VITAMINS
Online ISSN : 2424-080X
Print ISSN : 0006-386X
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Volume 82, Issue 5-6
Displaying 1-14 of 14 articles from this issue
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  • Bactericidal Action of D-arabo-Ascorbate-Iron(II) Complex
    Akira Murata, Yoshinori Higashiho, Mariko Yamauchi, Yuka Kohara, Kimie ...
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 321-327
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_321
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    The in vitro effect of D-arabo-ascorbate-iron(II) complex ([DaA-Fe^<2+>]) on bacteria was investigated using eight bacterial species. [DaA-Fe^<2+>] was bactericidal toward all species examined. The bacteria were sensitive to [DaA-Fe^<2+>] in this order from greatest to least: Bacillus subtilis, Lactobacillus casei, Salmonella typhimurium, Escherichia coli, Staphylococcus aureus, Proteus vulgaris, Micrococcus flavus and Morganella morganii. Mechanism of the bactericidal action of [DaA-Fe^<2+>] was investigated with E. coli, B. subtilis, L. casei and S. aureus. Bubbling of nitrogen gas through the reaction mixture inhibited the action. Addition to the reaction mixture of reducing agents, chelating agents, radical scavengers, catalase and superoxide dismutase inhibited the action. Transition metal ions enhanced the action. The findings indicate that free radical mechanism is involved in the action and reactive oxygen species are responsible for the bactericidal action. In [DaA-Fe^<2+>]-treated bacterial cells, adsorption of T-phages was inhibited, suggesting the alteration of cell surface by [DaA-Fe^<2+>].
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  • Vitamin C Activity of Dehydro-L-Ascorbic acid in ODS Rats [2]
    Shizu Higasa, Masaru Tsujimura
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 329-335
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_329
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    The effects of oral administration of L-ascorbic acid (AsA) and dehydro-L-ascorbic acid (DAsA) on ODS rat development were examined. AsA or DAsA was administered orally at 2mg/0.5mL ion-exchanged water/day. No difference was observed in body weight changes during the observation period between the AsA and DAsA groups. The animals were anatomized on Day 21, and various component analyses were performed. The total vitamin C levels in the brain and liver and serum alkaline phosphatase activity were significantly lower in the DAsA group than in the AsA group (p<0.01). The malondialdehyde concentration, which is an index of serum lipid peroxide level, was significantly higher in the DAsA group than in the AsA group (p<0.05). The hydroxyproline level, which is an index of the collagen content, was measured in the sternum (xiphoid process) and auricle, but no difference was noted between the two groups. No difference was noted in the systolic blood pressure or heart rate on the day before autopsy between the two groups.
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  • Structural change in the activation of inactive recombinant glutamate dehydrogenases from thermophiles/hyperthermophiles produced in Escherichia coli
    Shuichiro Goda, Haruhiko Sakuraba, Yuzuru Hiragi, Toshihisa Ohshima
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 337-343
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_337
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    Glutamate dehydrogenases from thermophiles and hyperthermophiles have been reported to be expressed as inactive forms in a mesophile Escherichia coli and differ in their form from the native enzymes. We have recently found that the recombinant Pyrobaculum islandicum glutamate dehydrogenase (Pis-GDH) expressed in E. coli hardly exhibits the activity, and that the heat treatment of the inactive enzyme at 90℃ or the addition of 5M urea to the inactive enzyme solution dramatically activated to the activity level comparable to that of the native enzyme. Our small-angle X-ray scattering measurements showed that the size of inactive molecule markedly decreases and the arrangement of quaternary structure changes accompanying the activation without change in the molecular weight of the enzyme. This finding indicates that the subunit rearrangement - i.e., a change in the quaternary structure of the recombinant hexameric Pis-GDH - is essential for creation of the active enzyme.
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  • Nampt-mediated, systemic NAD synthesis is necessary for pancreatic β cell function
    Mariko Shin
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 345-348
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_345
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  • A novel ^D-serine dehydratase from Saccharomyces cerevisiae and its application to ^D-serine assay
    Tomokazu Ito, Tohru Yoshimura
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 349-351
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_349
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 353-
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_353_1
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 353-354
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_353_2
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 354-355
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_354
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 355-356
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_355
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  • [title in Japanese]
    [in Japanese]
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 356-357
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_356_2
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 358-
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_358_1
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 358-359
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_358_2
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  • [title in Japanese]
    [in Japanese]
    Article type: Article
    2008 Volume 82 Issue 5-6 Pages 359-
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_359
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  • [title in Japanese]
    Article type: Bibliography
    2008 Volume 82 Issue 5-6 Pages 360-365
    Published: June 25, 2008
    Released on J-STAGE: October 10, 2017
    DOIhttps://doi.org/10.20632/vso.82.5-6_360
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