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Yukiya SAKAMOTO
Article type: Article
1961 Volume 24 Issue 4 Pages
223-237
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Quantitative microassay of pyridoxal phosphate was considered by using apotryptophanase. It is possible to determine to 0.01-0.5μg by this method. Biosynthesis route of pyridoxal phosphate was examined by this method, and it was shown that the route by which pyridoxal phosphate was synthesized from pyridoxine via pyridoxine phosphate was important physiologically. Pyridoxine phosphate is one of flavin enzyme and in the yeast TPN-dependent pyridoxine dehydrogenase and isopyridoxal reductase are consist. In addition the relation between vitamin B_6 and pregnancy, high fat meal, amino acid administration, cancer and adrenal hormone administration was made clear.
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
237-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Akihiro MIURA
Article type: Article
1961 Volume 24 Issue 4 Pages
238-246
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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The absorption, excretion and distribution of radioactive vitamin B_<12> in the body of albino rats were studied. In 24 hours after the oral administration of vitamin B_<12> labelled with Co^<60>, most of it was excreted in feces and only about 12 percent of it was absorbed in the body. The urinary excretion was, however, remarkedly increased by intracardiac injection. By any type of from the administration routes, great amount of the absorbed vitamin B_<12> was distributed in the kidney. Twenty four hours after an intramuscular injection of labelled vitamin B_<12> about 85 percent of it was retained in the body and even 10 days later, 60 percent was still remained. Five days after an oral administration of labelled vitamin B_<12>, the most of it was distributed in the intestinal walls, especially in the upper half of the small intestines. The absorption of vitamin B_<12> orally when given appeared to increase by adding human parotid saliva to it.
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Goichiro KATSUI
Article type: Article
1961 Volume 24 Issue 4 Pages
246-248
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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The chemical reaction of vitamin A aldehyde with antimony trichloride reagent resulted in the formation of isomerized vitamin A aldehyde at first. As the increase in the quantity of adding antimony trichloride reagent as well as in the time of the reaction, the decomposition of vitamin A aldehyde was accerelated towards the formation of a substance of which maximum absorption was detected at 375 and 305-315mμ.
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
248-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
248-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
248-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Eiichi HIRAOKA, Hideaki OMORI
Article type: Article
1961 Volume 24 Issue 4 Pages
249-252
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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It was investigated that N-oxide derivatives of nicotinic acid and its amide showed no vitamin activity on the growth of Lactobacillus arabinosus 17-5 and Staphylococcus aureus 209-P which require nicotinic acid or its amide as essential vitamins. Nicotinic acid or nicotinamide-N-oxide was not antimetabolite of nicotinic acid or its amide for these microorganisms. These N-oxides did not be reduced to nicotinic acid or its amide by L. arabinosus, Staph. aureus, Escherichia coli or bakers'yeast.
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
252-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
252-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Eiichi HIRAOKA, Hideaki OMORI, Keiko MATSUI
Article type: Article
1961 Volume 24 Issue 4 Pages
253-256
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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The growth of Lactobacillus arabinosus or Saccharomyces carlsbergensis was inhibited by INAH-N-oxide added in the medium containing a minimum amount of requisite vitamins. The growth inhibitory effect of INAH-N-oxide was a little weaker than INAH but stronger than Na-glucuronate isonicotinohydrazone. The growth inhibition of INAH-N-oxide was recovered by pyridoxal or pyridoxamine on L. arabinosus and by any of three vitamins of B_6 group on Sacch. carlsbergensis. The activity of tryptophanase of Escherichic coli was inhibited by INAH-N-oxide and the inhibition was recovered by the vitamins of B_6 group. These results indcated that INAH-N-oxide was antimetabolite of vitamin B_6 as INAH and Na-glucuronate isonicotinohydrazone.
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Eiichi HIRAOKA, Hideaki OMORI
Article type: Article
1961 Volume 24 Issue 4 Pages
256-258
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Whether INAH-N-oxide was reduced to INAH or not at the state of growth inhibition on Laciobacillus arabinosus was examined using the paper partition chromatography. It was assumed recently that antibacterial activity of Na-glucuronate isonicotinohydrazone is due to INAH, formed by hydrolysis in the medium. So Na-glucuronate isonicotinohydrazone was examined in the same manner with INAH-N-oxide. In these studies INAH-N-oxide was not reduced to INAH whereas Na-glucoronate isonicotinohydrazone was converted to INAH in the medium by hydrolysis. It was assumed from these results that the antibacterial activity of INAH derivatives is due to that the hydrazide group of INAH is free form or the substituted hydrazide group to form free hydrazide easily.
