VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 43, Issue 5

Displaying 1-36 of 36 articles from this issue

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Enzymatic Formation of New L-Ascorbic Acid Glycosides (Preliminary Report)

Toshio MIYAKE, Yukio SUZUKI

Article type: Article
1971 Volume 43 Issue 5 Pages 205-209
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_205

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By paper chromatography, two new spots having reducing power on 2,6-dichlorophenol-indophenol and silver nitrate beside the spot of L-ascorbic acid were found in the incubation mixture containing partially purified transglucosidase from Aspergillus niger IAM 2534,maltose, sodium L-ascorbate and thiourea. The new derivatives were also precipitated as lead compounds in diluted alkali solution like as L-ascorbic acid. The lead precipitate was purified by washing with diluted alkali solution, and by repeated process of liberation from lead precipitate at pH 3.0 by sulfuric acid and reprecipitation as lead compounds in diluted alkali solution until no more free sugar was detected on paper chromatogram. Hydrolysis of the purified derivatives in N H_2SO_4 or with the partially purified transglucosidase from Asp. niger gave D-glucose and L-ascorbic acid. D-Glucose was identified by glucose oxidase and by paper chromatography. From these results, two new derivatives enzymatically formed from AsA and maltose are thought to be L-ascorbic acid glucoside-like and its oligosaccharide-like compounds.

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On the Fractional Determination of Ascorbic, Dehyroascorbic, and Diketogulonic Acids.

Masaru TSUJIMURA, Yoshiko UCHIYAMA, Akiji FUJITA

Article type: Article
1971 Volume 43 Issue 5 Pages 210-220
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_210

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The 2,4-dinitrophenylhydrazine method for fractional determination of ascorbic, dehydroascorbic and diketogulonic acids according to Roe was examined and the improved conditions were described : In the presence of oxyhemoglobin, ascorbic acid (AsA) is oxidized to dehydroascorbic acid (DAsA) during deproteinization. For preventing the oxidation of AsA in blood during deproteinization, blood is hemolyzed with water, followed by addition of stannous chloride and metaphosphoric acid. For reduction of DAsA to AsA H_2S is passed through the deprotenized solution containing SnCl_2,adjusted to pH 3 with sodium acetate and the whole is kept at 37℃ for 2 hours. H_2S is expelled by N_2 or CO_2 after addition of thiourea. The solution is filtered and determined, whereby DAsA is practically completely reduced to AsA leaving"diketogulonic acid"as itself. The difference from the total AsA is assumed to be the DAsA. In fresh animal and vegetable tissues the sum of DAsA and diketogulonic acid is small as compared with the total ascorbic acid. In such cases, the determination of"diketogulonic acid"can be practically simplified as follows : The solution deproteinized with 5% metaphosphoric acid without indophenol oxidation is kept at 37℃ for 15 minutes after equilibration with H_2S, followed by addition and dissolution of thiourea and kept further at 37℃ for 20 minutes.

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Metabolism of Vitamins and Trace Element COMPREHENSIVE BIOCHEMISTRY VOL. 21 (1971) Elsevier (Amsterdam)

[in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 220-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_220

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Biological Studies on Pantethine : (IX) ANTILIPIDEMIC EFFECTS IN RATS AND CHICKS

Takeshi ONODERA, Masanao ISHIHARA, Akira KASAHARA

Article type: Article
1971 Volume 43 Issue 5 Pages 221-225
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_221

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Effects of pantethine (PaSS) on serum levels of lipids of rats and chicks have been examined : PaSS, when administered orally in a daily dose of 1g/kg for 10 days, decreased serum cholesterol levels of the rats fed on normal diet. One-week administrations of PaSS inhibited the increase of serum cholesterol levels produced by feeding rats on high-cholesterol diet, while the effect was not clear in case of two- and three-week administrations of the compound. In chicks fed on high-cholesterol diet, PaSS was found to suppress the increase of serum levels of phospholipid and triglyceride as well as that of cholesterol. In addition, suppression of pathological changes in the aorta and liver of the animals has also been observed by administration of PaSS.

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Studies on the Distribution of Pantothenic Acid, Coenzyme A and their Intermediates in Rat Liver. : (IV) PRELIMINARY EXPERIMENT ON THE RELATIONSHIP BETWEEN ACYL CARRIER PROTEIN AND THE INTERMEDIATES OF PANTOTHENIC ACID METABOLISM.

Tsuneo NAKAMURA, Tomoichi KUSUNOKI, Kokichi SOYAMA

Article type: Article
1971 Volume 43 Issue 5 Pages 226-230
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_226

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From the results of experiment on acyl carrier protein of Escherichia coli origin added to the rat liver sample, it was presumed that the similar type of acyl carrier protein was not proved in the rat liver. However, CoA content in rat liver supernatant provided by homogenization with 3% perchloric acid solution was estimated up to larger amount than that in similar supernatant produced by extraction with boiling water. On the contrary the total pantothenic acid level in the former sample was remarkably lower than in the latter. Moreover, in the precipitate produced by the treatment with perchloric acid, pantothenic acid was demonstrated after mild hydrolysis by alkaline. From above mentioned results, the posibility of the existence of acyl carrier protein-like substance in rat liver was concluded.

