All issues
Volume 28 (1963)
- Issue 6 Pages 465-
- Issue 5 Pages 347-
- Issue 4 Pages 267-
- Issue 3 Pages 211-
- Issue 2 Pages 129-
- Issue 1 Pages 1-
Volume 28, Issue 6
Displaying 1-26 of 26 articles from this issue
- |<
- <
- 1
- >
- >|
-
Hisateru MITSUDAArticle type: Article
1963 Volume 28 Issue 6 Pages 465-479
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSDownload PDF (1519K) -
Minoru TOKUMARUArticle type: Article
1963 Volume 28 Issue 6 Pages 480-487
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSAlthough various thiamine derivatives have been available, effect of the substances on the nervous tissues is not yet clear. In this study, their effects upon the oxygen uptake were investigated according to Peters' catatorulin test, using the slices and homogenates of avitaminous pigeon brain. In an experiment with the slices, it was demonstrated that TPD, TTFD, TATD and TDS were more effective than thiamine hydrochloride in the oxygen uptake. It was also observed that CAT was less effective than the latter, showing little effect of TDP and BTMP. In another experiment with the homogenates, on the contrary, it was recognized that TDP markedly stimulated the oxidation of pyruvate, and the other derivatives above mentioned slightly stimulated similarly to thiamine hydrochloride. A slight inhibition of the effect was noted in the both slices and homogenates added the highly concentrated TPD and TATD, whereas it was not in the concentrated TDS. It appears that TDS is the most effective compound studied for the avitaminous brain.View full abstractDownload PDF (1008K) -
Katsu TAKENOUCHI, Kazuo ASOArticle type: Article
1963 Volume 28 Issue 6 Pages 488-500
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSMeasurement of radioactivity in the organs, urine and feces in men and experimental aminals after oral or intramuscular injection of ^<35>S-labelled thiamine hydrochloride or thiamine propyldisulfide, revealed that the amount of urinary radioactive substances other than thiamine was one third of the total radioactivity. Main fraction among nine radioactive substances in urine was isolated by adsorbing on Amberlite IR-410 resin. This substance, having Rf value 0.7 with water- saturated n-butanol, did not have fluorescence under ultraviolet light, and was negative for thiochrome test, indicated not having thiamine molecule by bioautography, and did not behave like any known derivatives or decomposition products of thiamine.View full abstractDownload PDF (1541K) -
Katsu TAKENOUCHI, Riichi IMAI, Kazuo ASO, Shoji OKAMOTO, Takemasa KOBA ...Article type: Article
1963 Volume 28 Issue 6 Pages 501-507
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSAs preliminary studies on the clinical application for the treatment of pigmented dermatosis with a massive doses of ascorbic acid (1〜2g), participations of ascorbic acid in the formation of melanin as well as absorption and excretion of the vitamin in man and experimental animal were investigated. Ascorbic acid inhibited melanin formation in tyrosine-tyrosinase system by its reducing property, and the reaction did not proceed untill all ascorbic acid in the reaction system was oxidized to dehydroascorbic acid. The minimum amount of complete inhibition of melanin formation was 0.5μM. The amounts of ascorbic acid in the skin after intramuscular injection of 300 mg to guinea pig or rabbit were 3.0μM. There was no marked difference in amount between pigmented and nonpigmented skin. The level of ascorbic acid did not precede than 2.4〜2.6mg%, whenever massive amount was given orally. Urinary excretion of ascorbic acid was 20% of the amount administered after daily oral administration of 2g for one month.View full abstractDownload PDF (830K) -
Katsu TAKENOUCHI, Kazuo ASO, Yasuaki SHIRATORI, Takaya KOBAYASHI, Shin ...Article type: Article
1963 Volume 28 Issue 6 Pages 508-516
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSThe present authors examined the radioactive substances in urine after intravenous injection of lipoic acid-^<35>S to healthy men. About 10 radioactive substances were detected on actigram and radioautogram. The radioactivity of injected lipoic acid-^<35>S was almost recovered in 24 hours urine. Sulfate-^<35>S was small in urine. Amoung 10 radioactive substances, Rf 0.1,0.6 and 0.75 (developed with water-saturated n-butanol) were main products (about 32% of the total activity). Rf 0.6 and 0.75 substances responded for Streptococcus faecalis R or Coryne-bacterium bovis, which are thought to be conjugated forms of lipoic acid, and free lipoic acid was small as 0.3% of the administered amount.View full abstractDownload PDF (1499K) -
Kenzo KATOArticle type: Article
1963 Volume 28 Issue 6 Pages 517-521
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSIn the hypophysectomized rat, GOT activities of liver gradually decreased and reached to approximately half the normal value 30 days after the operation. On the contrary, no influence of hypophysectomy was recognized on brain GOT activities.View full abstractDownload PDF (596K) -
Kenzo KATOArticle type: Article
1963 Volume 28 Issue 6 Pages 522-524
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSNo change of GOT activities was observed in liver and brain of sham-hypophysectomized rat after 30 days. In the case of normal rat, GOT activities of liver slightly increased through the same reduced diet intake as with the hypophysectomized rat. However, no influence of the reduced diet intake described above was observed on the enzyme activities in brain.View full abstractDownload PDF (352K) -
