VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 39, Issue 6

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[title in Japanese]

[in Japanese]

Article type: Article
1969 Volume 39 Issue 6 Pages 367-372
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_367

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A STUDY ON YELLOW COLORING OF THE PHARMACEUTICAL OINTMENTS CONTAINING VITAMIN A AND E

Kiyoshi MASANI, Goichiro KATSUI

Article type: Article
1969 Volume 39 Issue 6 Pages 373-376
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_373

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This paper presents the results of our investigation on the cause of yellow coloring of pharmaceutical ointments containing vitamin A, D and E. Yellowish substance of the colored ointments was analyzed by cdumn-, thin layer- and gas-chromatographies, UV-spectra, IR-spectra, and NMR. It was identified as 3,5,3', 5'-tetra-tert-butylstilben-4,4'-quinone. This compound is one of the oxidation products of BHT which is contained in vitamine A preparation as antioxidant. a-Tocopherol is a trigger of the oxidative reaction of BHT.

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LOCALIZATION OF VITAMIN D-DEPENDENT CALCIUM BINDING PROTEIN IN RAT INTESTINAL MUCOSA

Sachiko MORIUCHI, Kumiko OOIZUMI, Norimasa HOSOYA

Article type: Article
1969 Volume 39 Issue 6 Pages 377-381
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_377

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Rat intestinal mucosa was divided to the proximal and the distal parts, and the ^<45>Ca-binding of their supernatant fraction (38,000×g, 20min) was analyzed by Sephadex G-25 column equilibrated with ^<45>Ca, ^<1)> Three peaks (I, II, III) were observed in the elution profile of the 750mμ absorbancy accompanying through the column, and two ^<45>Ca peaks (IA, IB) were corresponding with peak I.^<2)> The protein corresponding to IA was observed in the distal part rather than the proximal and increased by vitamin D_3 administration. The protein corresponding to IB was almost equal amounts in both parts. The IB in distal part was not increased with D_3,and that in the proximal was increased. Vitamin D_3 would stimulate the biosynthesis of protein IA, and then stimulate the diffusional process of calcium absorption. Active transport will be stimulated with the secondary effect.^<3)>

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STUDIES ON RIBOFLAVIN-TETRANICOTINATE : (VIII)INCORPORATION OF THE NICOTINIC ACID MOIETY OF ORALLY ADMINISTERED RIBOFAVIN-TETRANICOTINATE INTO BLOOD STREAM AND TISSUE NAD

Akira KOTAKI, Keiichi KATO, Torao SAKURAI, Okiya TERADA, Kunio YAGI

Article type: Article
1969 Volume 39 Issue 6 Pages 382-385
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_382

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Rats were administered orally with B_2-Nic^<*1)>-^3H (only nicotinate moieties were uniformly labeled), and the incorporation of radioactivity into blood stream and into NAD of the kidney and the liver was investigated. A very slow increase in the specific radioactivity was observed with free NiA^<*2)>-^3H. After 30 hours of administration, however, the specific radioactivity of blood nicotinate of rats administered with B_2-Nic-^3H became higher than that of rats administered with NiA-^3H, suggesting the deposit type nature of the former compounds. The rate of incorporation of radioactivity of B_2-Nic-^3H into NAD of organs was also found to be slow as compared with the rate of incorporation of NiA-^3H. However, B_2-Nic-^3H was found to be superior to NiA-^3H in keeping the high level of specific radioactivity of hepatic and renal NAD for a long time.

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STUDIES ON O-NICOTINYLTHIAMINE DISULFIDE : (III)BIOLOGICAL PROPERTIES

Takaaki KIKUCHI, Masakazu TAKEMURA, Minoru KANAZAWA, Toshio KURODA

Article type: Article
1969 Volume 39 Issue 6 Pages 386-390
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_386

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Some biological properties of O-nicotinylthiamine disulfide (NTDS) was investigated. (1) Its thiamine activity was approximately equivalent to that of thiamine hydrochlorides in thiamine-deficient rats. (2) Oral administration of NTDS resulted in for higher thiamine level in the blood as well as on the liver and kindney. (3) After oral administration of NTDS to mouse resulted in long retention of thiamine concentration in the whole body. (4) One hour after administation of NTDS for intestinal wall brought about higher thiamine level than thiamine. (5) NTDS was definitely resistant to thiaminase I or II produced by Bacillus thiaminolyticus or B. aneurinolyticus, respectively.

