All issues
Volume 41, Issue 1
Displaying 1-10 of 10 articles from this issue
- |<
- <
- 1
- >
- >|
-
Ichiro KUROKAWA, Toshiyuki KIMURA, Tatsuo NAGAI, Mizuo KAMIMURAArticle type: Article
1970 Volume 41 Issue 1 Pages 1-4
Published: January 31, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSThe influence of vitamin E on the ADP induced platelet aggregation was studied. Platelet rich plasma (PRP) was prepared from the blood obtained from normal person with 3.8% of trisodium-citrate as anticoagulant. α-Tocopherylacetate was added with the concentration of 0.1,0.2 and 0.5 mg/ml PRP, 2 hours prior to the addition of ADP (1 μg/ml PRP), and the same amount of solvents contained in 0.1,0.2 and 0.5 mg of tocopherol, respectively to 1 ml of PRP. 0.15,0.3 mg of α-tocopherylacetophosphate were added to PRP 1 ml, respectively, just prior to the addition of ADP. The grade and duration of platelet aggreagtion was measured with Shimadzu-Bauschlomb Spectrophotometer at 610 mμ. 0.5 mg of α-tocopherylacetate, 0.15 mg and 0.3 mg of α-tocopherylacetophosphate seemed evidently to inhibit the ADP induced platelet aggregation, and rather promototed its effect in every other cases.View full abstractDownload PDF (486K) -
Seiichi NAMBARUArticle type: Article
1970 Volume 41 Issue 1 Pages 5-9
Published: January 31, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSThe growth of E. coli B was inhibited by some analogues of nicotinic acid (NiA) or nicotinamide (NAA) in the following order : pyridazine-3-carboxamide (Pyd-3-CAA)> 6-aminonicotinamide (ANNA)> pyridazine-3-carboxylic acid (Pyd-3-CA). Pyd-3-CAA was found to be the most potent inhibitor for E. coli B.. The inhibition was recovered by NAA slightly better than NiA, and by quinolinic acid (QA) in higher concentrations. However, Pyd-C-3-AA did not exhibit any inhibitory effect to L. arabinosus 17-5 and Leuc. mesenteroides ATCC 9135. Another inhibitor of amido type, ANAA, also didnot inhibit the growth of the both bacteria. On the contrast, Pyd-3-CA inhibited competitively the growth of the Leuc. mesenteroides ATCC 9135 (inhibition index, about 760) as well as the L. arabinosus 17-5,the latter was reported previously.View full abstractDownload PDF (446K) -
Akira HATANO, Toshio KATOArticle type: Article
1970 Volume 41 Issue 1 Pages 10-14
Published: January 31, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSAutooxidation of ascorbic acid is initiated by dehydrogenation of the hydroxyl groups coupled to the 2- and 3-C atoms. The difference between the highest occupied energy and the lowest occupied energy becomes close when 60 atom and 70 atom coupled to the 1- and 2-C atoms form anion radicals and ring opening of the endiol group becomes difficult. The electron affinity of 4-C atom increases remarkably when double bond forms between 60 atom and 70 atom coupled to the 1- and 2-C atoms and the ring of the endiol group breaks. After this, decomposition progresses toward the direction of stable nuclear configuration.View full abstractDownload PDF (518K) -
Kikuko INOUEArticle type: Article
1970 Volume 41 Issue 1 Pages 15-20
Published: January 31, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSMany reports reveal the fact that acylthiamine derivatives, such as BTDS or BTMP produce O-benzoylthiamine (OBT) directly or indirectly as a intermediate compound in the course of the formation of thiamine in the living body. But owing to the fact that OBT changed to thiochrome by the thiochrome reaction using BrCN and NaOH, the differential determination between thiamine and OBT was impossible. As the results of the investigation for the reaction condition with OBT, the author have found a suitable condition which forms benzoylthiochrome and established a differential determination between OBT and thiamine. The condition found by the author is as follows : (1) Benzoylthiochrome is formed from OBT when NH_4OH is used instead of NaOH after the addition of BrCN. (2) Benzoylthiochrome is easily extracted in ethyl acetate, but on the contrary to this thiochrome is hardly extracted in ethyl acetate.View full abstractDownload PDF (582K) -
Tatsuro MIYAHARAArticle type: Article
