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Volume 65, Issue 1
Displaying 1-9 of 9 articles from this issue
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Haruo MISONOArticle type: Article
1991 Volume 65 Issue 1 Pages 1-12
Published: January 25, 1991
Released on J-STAGE: March 17, 2018
JOURNAL FREE ACCESSLysine dehydrogenase, which has been purified to homogeneity from A. tumefaciens, consisted of two identical subunits. The enzyme showed a high substrate specificity for L-lysine. The preincubation of the enzyme with L-lysine resulted in a 2-fold increase in the activity. The activation resulted from the association of two dimeric enzymes to form a tetramer. Upon modification of SH groups of the enzyme with 5, 5'-dithiobis (2-nitrobenzoate), neither the interconversion between the dimer and the tetramer nor the activation by L-lysine occurred. In addition to L-lysine, several compounds, which have both a carboxyl group and a hydrophobic side chain, activated the enzyme. The binding experiments showed that the presence of one catalytic site and two activator binding sites in the dimeric enzyme. In the enzyme reaction, the pro-R hydrogen at the prochiral C-6 carbon of L-lysine was specifically abstracted and transferred to the pro-R position at C-4 of the nicotinamide moiety of NAD^+. The enzyme could be used for the spectrophotometric assay of L-lysine.View full abstractDownload PDF (1143K) -
Kazumasa HIRAUCHI, Toshiyuki SAKANO, Shigeru NOTSUMOTO, Tadayoshi NAGA ...Article type: Article
1991 Volume 65 Issue 1 Pages 13-21
Published: January 25, 1991
Released on J-STAGE: March 17, 2018
JOURNAL FREE ACCESSDownload PDF (770K) -
Keizo UMEGAKI, Msato KUROKAWA, Hiromi SAEGUSA, Tomio ICHIKAWAArticle type: Article
1991 Volume 65 Issue 1 Pages 23-29
Published: January 25, 1991
Released on J-STAGE: March 17, 2018
JOURNAL FREE ACCESSCarbon tetrachloride (CCl_4) was administered to rats at a single dose of 2500 mg/kg or a daily dose of 200 mg/kg for 9 days, and the changes of serum lipid peroxide levels and their relation to hepatic lipid peroxide levels were investigated. The effect of vitamin E (E : α-Tocopheryl acetate, 80 mg/kg) administration on the lipid peroxide levels was also examined. CCl_4 administration increased the levels of TBARS and triglyceride in liver and GOT and GPT activities in serum, while TBARS levels in serum were decreased. In case of the 9-day dosage of CCl_4, the decrease of serum lipids was also observed. These results suggested that the increase of liver TBARS levels did not reflect the serum TBARS levels. Simultaneous administration of E with CCl_4 inhibited the increase in TBARS levels in the liver. E administration also significantly inhibited the increase of serum GOT and GPT following the 9-day dosage of CCl_4.View full abstractDownload PDF (648K) -
Article type: Appendix
1991 Volume 65 Issue 1 Pages 31-34
Published: January 25, 1991
Released on J-STAGE: March 17, 2018
JOURNAL FREE ACCESSDownload PDF (351K) -
[in Japanese]Article type: Article
1991 Volume 65 Issue 1 Pages 35-36
Published: January 25, 1991
Released on J-STAGE: March 17, 2018
JOURNAL FREE ACCESSDownload PDF (221K) -
[in Japanese]Article type: Article
1991 Volume 65 Issue 1 Pages 37-
Published: January 25, 1991
Released on J-STAGE: March 17, 2018
JOURNAL FREE ACCESSDownload PDF (191K) -
[in Japanese], [in Japanese]Article type: Article
1991 Volume 65 Issue 1 Pages 37-40
Published: January 25, 1991
Released on J-STAGE: March 17, 2018
JOURNAL FREE ACCESSDownload PDF (544K) -
[in Japanese]Article type: Article
1991 Volume 65 Issue 1 Pages 40-41
Published: January 25, 1991
Released on J-STAGE: March 17, 2018
JOURNAL FREE ACCESSDownload PDF (285K) -
[in Japanese]Article type: Article
1991 Volume 65 Issue 1 Pages 41-43
Published: January 25, 1991
Released on J-STAGE: March 17, 2018
JOURNAL FREE ACCESSDownload PDF (320K)
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