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STUDIES ON THEIAMINE DISULFIDE : (XXXIV)ON THE TRANSPORT OF THIAMINE IN ERYTHROCYTES

Kiyoshi HARADA, Ichimonji SAITO, Isamu UTSUMI

Article type: Article
1969 Volume 39 Issue 6 Pages 391-395
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_391

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The transport of thiamine in erythrocytes was investigated and the rate constant of its transport was estimated. The transport rate of thimine was very slow, in contrast to its lipophilic derivatives or to the decreasing rate of thiamine level in blood following intravenous administration. It was confirmed by the following reasons that the transport process of thiamine had taken place by simple diffusion in its concentration above 3 μg/ml. (l) The kinetic equation induced from simple diffusion was able to be adapted to the transport pocess of thiamine in erythrocytes. (2) The transport rate constant was independent of thiamine concentrations. (3) The transport was not affected by the metabolic inhibitors, pyrithiamine, glucose and others. (4) Almost the same rate constant was observed between influx and efflux transport of thiamine in erythrocytes suspension. From the observation of the effect of temperaturre on the transport, the activation energy was estimated to be 35 Kcal/mole. Comparing with the transport rate in rat, rabbit, dog and human erythrocytes, it was recognized that the rate was higher in the above order.

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RESEARCH ON ASCORBIC AND ITS COMPLEX : (I)OXIDATIVE DECOMPOSITION OF ASCORBIC ACID

Akira HATANO

Article type: Article
1969 Volume 39 Issue 6 Pages 396-398
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_396

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Autoxidation of ascorbic acid starts with the dehydrogenation reaction of OH^- which binds 2C-3C and decomposition is accelerated by the activition of the di-radical. Kinetic analysis was carried out according to the theory of elecrochemical transfer coefficient of hydrogen ion. It was found that the oxidation of ascorbic acid is most active at pH 4.0. The decomposition mechanism of ascorbic acid is greatly inf1uenced by pH when oxidation or reduction is involved. The decomposition of ascorbic is assumed to occur in 2 ways, mamely, by either by H^+ of OH in 2C-3C being withdrawn as proton, as mentioned above, or H-attacking the π electrons of the oxygen in the lactone system.

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RESEARCH ON ASCORBIC ACID AND ITS COMPIEX : (II)BEHAVIOR OF MAGNESIUM IONS ON ASCORBIC ACID MONOSTEARATE-NICOTINAMIDE COMPLEX

Akira HATANO

Article type: Article
1969 Volume 39 Issue 6 Pages 399-401
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_399

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Mg^<2+> was added to ascorbic acid monostearate-nicotinamide complex and its behavior was investigated. When the concentration of ascorbic acid monostearate-nicotinamide is held constant and the concentration of the Mg^<2+>increased, chelate compound will be formed, one mole for each mole of Mg^<2+>. Formation constant of this chelate compound is 2. 09 and formation of chelate ring will depend greatly on its entropy. The dissociation constant obtained for this chelate compound is 2. 16×lO^<-10> and Mg^<2+> will produce unstable chelate compounds from ascorbic acid monostearate-nicotinamide complex.

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EXPERIMENTAL STUDIES ON THE INFLUENCE OF VITAMIN D UPON THE FEMALE SEXUAL FUNCTION

Naohiko KUDO

Article type: Article
1969 Volume 39 Issue 6 Pages 402-427
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_402

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To investigate the influences of vitamin D on female sexual function, Wistar strain rats were fed on vitamin D-free diet or were administered with vitamin D. (1) By feeding on vitamin D-free diet the sexual cycle of the rats were put out of order. In the adenohypophysis, α-cells decreased throughout the period of the experiment and δ-cells increased in just the early period. Atrophy of the adrenal cortex and the ovaries were seen after the middle stage of the period. Uterine atrophy began in early period. (2) In proportion of the dosis of vitamin D_2,both α- and β-cells were increased in the adenohypophysis, and functional acce leration of the adrenal cortex and ovaries were observed with uterine hypertrophy. Administration of vitamin D_2 was seemed to inhibit the castration changes of the adenohypophysis to some extent. (3) Vitamin D-deficiency caused marked interference to fertility and injury to fetal development owing to early atrophy of the syncytial trophoblast in the placental labyrinth in pregnant rats. After all, vitamin D deficiency brings on the sexual disorder to the female.