1970 Volume 41 Issue 1 Pages 21-25
Published: January 31, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSAs thiamine and antithiamine compounds were accumulated increasingly on the cells during the time-course of incubation with Kloeckera apiculata in the culture medium, competitive inhibition of cell-uptake of thamine by pyrithiamine or imidazolothiamine compounds was observed at the initial stage of accumulation (Kurata, et al. : Vitamins 36,388,1967). At the later stage of accumulation on larger amounts of the yeast, antithiamine compounds were more completely uptaken by the cells, resulting in less inhibition of thiamine-uptake by cells. At the initial stage of accumulation (5 min.), the cell-uptake of thiamine by Kl. apiculata on incubation with thiamine and antithiamine compounds (both 10^<-6> mole) (A) and the cell-uptake of antithiamine compounds on incubation with Kl. apiculata (B) were estirnated ; the values A/B were compared with their 50% growth inhibition indices ; pyrithiamine 0.51 : 38,benzylimidazolothiamine 1.43 : 380,butylimidazolothiamine 1.60 : 700,imidazolothiamine 1.61 : 3500,triazinothiamine 2.10 : 52000. Fairly close correlation between the values A/B and the inhibition indices was demonstrated as shown in Fig. 2.View full abstractDownload PDF (527K) -
Chikataro KAWASAKI, Tomio NAGAYAMAArticle type: Article
1970 Volume 41 Issue 1 Pages 26-29
Published: January 31, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSAs O-benzoylthiamine (OBT) was shown to be more inhibitory to thiamine-uptake by the cells of Lactobacillus fermenti than pyrithiamine, toxicity and biological activity of OBT were checked on animals. Acute toxicity of OBT on mice was proved to be nearly equal to or a little weaker than thiamine-HCl. Growth of rats on thiamine-deficient diet was stimulated by oral daily dose of 10 μg Eq. OBT but not by 5 μg Eq.-dose, while the growth on 5 μg -dose of thaimine-HCl was shown to be equal to that on 10 μg Eq.-dose of OBT. Weight-gain of the thiamine-deficient rats on single dose of 100 μg Eq. OBT was smaller and shorter in their duration than that on thiamine-HCl. When 5 mg Eq./kg of OBT or thianime-HCl were orally given to rats, the increase of blood thiamine contents after one hour was significatly lower than that by thiamine-HCl but when given to chicken, no significant difference of thiamine-levels in blood was observed.View full abstractDownload PDF (479K) -
Kaname KURIAKI, Yoshihisa NOZAKI, Fumihiko KATO, Shinji USUDA, Masuo M ...Article type: Article
1970 Volume 41 Issue 1 Pages 30-37
Published: January 31, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSThe effects of CCT on the gut of guinea pig, rabbit and rat have been studied. CCT stimulated spontaneous motility of the isolated segment of small intestine, but it was less active with large intestine. Species difference was also observed. The strip of longitudinal muscle prepared from the rabbit ileum was more sensitive to CCT than that of the whole strip, and it was responsive even after storing in the ice box for 48 hours. CCT sensitized the longitudinal muscle to acetylcholine. The muscle strip from the reserpinized rabbit still responded to CCT. Conversely the circular muscle strip did not react to CCT. Stimulant actions of CCT on the isolated ileum of guinea pig were not affected by preincubation with atropine, hexamethonium, diphenhydramine or harmine. CCT, administered i.v., also stimulated the movement of ileum in the rabbit. These findings suggested a myogenic nature of the CCT activity.View full abstractDownload PDF (757K) -
Yoshikuni TAMAO, Yasushi MORIKAWA, Shoichi SHIMIZU, Saburo FUKUIArticle type: Article
1970 Volume 41 Issue 1 Pages 38-44
