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Volume 45 (1972)
- Issue 6 Pages 317-
- Issue 5 Pages 233-
- Issue 4 Pages 175-
- Issue 3 Pages 113-
- Issue 2 Pages 69-
- Issue 1 Pages 1-
Volume 45, Issue 2
Displaying 1-13 of 13 articles from this issue
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Kiyoshi HARADA, Keiichi KOHNO, Isamu DAIRA, Isamu UTSUMIArticle type: Article
1972 Volume 45 Issue 2 Pages 69-75
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESS3'-or 5'-Substituted pyridoxal (PAL) derivatives were parenterally administered into rabbits and rats (10mg/kg) and the resultant B_6 levels in the blood and liver were determined. Among seven compounds tested, PAL-5'-n-butyrate, PAL 5'-isobutyrate and PAL 5'-benzoate gave higher peak levels with larger biological half lives of blood total PAL. On the intraveneous administration of these three compounds, the elimination process were specifically followed by a 2-phased first order kinetics though the reason of which was unexplained. Less lipophilic 5'-substitutes, PAL 5'-acetate, PAL 3', 4'-diacetate, PAL 5'-succinate and PAL 3'-n-butyrate gave similar B_6 levels to that of PAL administration. PAL 5'-acylates were confirmed to be hydrolyzed in the blood, mainly by a kind of plasma esterase. The esterase activity was somewhat different with animal species and the kind of substitutes. From these findings effects of 5'-acylation on the biopharmaceutical behavior of PAL are discussed.View full abstractDownload PDF (571K) -
Kiyoshi HARADA, Keiichi KOHNO, Isamu DAIRA, Isamu UTSUMIArticle type: Article
1972 Volume 45 Issue 2 Pages 76-80
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSChanges in the blood B_6 levels after oral administration of pyridoxal 5'-n-butyrate (PAL・NB), PAL 5'-isobutyrate (PAL・IB), PAL 5'-benzoate (PAL・BE), PAL 3'-n-butyrate, PAL 3', 4'-diacetate, PAL 5'-acetate and PAL 5'-succinate to rat were followed by fluorometric procedure. The maximum total PAL concentrations were mostly the same as in the case of PAL administration, but higher levels were maintained by PAL 5'-acylates. In the case of PAL・NB, ・IB and ・BE, the areas under the time-blood concentration curve (AUC) were 1.5 times larger than that of PAL administration. Possible hydrolysis of the 5'-acylates in the intestine was suggested in circulation experiments using rat intestine in situ and experiments with homogenate of the intestine in vitro. On the other hand, absorption of the 5'-acylates in their intact forms was also evidenced by fractional determination as well as bioautography using Sacch. carlsbergensis of the blood after administration of PAL・BE and ・NB. In this respect, these 5'-acylates were different from the reported pyridoxine acylate with longer chains. From these results, characteristics of 5'-acylation of PAL molecule are discussed on the view point of drug latentiation and prolongation.View full abstractDownload PDF (549K) -
Mitsunori HAYASHI, Tadashi KAMIKUBOArticle type: Article
1972 Volume 45 Issue 2 Pages 81-85
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSThe biosynthesis of corrinoids by the two mutants of R. spheroides, PM-8 and PM-8 : bg 58,unable to grow photosynthetically, was studied. Corrinoids were extracted from the cells grown aerobically in the dark. Microbiological activities of the extracts from the strains PM-8 and PM-8 : bg 58 for Ochromonas malhamensis were 83% and 92% of those for Escherichia coli, respectively. Paper-ionophoretic and spectrophotometric investigation of the purified corrinoids showed that cobalamin was the major component of the corrinoids in the both strains. The yield of cobalamin coenzyme in PM-8 was 16μg per gram of dry cells. Thus, the formation of corrinoids in the two mutant strains seems to be similarto that in the wild strain of R. spheroides. The accumulation of coproporphyrin was stimulated by the addition of δ-aminolevulinic acid to the medium, while the corrinoid formation was not affected. The finding suggests that corrinoid formation might be restricted under the condition tested.View full abstractDownload PDF (469K) -
Yukio SUZUKIArticle type: Article