Kenzo KATOArticle type: Article
1963 Volume 28 Issue 6 Pages 525-530
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSIt was noticed that the addition of pyridoxal phosphate to liver homogenate of hypophysectomized rat was effective to recover the decreased GOT activities almost completely among 15 days after the operation. After the limited period (approximately 15 days) this effect of pyridoxal phosphate was seldom recognized. Oral administration of pyridoxine to hypophysectomized rat was effective for the recovery of the enzyme activities of liver, too, but the effect was weaker than that of the pyridoxal phosphate described above.View full abstractDownload PDF (621K) -
Kenzo KATOArticle type: Article
1963 Volume 28 Issue 6 Pages 531-534
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSHepatic GOT activities of normal rat increased slightly as a result of successive intramuscular injections of ACTH (1 unit per day). Effect of ACTH injections on GOT activities of liver in hypophysectomized rat was more remarkable than that of addition of pyridoxal phosphate in vitro or pyridoxine administration in vivo, described previously. It was concluded from the authors' results that hepatic GOT activities was controlled by hypophysic-adrenal system and discussed on this regulation mechanism from the viewpoint of relation to vitamin B_6 metabolism in these reports. In addition, it was noticed that the attitude of GOT of brain in hypophysectomized rat was quite different from that of liver. This result is likely due to the difference of relation between pyridoxal phosphate and apo-GOT molecule, tissue to tissue.View full abstractDownload PDF (466K) -
Soichi NIWA, Nobu KATAYAMAArticle type: Article
1963 Volume 28 Issue 6 Pages 535-540
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSThe influence of mixing of raw materials and wrapping materials for the loss of vitamin C and the fading of food color was studied with several powdered juices, enriched by vitamin C. Among several wrapping materials examined, wrapping by double layer of aluminium foil and polyethylene laminated cellophane were found to be most suitable to prevent the decomposition of vitamin C and the fading of colouring materials during the preservation. As for the mixing constituents, citric acid had considerable influence for the stabilities of vitamin C and food colors.View full abstractDownload PDF (675K) -
Chikataro KAWASAKI, Takamitsu SHIMAMOTO, Eiichi HIRAOKAArticle type: Article
1963 Volume 28 Issue 6 Pages 541-545
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSThiamine propyldisulfide (TPD) was more effective than thiamine on the growth of Lactobacillus fermenti 36 in cysteine (+)- or Na_2S_2O_3 (+)-medium, and thiamine disulfide (TDS) was as effective as thiamine. Same results were observed in the preincubated cysteine (+)- or Na_2S_2O_3 (+)- medium with TPD or TDS in which TPD or TDS was completely changed into thiamine. TPD or TDS preincubated with only cysteine was as effective as thiamine on the growth of L.fermenti. It was confirmed by using TPD-^<35>S (outer) that S-propylmercapto-L-cysteine was produced from TPD in cysteine (+)-medium, but this product had no effect on the growth of L.fermenti.View full abstractDownload PDF (479K) -
Hiroe KISHI, Eiichi HIRAOKAArticle type: Article
1963 Volume 28 Issue 6 Pages 546-548
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSPhysical and chemical properties of deoxythiamine (DOB_1) were almost similar to those of thiamine. Prebulda and formaldehyde azo methods of thiamine determination were tried to DOB_1 and it was found that the color substances which were produced from DOB_1 were very similar to those from thiamine in UV spectrum and in paper partition chromatography. DOB_1 was completely oxidized with BrCN to deoxythiochrome and when reacted with benzenesulfonyl chloride, it was changed to a thiochrome-negative compound as thiamine was. In nonenzymatic system of thiamine which produces acetoin through acetolactate from pyruvate, DOB_1 produced about 70% of acetoin which thiamine did.View full abstractDownload PDF (362K) -
Hiroe KISHI, Eiichi HIRAOKAArticle type: Article
1963 Volume 28 Issue 6 Pages 549-553
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSTen mg of deoxythiamine (DOB_1) was given to rats subcutaneously and its metabolites in urine were determined. About 70% of given DOB_1 was excreted in the first five hour urine as DOB_1. LD_<50> of DOB_1 for mice was as follows : 0.34 mg/g for the normal, 0.16mg/g for the thiamine deficient for 6 days, 0.22 mg/g for the thiamine deficient for 14 days. The toxicity of DOB_1 to mice was not reduced by preinjection of thiamine or thiamine pyrophosphate. The daily intraperitoneal injection of 0.8 mg and 1.0 mg of DOB_1 to mice and rats respectively on thiamine deficient diet failed to promote their growth. However, addition of 5 μg of thiamine to them showed the same growth curve as that responsible to 5μg of thiamine alone. Thus no anti-thiamine activity of DOB_1 was observed.View full abstractDownload PDF (566K) -
Yoshinori ITOKAWAArticle type: Article
1963 Volume 28 Issue 6 Pages 554-557
