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Volume 74, Issue 11
Displaying 1-5 of 5 articles from this issue
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Keiko TANAKA, Kazuko YAMADAa, Ruamsub CHUMNANTANA, Makoto ASHIUCHI, To ...Article type: Article
2000 Volume 74 Issue 11 Pages 543-549
Published: November 25, 2000
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSThere are divergent pathways for de novo pyridoxine biosynthesis. Yeasts(fission and budding yeasts) have a different pathway from that of Escherichia coli. We have examined possible precursors in the pathway of yeast. Based on the result that an L-leucine-requiring mutant strain of fission yeast (Scbizosaccharomyces pombe) showed correlation between concentration of L-leucine and productivity of vitamin B_6, we have examined 2-isopropylmaleate and 3-isopropylmaleate, intermediates of L-leucine biosynthesis in the yeast, as the candidate of precursor of pyridoxine biosynthesis. Competitive experiments with [U-^<13>C]D-glucose showed that the two compounds may not be the precursor. 2-Oxoisovalerate, L-valine and L-isoleucine inhibited incorporation of ^<13>C intO pyridoxine in the competitive experiment. Thus, 2-oxoisovalerate or its related compound was suggested to be the candidate of the precursor.View full abstractDownload PDF (552K) -
Mitsuko OKADA, Mayumi SHIBUYA, Eriko YAMAMOTO, Yoko MURAKAMIArticle type: Article
2000 Volume 74 Issue 11 Pages 551-552
Published: November 25, 2000
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (183K) -
[in Japanese]Article type: Article
2000 Volume 74 Issue 11 Pages 553-554
Published: November 25, 2000
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (248K) -
[in Japanese]Article type: Article
2000 Volume 74 Issue 11 Pages 555-557
Published: November 25, 2000
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (301K) -
[in Japanese]Article type: Article
2000 Volume 74 Issue 11 Pages 559-560
Published: November 25, 2000
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (309K)
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