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Volume 50, Issue 9-10
Displaying 1-23 of 23 articles from this issue
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Luigi M. DELUCA, Yasutoshi MUTOArticle type: Article
1976 Volume 50 Issue 9-10 Pages 345-349
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
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Atsuko ADACHI, Tadashi KOBAYASHIArticle type: Article
1976 Volume 50 Issue 9-10 Pages 351-357
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSMethods for the gas-liquid chromatographic (GLC) determination of vitamin D in multivitamin preparations as described in the previous reports^<1), 2)> were applied to various kinds of commercial samples. The first method^<1)> including separation of the unsaponifiable matters, thin-layer chromatography (TLC) and GLC was applied to usual samples, while the second method^<2)> including separation of the unsaponifiable matters, phosphate-treated alumina column chromatography^<5)>, TLC and GLC was applied to the samples containing excess amounts of vitamin E (more than 2,500 of the weight ratio of DL-α-tocopheryl acetate to vitamin D). When vitamin D contents in 6 kinds of commercial samples were repeatedly estimated by either the proposed first or second method, good reproducibility was obtained in all cases. The methods were also applied to 3 kinds of commercial samples stored for about 1 year at either room temperature or 40℃. Although the samples contained comparatively large amounts of decomposed products of vitamin D, A and others formed during storage, the pyrovitamin D peaks were not disturbed by decomposed products on the gas chromatograms. From these results, the proposed methods were confirmed to be suitable routine for the determination of vitamin D in commercial multivitamin preparations.View full abstractDownload PDF (692K) -
Atsuko NISHINOArticle type: Article
1976 Volume 50 Issue 9-10 Pages 359-364
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSThiamine-binding protein of E.coli KG33,auxotrophic for thiamine thiazole was purified to homogeneity by ammonium sulfate treatment, negative DEAE-cellulose chromatography, and affinity chromatography using agarose coupled with thiamine diphosphate. Purified thiamine-binding protein has a molecular weight of 38,000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Although 8M urea reversibly inhibits the binding of thiamine to thiamine-binding protein, no evidence was obtained for subunit formation of the protein in the presence of urea.View full abstractDownload PDF (651K) -
Atsuko NISHINOArticle type: Article
1976 Volume 50 Issue 9-10 Pages 365-370
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSBinding of thiamine to thiamine-binding protein is reversible, with a dissociation constant for the thiamine-binding protein-thiamine complex of about 9nM. The optimal pH for the binding is between pH 8 to 9. Thiamine analogues such as thiamine monophosphate and chloroethylthiamine competitively inhibit the binding with a Ki value of 3.4×10^<-9>M and 6.8×10^<-7>M respectively. Among various modifying reagents for protein testeld, dimethyl (2-hydroxy-5-nitrobenzyl)-sulfonium bromide which is considered to modify tryptophan residue of the protein, effectively inhibited both the binding of thiamine to thiamine-binding protein and thiamine uptake by whole cells suggesting that tryptophan is involved in the thiamine binding site of thiamine-binding protein in thiamine transport system of E.coli,View full abstractDownload PDF (569K) -
Fumio UEDA, Yoshikazu AYUKAWAArticle type: Article
1976 Volume 50 Issue 9-10 Pages 371-375
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSThin-layer gel filtration of solubilized vitamin A palmitate solution formed with polysorbate 80 has been studied. Thin-layer gel filtration was performed on 0.8 mm thick layer of Sephadex G-200 using water as developing solvent. After development, two spots were detected on a paper print of the gel layer by spraying with Dragendorff reagent. The upper spot was polysorbate 80 micelle containing vitamin A palmitate and the lower seemed to be minor impurities in polysorbate 80. R, value of the upper spot increased with the increasing of the ratio of vitamin A palmitate to polysorbate 80 in solubilized solution. Different behavior of polysorbate 80 micelle containing vitamin A palmitate was not observed by using 0.2 or 2% polysorbate 80 solution as the developing solvent.View full abstractDownload PDF (526K) -
Heitaroh IWATA, Toshio MATSUDA, Satoru YAMAGAMI, Norikazu UEDA, Akemic ...Article type: Article
1976 Volume 50 Issue 9-10 Pages 377-384
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSEffects of thiamine (B_1) administration on glucose intolerance and bradycardia of B_1-deficient rats were investigated. Glucose intolerance was cured by B_1-HNO_3 administration of higher dose than 0.05 mg/kg, when a B_1-deficient diet containing all vitamins other than B_1 was further given after the B_1-administration. The effect of B_1 on glucose intolerance was significantly diminished when a all vitamins free diet in place of B_1-deficient diet was used after the B_1-administration. On the other hand, the restoration of bradycardia by B_1 was observed when either B_1-deficient diet or all vitamins free diet was given after B_1-administration. In addition, when pyridoxine・HC1 (0.2 mg/kg) and hydroxycobalamin acetate (0.002 mg/kg) were simultaneously added to B_1 (0.02 mg/kg) injection, the restorative effect on glucose intolerance but not on bradycardia were seen. These results suggest that bradycardia is a specific sign for B_1-deficiency in the rat but glucose intolerance is resulted from a nutritional disturbance in which vitamins other besides B_1 are also lacking.View full abstractDownload PDF (662K) -
[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 385-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
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[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 385-386
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
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[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 386-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (182K) -
[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 386-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (182K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 387-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 387-388
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
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J.Elsas LArticle type: Article
1976 Volume 50 Issue 9-10 Pages 388-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 389-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
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H.N. BHAGAVAN, D.B. COURSINArticle type: Article
1976 Volume 50 Issue 9-10 Pages 389-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
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[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 389-390
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (280K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1976 Volume 50 Issue 9-10 Pages 390-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (181K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1976 Volume 50 Issue 9-10 Pages 390-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (181K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 390-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (181K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 391-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (172K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1976 Volume 50 Issue 9-10 Pages 391-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (172K) -
[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 391-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (172K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 9-10 Pages 391-
Published: October 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (172K)
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