing flavin enzyme activities by phenobarbital might cause not only the increment of the flavin enzyme apo-proteins, but also increase the activities of FMN and FAD synthesizing enzymes.
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Tsukasa KATSUKI, Soutomi INN, Ryohei OGURA
Article type: Article
1981 Volume 55 Issue 11 Pages
517-522
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
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Effects of vitamin B_2-butyrate and ubiquinone-10 (UQ_<10>) on adriamycin (ADM)-induced cardiac mitochondrial disorders were studied in male Wistar rats given daily injection of ADM (4 mg/kg body weight) for one week. Administration of ADM resulted in uncoupling of oxidative phosphorylation and in an increase of lipid peroxide of cardiac mitochondria. Measurement of UQ homologues in cardiac mitochondria by high-pressure liquid chromatography showed that UQ_<10> content decreased markedly after ADM administration. On the other hand, simultaneous administration of vitamin B_2-butyrate and UQ_<10> with ADM improved the oxidative phosphorylation activities and prevented the lipid peroxide formation of mitochondria, and furthermore restored the UQ_<10> content up to the normal level. Thus, combination therapy of both the vitamins was expected to prevent the ADM-induced cardiotoxicity during cancer chemotherapy.
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Kazuko YAMADA, Kazuya UCHIDA, Hiroshi KUMAOKA
Article type: Article
1981 Volume 55 Issue 11 Pages
523-528
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
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It has been proposed that the pyrimidine moiety of thiamin and purine share the early part of their biosynthetic pathways in bacteria. Newell and Tucker had reported that the last common intermediate is 5-aminoimidazole ribonucleotide (AIR) on the basis of its satisfaction on the requirements both for a purine and for pyrimidine moiety of thiamin of some double-requirement mutants of Salmonella typhimurium. We investigated the possibility of 5-aminoimidazole-4-carboxylic acid ribonucleotide (AICAR) as common inter-mediate by using radioactive bicarbonate. Radioactive carbon of bicarbonate was not incorporated into pyrimidine and thiazole moieties of thiamin in Escherichia coli and Saccharomyces cerevisiae, while the carbon was incorporated into AMP from RNA. These results show that their biosynthetic routes do not contain CO_2-fixation reaction and AICAR is not a last common inter-mediate in E. coli.
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Tadashi KOBAYASHI, Toshio OKANO
Article type: Article
1981 Volume 55 Issue 11 Pages
529-536
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
537-
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
538-541
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
541-545
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
545-549
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
550-555
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
555-559
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
560-562
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
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Article type: Appendix
1981 Volume 55 Issue 11 Pages
563-567
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
569-570
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
570-571
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
571-572
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
572-
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], Jack R. Cooper
Article type: Article
1981 Volume 55 Issue 11 Pages
572-573
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
573-574
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
574-
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
575-576
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
576-578
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
578-579
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1981 Volume 55 Issue 11 Pages
579-580
Published: November 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL
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