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Akio IWASHIMA, Hiroshi NISHIMURA, Hoyoku NISHlNO
Article type: Article
1984 Volume 58 Issue 12 Pages
577-587
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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The occurrence of thiamine-binding proteins in animal, plant and microbial sources has been known in recent years. We found two thiamine-binding proteins in Saccharomyces cerevisiae. A soluble thiamine-binding proteins present in the periplasm appeared to be a glycoprotein with a molecular weight of 140,000, while a membrane-bound thiamine-binding protein was found to be located in the cytoplasmic membrane of yeast cells. Some properties of these yeast thiamine-binding proteins are described. On the other hand, a thiamine-binding protein purified from rice bran was presumed to be composed of two identical subunits with a molecular weight of 50,000 which was not a glycoprotein. From the results obtained by chemical modification of these thiamine-binding proteins it was suggested that an ionic interaction between a carboxyl residue in the protein and a quaternary nitrogen atom of thiazole moiety of thiamine molecule plays a role in the binding of the thiamine-binding protein to thiamine.
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Atsuko TAKEUCHI, Toshio OKANO, Mariko SAYAMOTO, Setsuko SAWAMURA, Tada ...
Article type: Article
1984 Volume 58 Issue 12 Pages
589-595
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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(1) Distribution of vitamin D_2 and ergosterol in the parts of shiitake (Lentinus edodes), pileus, stipe and gills, were investigated by the determination of the two compounds by HPLC methods. The results showed that the contents of vitamin D_2 (_<IU>/100g) and erogosterol (μg/g) were highest in gills followed by pileus and stipe. (2) Both vitamin D_2 and ergosterol in Shiitake were confirmed to exist mainly as free forms by HPLC methods. The weight ratios of free forms of vitamin D_2 and ergosterol to the respective total amounts were 100% and 98.3%, respectively. (3) In despite of our results mentioned above, Ono et al. (Eiyo to Shokuryo, 26, 547-550, 1973) reported that the weight ratio of ester form of ergosterol to total content in Shiitake was 35-45% by using thin-layer chromatography and Liebermann-Burchard color reaction. Therefore, we performed a supplementary examination according to the method of Ono et al. The results showed that most of ergosterol existed as a free form (98.5% of weight ratio to the total), which agreed with the our results mentioned above.
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Emiko MORITA, Nobuyasu MIZUNO
Article type: Article
1984 Volume 58 Issue 12 Pages
597-601
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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To study B_6 absorption mechanism, we developed a method for simultaneous determination of pyridoxal 5'-phosphate (PLP), pyridoxal (PL), pyridoxine and 4-pyridoxic acid in blood and plasma using high-performance liquid chromatography. Our method employs a reverse-phase column (JASCO Fine SIL C_<18> T), an acidic potassium phosphate mobile phase (0.2% NaH_2PO_4-0.085% H_3PO_4 10:11 pH 2.45) and fluorometric detection (Ex 296nm, Em 391nm). This method is a rapid and simple, and requires smaller amounts of samples (50μl) than other methods. This method was employed to examine luminal absorption of PLP in situ. When PLP (5×10^<-3>M) dissolved in 80mM phosphate buffer was administered into the lumen of a rat ileal segment, 0.68% of PLP administered appeared as PLP and 2.88% as the sum of PLP+PL in the plasma of venous blood collected from the segment for 2 hr.
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
603-
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
603-604
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
604-
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
605-
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
605-606
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
606-607
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
607-
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
607-608
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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[in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
609-
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1984 Volume 58 Issue 12 Pages
609-610
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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[in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
611-
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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[in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
611-613
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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[in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
613-614
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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[in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
614-616
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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[in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
616-617
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
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[in Japanese]
Article type: Article
1984 Volume 58 Issue 12 Pages
617-618
Published: December 25, 1984
Released on J-STAGE: March 10, 2018
JOURNAL
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