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Percutaneous Resorption of Tocopheryl Nicotinate

Mitsuo KAMIMURA

Article type: Article
1971 Volume 43 Issue 5 Pages 231-236
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_231

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Percutaneous resorption of α-tocopheryl nicotinate (Toc-nic) was observed by means of the fluorescent microscopy, microautoradiography and analysis of the vitamin E. And the following observation has been acquired and some discussions have been made. i) Toc-nic is absorbed well through the healthy hartless human skin and scar. There are two routes of absorption through the horny layer, the epidermis and the borderline between the epidermis and dermis, additioned through the pilocebaceous canal and hair sheaths. ii) The material has been well absorbed in dermis, and at the same time, a part of it is changed to the free tocopherol in a few hours. iii) The material has been proven to have a microdistribution to the many systems and especially a high affinity for small blood vessels everywhere like as tocopheryl acetate. iv) The material has been in the sebaceous glandulae, especialy intracellular region of sebaceous cells, and may be the form of the Toc-nic. v) It has been suggested that the topical effects of the Toc-nic may exist.

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Effect of Dietary Composition on Vitamin B_6 Deficiency State. : (I) ON THE DIETARY PROTEIN LEVELS AND LIVER TRANSAMINASE ACTIVITIES IN RATS.

Mitsuko OKADA, Atsuko OCHI

Article type: Article
1971 Volume 43 Issue 5 Pages 237-240
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_237

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Effect of vitamin B_6 was investigated on rats fed on diet containing different levels of protein. The growth of rats on 70% casein diet was retarded in a week after the vitamin B_6 depletion, while that of rats received 10% casein diet was normal during 4 weeks period regardless of diet with or without vitamin B_6. Liver transaminase activities (alanine, aspartate and ornithine transaminases) of rats were determined. Rats on B_6-depleted high protein diet were decreased in all liver transaminase activities measured and especially soluble alanine transaminase was most labile on the vitamin B_6 depletion. Soluble aspartate transaminase which is known to be induced by high protein administration was low in the livers of rats on B_6-depleted high protein diet rather than in that on low protein diet. Ornithine transaminase activity was found to be largely decreased in terms of holo-enzyme.

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Effect of Dietary Composition on Vitamin B_6 Deficiency State. : (II) FATTY LIVER INDUCED BY HIGH PROTEIN DIET WITHOUT B_6

Mitsuko OKADA, ATSUKO OCHI

Article type: Article
1971 Volume 43 Issue 5 Pages 241-244
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_241

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Effect of vitamin B_6 depletion on the hepatic compositions of rats fed 70% or 10% casein diet was investegated. Lipid accumulation was observed in the livers of rats 70% casein diet without B_6. The lipid accumulated was twice as high as that of control rats supplemented with B_6 and mainly consisted of glyceride and cholesterol, whereas phospholipid content was unchanged. The lipid accumulation was histologically defined as a fatty degeneration with central globules. Liver protein content was maintained almost at a constant level under any conditions studied.

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A New Fat-soluble Pantothenic Ester and its Biological Activity on Rats (Preliminary Report)

Yukio SHIBATA, Sumiko YONEI, Kiyoe OHIRA, Michiko SUGANUMA, Hiroko TSU ...

Article type: Article
1971 Volume 43 Issue 5 Pages 245-248
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_245

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Diethoxycarbonyl-pantothenic ethyl ester (DEPaE) was prepared by Fujimoto, as a new fat-soluble ester of pantothenic acid (PaA), stabler than salts of PaA and its biological activity was compared with equimolar Ca salt of PaA (CaPa) on rats : DEPaE, when orally given to young rats on PaA-deficient diet, was proved to be fully active as PaA in both the weight gain and the increase of CoA level of liver. Activity of both phenylalanine-4-hydroxylase and L-tyrosine 2-oxoglutarate aminotransferase of liver was shown to be higher in DEPaE and CaPa groups than in deficient group. DEPaE is easily converted into PaA by mild alkaline hydrolysis and from our preliminary experiment, DEPaE was fully utilized in animals as PaA.

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 249-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_249_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 249-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_249_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 249-250
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_249_3

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 250-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_250_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 250-251
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_250_2

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[title in Japanese]

[in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 252-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_252_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 252-253
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_252_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 253-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_253_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 253-254
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_253_2

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[title in Japanese]

[in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 254-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_254_1

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[title in Japanese]

[in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 254-255
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_254_2

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[title in Japanese]

[in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 255-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_255

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 256-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_256_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 256-257
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_256_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 257-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_257_1

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[title in Japanese]

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Article type: Article
1971 Volume 43 Issue 5 Pages 257-258
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_257_2

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[title in Japanese]

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Article type: Article
1971 Volume 43 Issue 5 Pages 258-259
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_258

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 259-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_259_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 259-260
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_259_2

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[title in Japanese]

[in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 260-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_260_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 260-261
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_260_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 261-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_261_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 261-262
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_261_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 262-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_262_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 262-263
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_262_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 263-
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_263_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1971 Volume 43 Issue 5 Pages 263-264
Published: May 25, 1971
Released on J-STAGE: February 27, 2018

DOIhttps://doi.org/10.20632/vso.43.5_263_2

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