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Volume 85, Issue 7
Displaying 1-9 of 9 articles from this issue
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Shuhei Ebara, Toshiaki WatanabeArticle type: Article
2011 Volume 85 Issue 7 Pages 335-337
Published: July 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSVitamin assays are classified into biological and chemical methods. A microbiological assay in the former class and a high-performance liquid chromatographic assay in the latter are widely used presently. A assay is carried out for the determination of the vitamins in foods or clinical samples. We adopt an appropriate assay depending on the purpose. These assays are not completely settled and remain problems to be solved.View full abstractDownload PDF (509K) -
Yukihisa Ishiwata, Hiromi OkaArticle type: Article
2011 Volume 85 Issue 7 Pages 338-345
Published: July 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSAs for the measurement of the content of vitamin B_1 in foods and biological samples, BrCN-thiochrome fluorescent method was used as a standard method for a long time up to the first half of 1980s. However, the fluorescent detection method by HPLC has become a mainstream nowadays. The measurement of vitamin B_1 by HPLC is classified into two methods, pre-column derivatization method and post-column derivatization method, by the timing of adding the thiochrome (Thc) regent whether it is added before the passage of the HPLC column or after. In the measurement of thiamin in whole blood, the both methods showed fine chromatographic separation with a single peak. The retention times by pre- and post- column derivatization methods were 5.1 minutes and 5.5 minutes, respectively. Regarding the precision, the CV by the two methods were 2.2% and 3.1%, respectively, and therefore the accuracy of the two methods was regarded to be comparable. However, it was proved that the correlation of the two methods was r=0.875, y=0.804x+8.423. Thus, post-column derivatization method tended to show lower value than the other method. Therefore, in the evaluation by HPLC analysis for clinical diagnosis and nutritional surveillance, it would be needed to conduct HPLC by employing the same method, pre- or post-column derivatization method.View full abstractDownload PDF (941K) -
Takashi Tamura, Takahiro OkugochiArticle type: Article
2011 Volume 85 Issue 7 Pages 346-348
Published: July 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (519K) -
Shoji Yamada, Keiko YamadaArticle type: Article
2011 Volume 85 Issue 7 Pages 349-352
Published: July 25, 2011
Released on J-STAGE: October 10, 2017
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Mariko Shin, Haruka MakiArticle type: Article
2011 Volume 85 Issue 7 Pages 353-356
Published: July 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (637K) -
[in Japanese], [in Japanese], Adebanjo A. BadejoArticle type: Article
2011 Volume 85 Issue 7 Pages 357-358
Published: July 25, 2011
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
2011 Volume 85 Issue 7 Pages 358-359
Published: July 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (340K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2011 Volume 85 Issue 7 Pages 359-
Published: July 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (154K) -
Article type: Bibliography
2011 Volume 85 Issue 7 Pages 360-364
Published: July 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (629K)
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