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSIn order to investigate the physiological properties of thiamine propyldisulfide. TPD-^<35>S was used. This paper deals with studies on the method of determination of TPD-^<35>S and several fundamental experiments. Blood or tissues containing TPD-^<35>S was digested with the mixture of nitric acid and perchloric acid, then ^<35>S was determined as benzidine sulfate-^<35>S. In the case of standard TPD or thiamine solution, ^<35>S was determined directly by thinness method and it was confirmed that the both samples were pure by paper chromatography.View full abstractDownload PDF (426K) -
Yoshinori ITOKAWAArticle type: Article
1963 Volume 28 Issue 6 Pages 558-563
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSThe metabolism of TPD-^<35>S was studied on rabbits. Urinary ^<35>S excretion was measured after oral or parenteral administration of TPD-^<35>S (outer). It was found that a large proportion (about 70%) of propylmercapto radical was excreted in the first 24 hours mainly as sulfate.View full abstractDownload PDF (712K) -
Yoshinori ITOKAWAArticle type: Article
1963 Volume 28 Issue 6 Pages 564-567
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSThe metabolism of thiamine in vivo was investigated after administration of TPD-^<35>S (inner) and thiamine-^<35>S to rabbits. In the case of intravenous injection of TPD-^<35>S urinary ^<35>S excretion was lower than that of thiamine-^<35>S. However, in the case of oral administration, ^<35>S derived from TPD-^<35>S was excreted more than that from thiamine-^<35>S. It was confirmed that in both cases only free thiamine was detected in urine by actigraph.View full abstractDownload PDF (481K) -
Yoshinori ITOKAWAArticle type: Article
1963 Volume 28 Issue 6 Pages 568-573
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSDistribution of ^<35>S in plasma and red cells was investigated after TPD-^<35>S (outer or inner) or thiamine-^<35>S was administrated to rabbits. After oral or parenteral administration of TPD-^<35>S (outer), ^<35>S was found mainly in plasma, and thiamine was mostly in red cell. ^<35>S in plasma was found to be combined with serum albumin. ^<35>S increased remarkably in the red cell after an administration of TPD-^<35>S (inner).View full abstractDownload PDF (659K) -
Yoshinori ITOKAWAArticle type: Article
1963 Volume 28 Issue 6 Pages 574-577
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSFollowing oral administration of TPD-^<35>S (outer), TPD or thiamine to rats, the amounts of total thiamine and ^<35>S in the intestinal wall and its contents were determined. It was found that TPD was mainly absorbed in the upper part of small intestine with in 30 minutes after an oral administration. It was also observed that propysulfide part was absorbed more quickly in intestine than thiamine.View full abstractDownload PDF (452K) -
Yoshikuni TAMAO, Tadakatsu KATO, Shoichi SHIMIZU, Saburo FUKUIArticle type: Article
1963 Volume 28 Issue 6 Pages 578-582
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSPropionibacterium shermanii was cultured for 9 days at 30℃ in the medium to which cyanocobalamin or hydroxocobalamin was added from the beginning of the cultivation or on the 2nd, 4th, 6th or 8th day in the incubation period, respectively. Total amount of vitamin B_<12>-group compounds was assayed spectrophotometrically after conversion to dicyanocobalamin, and their forms were studied by paper ionophoresis and absorption spectrophotometry after fractionation with CM-cellulose. In the case of hydroxocobalamin the greater part of the amount exogenously added on the 8th day was incorporated into the cell and transformed to DBCC as well as from the beginning of the incubation. In the case of cyanocobalamin the vitamin B_<12>-active substances in the cell consisted of cyanocobalamin and DBCC. The amounts were lowered as the time of the addition was delayed. Hydroxocobalamin was not detected in the cell cultivated with cyanocobalamin. From the results it would seem appropriate to conclude that the affinity to the bacterial cell and the rate of conversion to DBCC of cyanocobalamin were smaller than those of hydroxocobalamin.View full abstractDownload PDF (588K) -
Goichiro KATSUIArticle type: Article
1963 Volume 28 Issue 6 Pages 583-591
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSDownload PDF (967K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963 Volume 28 Issue 6 Pages 592-
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSDownload PDF (173K) -
[in Japanese]Article type: Article
1963 Volume 28 Issue 6 Pages 592-
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSDownload PDF (173K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963 Volume 28 Issue 6 Pages 592-593
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSDownload PDF (324K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963 Volume 28 Issue 6 Pages 593-
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSDownload PDF (203K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963 Volume 28 Issue 6 Pages 593-594
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSDownload PDF (323K) -
[in Japanese]Article type: Article
1963 Volume 28 Issue 6 Pages 594-
Published: December 25, 1963
Released on J-STAGE: February 01, 2018
JOURNAL FREE ACCESSDownload PDF (172K)
- |<
- <
- 1
- >
- >|