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Volume 81, Issue 5-6
Displaying 1-33 of 33 articles from this issue
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[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 211-212
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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Yasukazu Yoshida, Etsuo NikiArticle type: Article
2007 Volume 81 Issue 5-6 Pages 213-221
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSLipid peroxidation products have received a great deal of attention for the practical application to clinical use as bio-markers. F2-isoprostanes from arachidonates and neuroprostanes from docosahexanoates have been proposed as bio-markers. Although these markers are formed by a free radical-mediated oxidation, the yields from the parent lipids are minimal. In contrast, hydroperoxyoctadecadienoates from linoleates and oxysterols from cholesterols are yielded by much simpler mechanisms from more abundant parent lipids in vivo. We proposed the method in which both free and ester forms of hydroperoxides and ketones as well as hydroxides of linoleic acid and cholesterol are measured as total hydroxyoctadecadienoic acid (tHODE) and 7-hydroxycholesterol (t7-OHCh), respectively. The concentrations of tHODE and t7-OHCh in physiological samples were much higher than that of 8-iso-prostagrandin F_<2α>. In addition to this advantage, hydrogendonor activity of antioxidants in vivo could be determined by the stereoisomeric-ratio of HODE.View full abstractDownload PDF (866K) -
Analysis of Quinonoid Intermediate Formation of Serine Palmitoyltransferase Using Substrate AnalogueHiroko Ikushiro, Shigeru Fujii, Hideyuki HayashiArticle type: Article
2007 Volume 81 Issue 5-6 Pages 223-234
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSSerine palmitoyltransferase (SPT) is a key enzyme of sphingolipid biosynthesis and catalyzes the pyridoxal 5'-phosphate (PLP)-dependent decarboxylative condensation reaction of L-serine with palmitoyl-CoA to generate 3-ketodihydrosphingosine. The binding of L-serine alone to SPT leads to the formation of the external aldimine. The further addition of a non-reactive palmitoyl-CoA analogue caused the apparent accumulation of the quinonoid. NMR studies showed that the hydrogen-deuterium exchange of the α-proton of L-serine was 100-fold accelerated by the binding of the palmitoyl-CoA analogue, showing a remarkable substrate synergism in SPT. Structural model simulations on SPT suggest that the binding of palmitoyl-CoA to SPT induced a conformational change in the PLP-L-serine external aldimine, and that the alternative interactions of His 159 with the two substrates plays an important role in it. This is the unique mechanism of SPT that prevents the formation of the reactive intermediate before the binding of the second substrate.View full abstractDownload PDF (1306K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 235-237
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 238-240
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (344K) -
[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 241-244
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 245-247
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 248-250
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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Article type: Bibliography
2007 Volume 81 Issue 5-6 Pages 251-255
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2007 Volume 81 Issue 5-6 Pages 257-
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (199K) -
[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 257-258
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (364K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 258-259
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 259-260
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (404K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2007 Volume 81 Issue 5-6 Pages 260-
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 260-261
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 261-262
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 262-263
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 263-
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 264-265
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 265-
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2007 Volume 81 Issue 5-6 Pages 265-266
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 266-267
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 267-268
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 269-
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (106K) -
[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 270-
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (199K) -
[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 271-
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 271-272
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (378K) -
[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 272-273
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (386K) -
[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 273-275
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 275-276
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 276-278
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 278-
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (216K) -
[in Japanese]Article type: Article
2007 Volume 81 Issue 5-6 Pages 279-
Published: June 25, 2007
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (57K)
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