VITAMINS
Online ISSN : 2424-080X
Print ISSN : 0006-386X
Search
Advanced search
OR
Browse
Search
Volume 46, Issue 3
Displaying 1-22 of 22 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
  • Studies on the Regulatory Mechanisms for a Biotin-enzyme
    Shosaku NUMA
    Article type: Article
    1972 Volume 46 Issue 3 Pages 85-96
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_85
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Acetyl coenzyme A carboxylase, a biotin-containing enzyme, plays a critical role in the control of fatty acid biosynthesis. Evidence has been presented which indicates that the regulation at this carboxylation step is achieved both by changes in the catalytic efficiency of carboxylase molecules and by changes in the quantity of the enzyme. (1) Kinetic and other studies with the purified liver carboxylase have shown that the main role of the allosteric activator, citrate, in the carboxylase catalysis is to keep the carboxybiotinyl enzyme in active conformation by shifting the equilibrium between the active and inactive species of the carboxylated enzyme. (2) The mechanism by which the carboxylase content of the liver is regulated in various metabolic states has beeh investigated by combined immunochemical and isotopic techniques. The results of these studies indicate that under steady-state conditions the carboxylase content is controlled mainly by changes in the rate of enzyme synthesis, while under nonsteady-state conditions both the rate of enzyme synthesis and the rate constant of enzyme degradation are altered to change the carboxylase content. (Received July 18,1972)
    View full abstract
    Download PDF (1172K)
  • Biological and Coenzymic activity of Vitamin B_6 derivatives : (II) CHEMICAL AND BIOLOGICAL PROPERTIES OF 3-AMINOPYRIDOXAL
    Tatsuo OZAWA, Mitsuaki SANO, Isao TOMITA
    Article type: Article
    1972 Volume 46 Issue 3 Pages 97-103
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_97
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    2-Methyl-3-amino-4,5-dihydroxymethylpyridine, 3-aminopyridoxol (I) is a potent antagonist of pyridoxol in the growth of S. carlsbergensis. Mn0_2 oxidation of (I) gave an aldehyde, 3-aminopyridoxal (II) of mp. 214〜215℃. UV, NMR, and mass spectral data of (II) were compared with those of pyridoxal. It was found that (II) existed mainly as the hemiacetal form (III) in aqueous solution and (II)/(III) was largest at pH 8.3-10.3. (II) was less reactive than pyridoxal with aromatic amine and gave Schiff's base only under heating. (II) inhibited the growth of S. carlsbergensis, though it was weaker than its parent compound (I). (Received May 25,1972)
    View full abstract
    Download PDF (603K)
  • [title in Japanese]
    [in Japanese]
    Article type: Article
    1972 Volume 46 Issue 3 Pages 104-
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_104_1
    JOURNAL FREE ACCESS
    Download PDF (156K)
  • [title in Japanese]
    [in Japanese]
    Article type: Article
    1972 Volume 46 Issue 3 Pages 104-
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_104_2
    JOURNAL FREE ACCESS
    Download PDF (156K)
  • Formation of Riboflavin-β-Galactoside by Crystalline β-Galactosidase from Escherichia coli (Part 2)
    Yukio SUZUKI, Kei UCHIDA
    Article type: Article
    1972 Volume 46 Issue 3 Pages 105-109
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_105
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    It was found that riboflavin-β-galactoside was formed from lactose (or ο- nitrophenyl-β-D-galactopyranoside, ο-NPGa) and riboflavin by crystalline β-galactosidase from Escherichia coli. It was also pointed out in crystalline β-galactosidase that both β-galactosidase activity to hydrolyze ο-NPGa and galactosyltransferase activity to synthesize riboflavin-β-galactoside from ο-NPGa and riboflavin were parallel on some properties such as optimal pH, optimal temperature, pH stability and heat stability. (Received June 2,1972)
    View full abstract
    Download PDF (440K)
  • Hemolysis in Vitamin E-Deficient Erythrocytes Induced by Inorganic Reagents
    Tokuko TATSUTA
    Article type: Article
    1972 Volume 46 Issue 3 Pages 111-116
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_111
