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Volume 84, Issue 2
Displaying 1-28 of 28 articles from this issue
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[in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 35-36
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (370K) -
Hisashi Muramatsu, Sakuko Ueshima, Hiroaki Yamamoto, Shin-ichiro Kato, ...Article type: Article
2010 Volume 84 Issue 2 Pages 37-45
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSThe reg-psald gene is located immediately upstream of the psald gene encoding phenylserine aldolase in Pseudomonas putida 24-1. The amino acid sequence of Reg-psald showed a high homology with that of AraC family proteins. The Pfam database indicated that Reg-psald contained an AraC-like ligand binding domain in the N-terminal region and two bacterial regulatory helix-turn-helix domains in the C-terminal region. A DNA fragment containing reg-psald and psald genes was ligated into the EcoRI-HindIII site of pUC18. A crude extract of Escherichia coli JM109 cells harboring the resultant plasmid cultured in LB medium containing DL-threo-β-phenylserine showed phenylserine aldorase activity. Instead of the psald gene, the alanine dehydrogenase gene from Bacillus sp. DSM730 was ligated downstream of the reg-psald gene. Alanine dehydrogenase activity was detected in a crude extract of E. coli JM109 cells harboring the resultant plasmid, pUpstrPAla by cultivation in DL-threo-β-phenylserine-containing LB medium. These results suggest that the reg-psald gene DL-threo-β-phenylserine dependency regulates the expression of the downstream psald gene. Therefore, we considered that the reg-psald gene was useful for construction of a gene expression system using DL-threo-β-phenylserine as an inducer.View full abstractDownload PDF (2125K) -
Akira Kurosawa, Shuhei Ikeda, Kana Sawada, Keiko Yamada, Morimichi Fuk ...Article type: Article
2010 Volume 84 Issue 2 Pages 47-52
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSThis study examined the relationship between Vitamin B_<12> (B_<12>) synthesized from Cobalt (Co) in the diet and actual excreted B_<12> in coprophagy-prevented or allowed rats fed either a commercial diet (B_<12>: 170μg/kg, Co: 1380μg/kg) or a purified diet (B_<12>: 25μg/kg, Co: 1.09μg/kg contain in B_<12>). Twelve healthy Sprague-Dawley strain male rats age 4 weeks were used in this experiment. Coprophagy was prevented for the first 4 weeks, then allowed for the following 7 days. We found that B_<12> excretion was greater than B_<12> intake in each diet group, and coprophagy-allowed rats excreted larger amounts of B_<12> than coprophagy-prevented ones. This suggests that for large Co intake in rats, synthesizable-B_<12> from Co-intake is excreted. However, for small Co-intake, the entire Co-intake was used by intestinal microorganisms in synthesizing B_<12>, but excreted even if it was insufficient.View full abstractDownload PDF (972K) -
Katsumi Shibata, Mika Endo, Maiko Yamauchi, Junko Hirose, Tsutomu Fuku ...Article type: Article
2010 Volume 84 Issue 2 Pages 53-58
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSNewly revised "Dietary Reference Intakes for Japanese (2010)", which is used from fiscal year 2010 to 2014, was published by the Ministry of Health, Labour and Welfare. This review is a summary of the papers which were used to decide the accepted values of the water-soluble vitamin contents of Japanese breast milk.View full abstractDownload PDF (661K) -
Katsuhito Kino, Teruhiko Kobayashi, Eiji Arima, Rie Komori, Takanobu K ...Article type: Article
2010 Volume 84 Issue 2 Pages 59-60
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
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Daisuke Nakata, Keiji TeraoArticle type: Article
2010 Volume 84 Issue 2 Pages 61-70
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
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Masaru SonodaArticle type: Article
2010 Volume 84 Issue 2 Pages 71-72
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (363K) -
Masaaki InabaArticle type: Article
2010 Volume 84 Issue 2 Pages 73-74
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (348K) -
Keiko Tazuya-Murayama, Motohiro Mishima, Kazuko YamadaArticle type: Article
2010 Volume 84 Issue 2 Pages 75-76
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
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Takashi TamuraArticle type: Article
2010 Volume 84 Issue 2 Pages 77-79
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 80-81
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (410K) -
[in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 81-
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (219K) -
[in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 82-83
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (422K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 83-
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (240K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2010 Volume 84 Issue 2 Pages 83-84
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 84-85
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (433K) -
[in Japanese], Ardiansyah, [in Japanese], [in Japanese], [in Japanese ...Article type: Article
2010 Volume 84 Issue 2 Pages 85-86
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (410K) -
[in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 86-87
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (409K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2010 Volume 84 Issue 2 Pages 87-
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (237K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 87-88
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (417K) -
[in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 88-89
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (412K) -
[in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 89-90
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (411K) -
[in Japanese], [in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 90-91
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (399K) -
[in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 91-
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (218K) -
[in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 91-92
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (413K) -
[in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 92-93
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (425K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
2010 Volume 84 Issue 2 Pages 93-94
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (297K) -
Article type: Bibliography
2010 Volume 84 Issue 2 Pages 95-98
Published: February 25, 2010
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (539K)
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