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Masao SHIMIZU
Article type: Article
1971 Volume 44 Issue 1 Pages
1-13
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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Based on the findings during the course of studies on biosynthetic pathway and chemical synthesis of CoA, the author has been interested in elucidating the structural requirement of CoA for the coenzyme activity. Although the central role of the thiol group in an acyl-transfer system was already established, the function of the pantetheine and the nucleotide moieties of CoA has remained uncertain. The author focussed on the kinetic analysis of the effects of various CoA analogs on a CoA-dependent enzyme phosphotransacetylase. It was concluded that the nucleotide moiety of CoA is important for recognition of this coenzyme by phosphotransacetylase, while the pantetheine moiety may chiefly contribute to the binding strength of CoA with the enzyme.
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Tetsuro KITAZAKI
Article type: Article
1971 Volume 44 Issue 1 Pages
14-25
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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The absorption of B_<12> was studied in Mongrel dogs using ^<57>Co-cyanocobalamin. It was found that the main absorptive site is the terminal ileum, and that absorption of small doses (0.005〜0.03μg) from isolated ileum, is usually poor with considerable fluctuation, while that of doses above 0.1μg is greater percentage-wise. Dog gastric juice contains one or two B_<12> binding proteins which are relatively resistant to proteases with respect to binding capacity, and when placed in intestinal loops with the test dose, it inhibits absorption. Under the same conditions, rat stomach mucosa extract rather promoted absorption of the small dose. Dog duodenal fluid collected after pancreatic secretion stimulation, enhanced absorption of small doses of B_<12>, whether it waa used in the intestinal loop or in the small intestine without the supply of pancreatic juice. It seems that the gastric binder has an unknown role and that pancreatic juice has a prime function in B_<12> absorption in the dog.
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Ikuo NISHIGAKI, SyedH. HASAN, Akira KOTAKI, Kunio YAGI, Sumihiko OKUYA ...
Article type: Article
1971 Volume 44 Issue 1 Pages
26-30
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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Electron microscopy of dietary fatty liver showed deposition of many fat droplets in the cytoplasm and disorderly arrangement of intracellular organelles including the lamellar structure of the rough-surfaced endoplasmic reticulum. By treating rats for a week with myoinositol, reduction of fat droplets and return of the lamellar structure of the rough-surfaced endoplasmic reticulum to normal were evident. Histological comparison of the liver sections before and after the perfusion of isolated dietary fatty liver indicated that myoinositol added to the perfusate accelerated the removal of fats from the liver to the perfusate. This was confirmed by chemical analysis of the perfusate before and after the perfusion. With liver slices, a similar acceleration, by addition of myoinositol, in removal of fats from cells to medium, was observed.
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Fumio UEDA, Teruo MAKINO, Atsumi KAZAMA, Kayoko WATANABE
Article type: Article
1971 Volume 44 Issue 1 Pages
31-36
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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In Order to eliminate α-tocopherol from vitamins A and D, application of ion exchange chromatography using strongly basic anion exchange resin Amberlyst A-26 with iso-propanol was examined. α-Tocopherol was adsorbed on the Amberlyst A-26 column, whereas unadsorbed vitamins A alcohol and D were eluted quantitatively by subsequent washing of the column with iso-propanol. Although a large part of α-tocopherol was removed by ion exchange chromatography, some α-tocopherol-degradation products, Which cause interference in the color reaction by SbCl_3 acetyl chloride reagent, were eluted with viamins A alcohol and D. However both the degradation products of α-tocopherol and A alcohol were separated from vitamin D by gel filtration on the Sephadex LH-20 column in chloroform. When α-tocopherol was removed from vitamin D by ion exchange chromatography on Amberlyst A-26 prior to the purification by the gel filtration and the Florex XXS adsorption chromatography (Vitamins 42,99 (1970)) was used, the recovery of vitamin D from mixtures contaning D_2 and α-tocopherol was almost satisfactory. This method will be applicable to the determination of vitamin D in the presence of vitamin A and α-tocopherol.
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Hideo SHINOZAKI, Masao MORITA, Jiro SUGIMOTO, Mitsuhiro NAGATA
Article type: Article
1971 Volume 44 Issue 1 Pages
37-43
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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Changes of potassium and sodium contents in isolated guinea-pig atria kept in modified Locke's solution without KCl were prevented by thiamine tetrahydrofurfuryl disulfide (TTFD) added to the medium. This effect was seen, also, at absence of TTFD in the medium when atria were pretreated with TTFD. These effects were not seen when thiamine or DMPD (dimethalium propyl disulfide) was used in place of TTFD. Transportation of TTFD, thiamine or DMPD into atria was tested by means of those labeled compounds with ^<14>C. ^<14>C-Thiamine was identified in atria treated with ^<14>C-TTFD. Transportation of TTFD into atria was far markedly more than that of thiamine or DMPD.
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Akito YOSHIMURA
Article type: Article
1971 Volume 44 Issue 1 Pages
44-51
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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The behavior of ^<57>Co-B_<12> in rat intestinal loops in the absence of intrinsic factor (IF) has been investigated and the following results were obtained. 1) Small amounts of B_<12> placed in the loop are rendered non-dialyzable and unabsorbable within a short period of time. 2) There seems to be a rather constant amount of B_<12> binding capacity in loop washing at any time, although it is small, and it is produced and released into the lumen from the mucosa. There may be an equilibrium between production and degradation of the binder. 3) The loop is capable of inactivating a vast amount of B_<12>, if time is allowed, suggesting stability of binder conjugated with B_<12>. 4) In vitro systems the binding capacity of mucosal homogenates increases when incubated with Krebs-Henseleit bicarbonate glucose compared with physiological saline, and the same is true with mucosa cell suspensions. A chemical reaction involving glucose may be required in such increase of binding capacity.
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
52-
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
52-53
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
53-
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
53-54
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
54-
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
55-
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
55-
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
56-57
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
57-
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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[in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
57-58
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
58-59
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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[in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
59-
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
59-60
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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[in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
60-
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
60-61
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
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[in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
61-
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1971 Volume 44 Issue 1 Pages
61-62
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1971 Volume 44 Issue 1 Pages
62-63
Published: July 25, 1971
Released on J-STAGE: February 27, 2018
JOURNAL
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