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    It has been observed that H_20_2-induced hemolysis ratio is very dependent on the temperature of reaction mixture and is not very correlative with the content of vitamin E in the blood. In order to find more available reagents than H_20_2,firstly various inorganic reagents were tested to see wheather they specifically hemolyze vitamin E-deficient erythrocyte, and then following facts were observed. KI0_3. NaI0_4,Se0_2,FeS0_4,Mo03,CoS0_4 and Ag_2S0_4 were effective under certain condition. However Se02' FeS04' M003' CoS04 and Ag2S04 Were practically unavailable, whereas KI0_3 and NaI0_4 were seemed to be available and the mutual correlation were observed between the hemolysis ratio induced by these reagents and the amount of vitamin E in the serum of new born infant : the correlation coefficients were -0.62 and -0.75 respectively, which values were larger than that between H_20_2 and vitamin E, -0.44. Then, the hemolysis test using KI0_3,NaI0_4 is expected to be useful as a simple biological assay procedure of vitamin E in man. (Received June 3,1972)
    View full abstract
    Download PDF (531K)
  • Affinity of γ-Oryzanol to the Human Skin
    Mitsuo KAMIMURA, Shugo MORITA
    Article type: Article
    1972 Volume 46 Issue 3 Pages 117-119
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_117
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Water soluble γ-oryzanol (Ory) has so strong affinity to the human skin that it is easily adhered to the skin and hardly exfoliated by water and cleanser. The quantity of adhered Ory is fairly proportional to the concentration of dissolved Ory in water (0.1〜1.0%). (Received June 7,1972)
    View full abstract
    Download PDF (321K)
  • Studies on Ergothioneine : (I) DISTRIBUTION IN THE BODY AND THE SUBCELLULAR FRACTIONS OF LIVER
    Takao HAMA, Takao KONISHI, Nanaya TAMAKI, Fusako TUNEMORI, Hajime OKUM ...
    Article type: Article
    1972 Volume 46 Issue 3 Pages 121-126
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_121
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    A new chemical method of assaying ergothioneine (Ergo), was established by taking advantage of its nature that allows to react with p-chloromercuribenzoate but not with N-ethylmaleimide.This method enabled the determinaition of Ergo content in various organs, and revealed that Ergo was contained at high concentrations in the male sex organs. ^3H-Labeled Ergo, when given to rats, was proved to be remarkably incorporated into the male sex organs on the examination of the tissue distribution of 3H. This fact suggested a close relationship between Ergo and sexual organs. Moreover, when examined on the subcellular distribution in the liver, it became clear that this substance resides mostly in the supernatant fraction. (Received June 29,1972)
    View full abstract
    Download PDF (523K)
  • Studies on Ergothioneine : (II) ORIGIN OF ERGOTHIONEINE IN THE ANIMAL ORGANS
    Takao KONISHI, Nanaya TAMAKI, Fusako TUNEMORI, Nobuo MASUMITU, Hajime ...
    Article type: Article
    1972 Volume 46 Issue 3 Pages 127-130
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_127
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    In the previous paper, the authors suggested that ergothioneine (Ergo) is closely related to the sex organs, Ergo found in the animal body was considered to have been derived food source, but the probable participation of the intestinal bacterioflora in its biosynthesis remains to be solved. Accordingly, Ergo content in various organs was compared both in germfree and in conventional rats or mice. As a result, it became clear that Ergo content in organs of germfree animals is higher than that in the corresponding organs of conventional animals, except rat liver, and that the intestinal bacterioflora is rather responsible for decomposition rather than biosynthesis of Ergo. The difference in Ergo concentration was the most remarkable in the male sex organs. In the process of hatch, Ergo content in the whole fertilized egg was unchanged in contrast to the increased content in early stage of embryo, and then diminished. These results suggest that biosynthesis of Ergo in animal body is quite unlikely. (Received June 29,1972)
    View full abstract
    Download PDF (352K)
  • Studies on Ergothioneine : (III) RELATION BETWEEN ERGOTHIONEINE AND ANDROGEN
    Takao KONISHI, Hajime OKUMURA, Nanaya TAMAKI, Takao HAMA
    Article type: Article
    1972 Volume 46 Issue 3 Pages 131-136
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_131