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
258-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Tadakatsu KATO, Shoichi SHIMIZU
Article type: Article
1961 Volume 24 Issue 4 Pages
259-262
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Some investigations were made on the microbiological activities of cobamides in feed upplement as a sample of fermentation products for determinating natural form of cobamides. An aqueous extract obtained by stirring the sample in water at 30℃ showed almost equal activity in Lactobacillus leichmannii assay to an extract with a boiling potassium cyanide solution. On the other hand, in Ochromonas malhamensis assay using Ford's medium containing no cyanide ion, the aqueous extract showed extremely low activity. However, addition of ascorbate or thioglycolate as stabilizing agents to the medium caused negligible loss of activity. Also, instead of autoclaving, an aseptical addition of the extract to the sterilized medium after passing through germ filter was successfully carried out. It was concluded from these results that low activity of the aqueous extract in usual Ochromonas assay was due to the existence of non-cyano type cobamides which is less stable to heat than cyano type. The non-cyano type cobamides were considered to be comparably active to cyano type and their electrophoretic and other behavior will be described elsewhere.
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
262-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Nobuko OISHI, Saburo FUKUI
Article type: Article
1961 Volume 24 Issue 4 Pages
263-266
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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In a preceding paper a presumption, from the results of growth experiments, chemical assay, and paper ionophoresis of the incubation products, was proposed that a stimulatory action of oxythiamine on yeast would be due to cleavage of the antagonist to its pyrimidine (oxypyrimidine) and thiazole moieties and utilization of the latter as a precursor in thiamine biosynthesis. This paper deals with isolation of oxypyrimidine from culture of Sacch. sake in a grown medium added with oxythiamine in order to ascertain the presumption above mentioned. UV spetrum, melting point, Rf value and elementary analytical data of the white crystal isolated from the incubation mixture using cellulose column chromatography, were identical with those of authentic sample of 2-methyl-4-hydroxy-5-hydroxymethylpyrimidine.
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Tokio KOBAYASHI, Michiko FUKANO, Teijiro KAMIHARA
Article type: Article
1961 Volume 24 Issue 4 Pages
266-269
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Effect of rice germ, rice germ koji, mycelium of Aspergillus oryzae or brewers' yeast on the growth of rats was examined on a basal diet consisting of polished rice, wheat flour, fish meal, soy bean oil and fresh radish leaves which are the staple foodstuffs for Japanese people. Though the average weight gain on the basal diet for 30 days was less than 50g, the growth was slightly stimulated by the rice germ containing diet and the addition of yeast, rice germ koji or mycelium of Aspergillus oryzae caused a remarkable weight gain, namely more than 100g for 30 days. The mycelium or koji cotnaining diet showed a much higher food efficiency than not only the basal diet, but also the germ or yeast diet. The fact suggests that mycelium of Aspergillus oryzae contains some specific growth factor (s).
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Tokio KOBAYASHI, Michiko FUKANO, Teijiro KAMIHARA
Article type: Article
1961 Volume 24 Issue 4 Pages
269-272
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Growth stimulating effect on rats exerted by mycelium of Aspergillus oryzae added to a diet containing the staple foodstuffs for Japanese people was found to be mainly due to vitamins of B group. Among the vitamins, riboflavin was most effective. Consequently, insufficiency of the vitamin in the staple foodstuffs for Japanese people was reperceived. Furthermore, it is likely that the mycelium contains some other growth factor (s) than the known vitamins, since an additional effect of the mycelium to the all vitamins containing diet was observed.
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
272-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
272-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
272-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
272-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
272-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Article type: Appendix
1961 Volume 24 Issue 4 Pages
273-274
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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[in Japanese], [in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
275-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
276-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
276-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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Article type: Appendix
1961 Volume 24 Issue 4 Pages
277-278
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
279-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
279-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
280-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
280-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
280-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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[in Japanese]
Article type: Article
1961 Volume 24 Issue 4 Pages
280-
Published: December 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL
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