Published: January 31, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSIt has been known that the three propionamide groups at the positions b, d and e of the corrin ring of cobalamin are easily hydrolyzed and give the mixture of cobalamin carboxylic acids, B_<12>-(COOH)_<1〜3>, possessing 1 to 3 propionic aicd group(s) in place of propionamide group(s). The present paper deals with the in vivo and in vitro formation of the coenzyme form, i.e. 5'-deoxyadenosyl derivative, from each of cobalamin carboxylic acids by Propionibacterium shermanii. When cyanocobalamin mono-, di- or tricarboxylic acid was added to the growing culture of Pr. shermanii, all of these compounds were found to convert to the coenzyme forms almost quantitatively. During the cultivation period, 50 to 70% of the carboxylic groups was amidated. In the case of the incubation of aquocobalamin or its carboxylic acid analogs with the crude extract of the same bacteria, the comparative rates of conversions to the coenzyme forms were as follows : OH-B_<12> 77,OH-B_<12>-(COOH)_1 54,OH-B_<12>-(COOH)_2 32,OH-B_<12>-(COOH)_3 7. With respect to the deoxyadenosylation of the three isomers of aquocobalamin monocarboxylic acid, the conversion rate of b-isomer to the coenzyme form was substantially the same as that of aquocobalamin but d- and e-isomers could hardly serve as the substrates.View full abstractDownload PDF (830K) -
Yoshikuni TAMAO, Yasushi MORIKAWA, Shoichi SHIMIZU, Saburo FUKUIArticle type: Article
1970 Volume 41 Issue 1 Pages 45-50
Published: January 31, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSAnalogs of 5'-deoxyadenosylcobalamin (DBCC) having one to three carboxyl groups at position(s) b, d and/or e on the corrin ring (DBCC-(COOH)_<1〜3>) have been prepared by reacting the corresponding cob(I)alamin carboxylic acids with 5'-iodoadenoisine. In propanediol dehydratase system (EC. 4. 2. 1. 28) the analogs with two or three propionic acid side-chains (DBCC-(COOH)_2 or DBCC-(COOH)_3) did not serve as the coenzyme, whereas all of the three isomers of DBCC-(COOH)_1 were effective. Although K_m values for the three isomers were almost identical (9-16 X 10^<-6> M, i.e. 10 to 20 times that for DBCC), significant differences were observed among the V_<max> values of the reactions dependent on these isomers. The V_<max> values were as follows : DBCC-b-(COOH)_1 (analog having propionic acid side-chain at position b), 96%; DBCC-d-(COOH)_1,57%; DBCC-e-(COOH)_1,17%, as compared with that of DBCC. Three isomers of OH-B_<12>-(COOH)_1 acted as the competitive inhibitors for the enzyme used, and their K_i values were almost similar. The cobalt-carbon bonds of the complexes of the three isomers with apoprotein were irreversibly cleaved under aerobic conditions in the absence of the sustrate as is the case of the normal holoenzyme, and the order of their inactivation rates was in accord with the order of the V_<max> values mentioned above. These results would suggest that the three propionamide side-chains have similar influences on the affinity between B_<12> coenzyme and apoprotein of propanediol dehydratase, and, nevertheless, the modification of the position e causes the most significant decrease in the catalytic activity of the resulting holoenzyme species.View full abstractDownload PDF (653K) -
Yoshikuni TAMAO, Yasushi MORIKAWA, Shoichi SHIMIZU, Saburo FUKUIArticle type: Article
1970 Volume 41 Issue 1 Pages 51-56
Published: January 31, 1970
Released on J-STAGE: February 22, 2018
JOURNAL FREE ACCESSTwo analogs of 5'-deoxyadenosylcobalamin (DBCC), 10-Cl-DBCC and 10-Br-DBCC, in which the hydrogen atom at 10C position on the corrin ring is replaced by the halogen atom, were prepared by the chemical method using 5'-iodoadenosine or enzymatic method using crude enzyme from Propionibacterium shermanii from their corresponding aquo-forms. In propanediol dehydratase system (EC. 4. 2. 1. 28), the K_m values of the analogs were almost identical with that of DBCC but the V_<max> values of the reactions catalyzed by the analog-apoprotein complex were significantly smaller than that of the normal reaction : DBCC 100,10-Cl-DBCC 40,10-Br-DBCC 20. The decomposition rates of the cobalt-carbon bonds by oxygen in the absence of the substrate were in good agreement with the order of the V_<max> values mentioned above. These facts would indicate that the incorporation of the halogen atoms into the corrin ring influences the nature of the cobalt-carbon bond in such a way that the dissociation of the bond for the exhibition of the coenzyme action is rendered unfavorable.View full abstractDownload PDF (650K)
- |<
- <
- 1
- >
- >|