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The relationship between ergothioneine (Ergo) and androgens was studied on the basis of our previous postulation that Ergo derived from food source, may have some effects on the male sex organs. The uptake of ^<14>C-cholesterol, as a steroid model, into the slice of rat seminal vesicle in vitro was doubled when Ergo was present. There was no influence of Ergo on ejaculate volume and fructose content in semen, but androgen showed an effect to control the concentration of Ergo in semen. These results suggested that Ergo, as a common ingredient in semen, exerts some elaborative effects on spermatozoa. (Received June 29,1972)
    View full abstract
    Download PDF (491K)
  • The Uptake of L-Ascorbic Acid and D-Isoascorbic Acid by Mammalian Erythrocytes
    Masato OKAMURA
    Article type: Article
    1972 Volume 46 Issue 3 Pages 137-142
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_137
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The uptake of L-ascorbic acid (L-AsA), D-isoascorbic acid (D-IAsA) and their oxidized forms (L-DAsA. D-DIAsA) by human, monkey, guinea pig, rabbit, sheep and horse erythrocytes in vitro were investigated. Reduced forms of AsA and IAsA are uptaken very slowly for a long time by human, monkey, guinea pig and rabbit erythrocytes ; AsA is uptaken respectively at the rate of 9,9,9,6 % and IAsA is 14,15,21,12 % per two hours. But sheep and horse erythrocytes do not uptake both ASA as well as IAsA. Next, oxidized forms, very different results are got by different species ; that is, oxidized forms are uptaken very rapidly by human and monkey erythrocytes both DAsA and DIAsA, and got in a state of equilibrium within 5〜10 minutes (human erythrocytes uptake 50 % (DAsA), 42 % (DIAsA), monkey 73 % (DAsA), 44 % (DIAsA) after ten minutes) . The great part of uptaken DAsA and DIAsA is reduced (73〜78 %) in the erythrocytes. DAsA is uptaken into guinea pig erythrocytes (19%) as well as in the case of human, monkey, while DIAsA is not uptaken. Then neither DAsA nor DIAsA is uptaken by rabbit, sheep or horse erythrocytes. (Received July 7,1972)
    View full abstract
    Download PDF (541K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1972 Volume 46 Issue 3 Pages 143-
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_143_1
    JOURNAL FREE ACCESS
    Download PDF (173K)
  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1972 Volume 46 Issue 3 Pages 143-144
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_143_2
    JOURNAL FREE ACCESS
    Download PDF (309K)
  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1972 Volume 46 Issue 3 Pages 144-
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_144_1
    JOURNAL FREE ACCESS
    Download PDF (188K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    Article type: Article
    1972 Volume 46 Issue 3 Pages 144-145
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_144_2
    JOURNAL FREE ACCESS
    Download PDF (324K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    Article type: Article
    1972 Volume 46 Issue 3 Pages 145-146
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_145
    JOURNAL FREE ACCESS
    Download PDF (288K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1972 Volume 46 Issue 3 Pages 146-
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_146
    JOURNAL FREE ACCESS
    Download PDF (147K)
  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1972 Volume 46 Issue 3 Pages 147-
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_147_1
    JOURNAL FREE ACCESS
    Download PDF (166K)
  • [title in Japanese]
    [in Japanese]
    Article type: Article
    1972 Volume 46 Issue 3 Pages 147-148
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_147_2
    JOURNAL FREE ACCESS
    Download PDF (298K)
  • [title in Japanese]
    [in Japanese]
    Article type: Article
    1972 Volume 46 Issue 3 Pages 148-
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_148_1
    JOURNAL FREE ACCESS
    Download PDF (183K)
  • [title in Japanese]
    [in Japanese]
    Article type: Article
    1972 Volume 46 Issue 3 Pages 148-149
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_148_2
    JOURNAL FREE ACCESS
    Download PDF (322K)
  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1972 Volume 46 Issue 3 Pages 149-150
    Published: September 25, 1972
    Released on J-STAGE: February 27, 2018
    DOIhttps://doi.org/10.20632/vso.46.3_149
    JOURNAL FREE ACCESS
    Download PDF (222K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top