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Volume 31 (1965)
- Issue 6 Pages 433-
- Issue 5 Pages 343-
- Issue 4 Pages 235-
- Issue 3 Pages 175-
- Issue 2 Pages 107-
- Issue 1 Pages 1-
Volume 31, Issue 6
Displaying 1-8 of 8 articles from this issue
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Tadakatsu KATO, Shoichi SHIMIZU, Saburo FUKUIArticle type: Article
1965 Volume 31 Issue 6 Pages 433-444
Published: June 25, 1965
Released on J-STAGE: February 09, 2018
JOURNAL FREE ACCESSDownload PDF (1396K) -
Seiji ISHIKAWA, Goichiro KATSUIArticle type: Article
1965 Volume 31 Issue 6 Pages 445-448
Published: June 25, 1965
Released on J-STAGE: February 09, 2018
JOURNAL FREE ACCESSMethod of separation and determination of mixed tocopherols by gas chromatography using a hydrogen flame ionization detector was investigated. Analysis were carried out on the column packing consisted of Chromosorb W coated with 1.5% SE-30 at a column temperature of 250℃. For the determination of each tocopherol, squalene was used as an internal standard, and the half value width method as a method of measurement of peak areas. After mixed tocopherols were acetylated by the solution of pyridine-acetic anhydride, they were dissolved in acetone, and 5μl of 3% acetone solution of mixed tocopherols was injected into gas chromatography. By this method satisfactory results were obtained with the linearity of calibration curves from 20 to 100 μg of each tocopherol and with the recovery of 96.0 - 103.0%.View full abstractDownload PDF (444K) -
Osamu TANAKAArticle type: Article
1965 Volume 31 Issue 6 Pages 449-463
Published: June 25, 1965
Released on J-STAGE: February 09, 2018
JOURNAL FREE ACCESSThe clinical and experimental effects of lipoic acid, dihydrolipoic acid and thiamine-8-(methyl-6-acetyldihydrothioctate) disulfide (TATD) have been investigated. Dihydrolipoic acid lowers the oxgen consumption and basal metabolic rate in the hyperthyroidic patient. Lipoic acid decreases the oxygen consumption and basal metabolic rate in the diabetic patient. A large dose of lipoic acid or a moderate dose of dihydrolipoic acid lowers the acetylating potency. TATD is hydrolysed in the presence of cell membrane into its components, thiamine and dihydrolipoic acid, and the former is mainly found in the cell, while the latter remains in the medium. On the oral administration of TATD, dihydrolipoic acid appears in the blood of portal vein. Dihydrolipoic acid inhibits the oxydative glycolysis of pyruvic acid in the liver. Intravenous injection of dihydrolipoic acid or TATD aggravates the galactose tolerance. Thus, a great care has to be taken by the clinical use of these drugs, concerning the dosage and the method of administration.View full abstractDownload PDF (1734K) -
Keiichi KOHNOArticle type: Article
1965 Volume 31 Issue 6 Pages 464-469
Published: June 25, 1965
Released on J-STAGE: February 09, 2018
JOURNAL FREE ACCESSWhen thiamine benzyldisulfide or thiamine propyldisulfide was incubated with denatured ovalbumin, bovine serum alubumin, or β-lactoglobulin at neutral pH for one hour, formation of free thiamine as well as protein-bound alkylmercaptan was demonstrated. Formation of free mercaptan was also observed in some cases, but no protein-bound thiamine was produced in contrast with the previously reported case of symmetric thiamine disulfide such as O-benzoyl-thiamine disulfide or thiamine disulfide. The ratio of the bound mercaptan to free thiamine slightly varied with the species of protein and was shown to be dependent on the initial molar ratio of the vitamin derivative to protein. From these results it was proved that thiamine alkyldisulfide of asymmetric type reacted with protein-SH mainly as follows, producing protein-alkylmercaptan mixed disulfide. Protein-SH + thiamine-S-S-R &irarr; Protein-S-S-R + thiamine-SH The difference between the reaction patterns of these asymmetric and symmetric thiamine disulfide was briefly discussed.View full abstractDownload PDF (732K) -
Keiichi KOHNOArticle type: Article
1965 Volume 31 Issue 6 Pages 470-480
Published: June 25, 1965
Released on J-STAGE: February 09, 2018