1972 Volume 45 Issue 2 Pages 86-89
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSBoth Candida albicans and C. tropicalis produced a remarkable amount of riboflavin-α-glucoside in growing cultures, when grown on a medium containing dextrin and riboflavin. Moreover, it was pointed out that a partially purified enzyme preparation of transglucosyl-amylase prepared by the method of Sawai and Hehre had riboflavin-α-glucoside-forming activity. Maltosyl compounds such as maltose, dextrin and amylose were the effective glucosyl donor, whereas glucose, fructose, sucrose, lactose, trehalose and dextran were inactive.View full abstractDownload PDF (477K) -
Tsuneyuki OKU, Junichi MATSUURA, Norimasa HOSOYAArticle type: Article
1972 Volume 45 Issue 2 Pages 90-97
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSThe binding proteins of vitamin D_3 and 25-hydroxycholecalciferol were identified in rat plasma. Five protein peaks (designated as peak 1,2,3,4 and 5 according to the elution order) were observed on the gel filtration of Sephadex G-200 (2.4×65cm). Peak 1,2 and 4 bound with vitamin D_3 and 25-hydroxycholecalciferol. However, peak 2 and 4 bound the considerable amount of vitamin D_3 and peak 4 bound with 25-hydroxycholecalciferol. The properties of these peak fractions were also analyzed with the polyacrylamide gel disc electrophoresis and the sucrose density gradient ultracentrifugation. Vitamin D_3 bound with more than five bands of gel stained with acid blue black 10 B. Four bands of them contained a small amount of carbohydrate and lipid, and a band of them was α-globulin. The α-globulin band associated specifically with 25-hydroxycholecalciferol. The sedimentation constant of vitamin D_3 binding protein was calculated as 14.5 s, 10.5 s, 8.1 s, 6.2 s and 4.3 s, and the 25-hydroxycholecalciferol was as 14.5 s, 10.5 s, 6.8 s and 4.3 s. The sedimentation constant of the protein, to which vitamin D_3 bound specifically, was calculated as about 8.1 s. The protein for 25-hydroxycholecalciferol was 6.8 s.View full abstractDownload PDF (745K) -
Masakazu OHTSUKIArticle type: Article
1972 Volume 45 Issue 2 Pages 98-103
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSThiamine phosphate pyrophosphorylase (EC 2.5.1.3) has been isolated from cell-free extracts of E. coli PT-R1 (pyrithiamine resistant mutant) and partially purified approximately 30-fold with regard to the specific activity. It was demonstrated that the enzyme catalyzes the synthesis of thiamine monophosphate from hydroxymethylpyrimidine pyrophosphate and hydroxyethylthiazole monophosphate in the same manner as the yeast enzyme. It was labile to heating and required Mg^<2+> for optimal activity. The optimal pH and temperature were estimated at 9.0 and 37℃ respectively. The reaction was inhibited by PCMB. The enzyme was also inhibited by ATP of which concentration showing 50% inhibition was 2.4×10^<-3>M.View full abstractDownload PDF (500K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1972 Volume 45 Issue 2 Pages 104-
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSDownload PDF (135K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1972 Volume 45 Issue 2 Pages 104-105
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSDownload PDF (277K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1972 Volume 45 Issue 2 Pages 105-
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSDownload PDF (188K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1972 Volume 45 Issue 2 Pages 105-
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSDownload PDF (188K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1972 Volume 45 Issue 2 Pages 106-
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSDownload PDF (186K) -
[in Japanese], [in Japanese]Article type: Article
1972 Volume 45 Issue 2 Pages 106-107
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSDownload PDF (308K) -
[in Japanese]Article type: Article
1972 Volume 45 Issue 2 Pages 107-
Published: February 25, 1972
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSDownload PDF (171K)
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