JOURNAL FREE ACCESSSymmetric thiamine disulfides such as TDS, BTDS, PrTDS or BuTDS and asymmetric thiamine disulfides such as TBzD or TPD were found to be useful as the reagents for determination of SH groups. Stoichiometry, specificity, and sensitivity were tested with cysteine and glutathione and a standerd procedure was established. Usually 0.1〜0.2 μmoles of SH was incubated with 5 μmoles of TDS in the presence of EDTA at pH 6〜9 (optimal 7〜8), 30℃ for 5〜30 minutes, and the amounts of SH was calculated from the amounts of free thiamine liberated. In microprocedure, 0.002 μmoles thiol could also be determined with considerable accuracy. Sulfhydryl contents of ovalbumin, bovine serum albumin and β-lactoglobulin, determined by this method after sufficiently denatured, were confirmed to agree with those obtained by the Boyers' PCMB method. Reactivity between protein-SH and these thiamine disulfides as well as some possible use of the method were discussed.View full abstractDownload PDF (1279K) -
Isamu UTSUMI, Kiyoshi HARADA, Keiichi KOHNOArticle type: Article
1965 Volume 31 Issue 6 Pages 481-486
Published: June 25, 1965
Released on J-STAGE: February 09, 2018
JOURNAL FREE ACCESSBinding of thiol-type thiamine with S-S groups of natural protein through thiol-disulfide exchange reaction, was investigated. When native or denatured bovine serum albumin (BSA) were incubated with its 10-160 molar amounts of thiamine at pH 9,37℃ for 1〜24 hours, about 0.6〜3.5 moles of bound thiamine (disulfide type) per mole of BSA was detected. Reduction of BSA greatly reduced the complex formation, suggesting involvement of the protein S-S groups. In an anaerobic conditions, however, the bound thiamine was much decreased and it was suggested that the thiamine disulfide, produced by air oxidation, was concerned with the large amounts of bound thiamine above mentioned. Sephadex gel filtration of the anaerobically incubated reaction mixture, followed by determination of SH groups showed that about 0.3 S-S groups of BSA was reduced. From these results it was supposed that thiamine itself could react only in some definite extent with S-S groups of BSA.View full abstractDownload PDF (755K) -
Isamu UTSUMI, Kiyoshi HARADA, Keiichi KOHNOArticle type: Article
1965 Volume 31 Issue 6 Pages 487-493
Published: June 25, 1965
Released on J-STAGE: February 09, 2018
JOURNAL FREE ACCESSIt was found that the protein-bound thiamine of maximum 4.5 moles per mole of protein was produced by the reaction of thiamine disulfide or O-benzoylthiamine disulfide with SH- blocked bovine serum albumin at pH 9,37℃ for 4〜24 hours. The complex formation was inhibited by addition of SH reagent such as p-chloromercuribenzoate or N-ethylmaleimide, whereas greatly accerlated by an addition of minute amount of thiol-type thiamine. These findings suggest the similar mechanism of the complex formation to those of low molecular disulfide-disulfide systems, where trace amounts of thiols catalyze the disulfide exchange reaction. Iodine-oxidized egg albumin also produced small amounts of the bound thiamine. Asymmetric disulfide-type thiamines (thiamine alkyldisulfide) were found to form both protein-bound alkylmercaptan and the dound thiamine under the same conditions. The third types of thiamine-protein complex forming reactions were considered as the following equations.[chemical formula][chemical formula]View full abstractDownload PDF (885K) -
Takesi HUKUHARA, Hiroyuki FUKUDAArticle type: Article
1965 Volume 31 Issue 6 Pages 494-499
Published: June 25, 1965
Released on J-STAGE: February 09, 2018
JOURNAL FREE ACCESSUtilizing Trendelenburg's as well as Magnus' method the influence of TTFD upon the movement of the isolated small intestine of guinea pigs, rabbits, cats and dogs were studied. The results were summarized as follows : (1) At the concentration of 5 × 10^<-5> g/ml TTFD produced, on the one hand, a lowering of the tone, prolongation of the period of rhythmic contraction waves and on the other hand, a remarkable increase of the amplitude of the waves. (2) After the administration of atropine, the excitatory effect of TTFD was reversed to the inhibitory. (3) After successive administrations of atropine and hexamethonium, no action of TTFD was remarked. (4) TTFD had no effect on the small intestine isolated from cats treated with reserpine and atropine. (5) From the results described above, it may be concluded that TTFD exerts an exciting action upon both the excitatory and inhibitory neurones residing in Auerbach's plexus, whereas it exerts no action upon the intestinal muscle. It could be considered that the effects described in (1) were the results of a mutual coordination of the function of two kinds of neurones described above.View full abstractDownload PDF (720K)
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