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INHIBITORY EFFECT OF PHENYLTHIAZINOTHIAMINE ON THE BIOSYNTHESIS OF THIAMINE BY SACCHAROMYCES CEREVISIAE

Toshihiro IIO

Article type: Article
1969 Volume 39 Issue 6 Pages 428-431
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_428

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Biosynthesis of thiamine Lrom 2-methyl-4-aminomethylpyrimidine (AMP) by Sacch. cerevisiae was greatly inhibited at the presence of phenylthiazinothiamine (PTT) but biosynthesis from 2-methyl-4-amino-5-hydroxymethylpyrimidine (OMP), instead of AMP, with PTT was shown to be nearly equal to that without PTT. Biosynthesis from OMP or AMP by crude enzyme from Sacch. cerevisiae, however, was proved to have no inhibition at the presence of PTT. AMP alone, when incubated with yeast cells, had been uptaken by cells from the medium more completely than OMP (this was proved by biosynthesis of thiamine with thiazole moiety by the washed cells after the incubation) . Addition of PTT to the medium was shown to give strong inhibition on uptake of AMP but practically no inhibition on that of OMP. It was suggested that the inhibitory effect of PTT on the biosynthesis of thiamine from AMP was due to the inhibition of incorporation of AMP to the yeast cells.

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Article type: Article
1969 Volume 39 Issue 6 Pages 432-
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_432_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 432-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_432_2

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Article type: Article
1969 Volume 39 Issue 6 Pages 432-433
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_432_3

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Article type: Article
1969 Volume 39 Issue 6 Pages 433-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_433_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 433-
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_433_2

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Article type: Article
1969 Volume 39 Issue 6 Pages 433-434
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_433_3

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Article type: Article
1969 Volume 39 Issue 6 Pages 434-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_434_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 434-435
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_434_2

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Article type: Article
1969 Volume 39 Issue 6 Pages 435-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_435_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 435-
Published: June 25, 1969
Released on J-STAGE: February 21, 2018

DOIhttps://doi.org/10.20632/vso.39.6_435_2

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Article type: Article
1969 Volume 39 Issue 6 Pages 436-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_436_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 436-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_436_2

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Article type: Article
1969 Volume 39 Issue 6 Pages 437-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_437_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 437-438
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_437_2

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1969 Volume 39 Issue 6 Pages 438-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_438_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 438-439
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_438_2

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1969 Volume 39 Issue 6 Pages 439-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_439_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 439-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_439_2

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1969 Volume 39 Issue 6 Pages 440-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_440_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 440-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_440_2

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Article type: Article
1969 Volume 39 Issue 6 Pages 440-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_440_3

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Article type: Article
1969 Volume 39 Issue 6 Pages 441-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_441_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 441-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_441_2

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Article type: Article
1969 Volume 39 Issue 6 Pages 441-442
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_441_3

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Article type: Article
1969 Volume 39 Issue 6 Pages 442-443
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_442

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Article type: Article
1969 Volume 39 Issue 6 Pages 443-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_443_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 443-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_443_2

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Article type: Article
1969 Volume 39 Issue 6 Pages 443-444
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_443_3

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1969 Volume 39 Issue 6 Pages 444-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_444_1

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Article type: Article
1969 Volume 39 Issue 6 Pages 444-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_444_2

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Article type: Article
1969 Volume 39 Issue 6 Pages 445-
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Article type: Article
1969 Volume 39 Issue 6 Pages 445-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_445_2

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Article type: Article
1969 Volume 39 Issue 6 Pages 445-446
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_445_3

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Article type: Article
1969 Volume 39 Issue 6 Pages 446-
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DOIhttps://doi.org/10.20632/vso.39.6_446_1

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1969 Volume 39 Issue 6 Pages 446-
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Article type: Article
1969 Volume 39 Issue 6 Pages 446-447
Published: June 25, 1969
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Article type: Article
1969 Volume 39 Issue 6 Pages 447-
Published: June 25, 1969
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[title in Japanese]

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Article type: Article
1969 Volume 39 Issue 6 Pages 447-
Published: June 25, 1969
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Article type: Article
1969 Volume 39 Issue 6 Pages 447-448
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_447_3

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Article type: Article
1969 Volume 39 Issue 6 Pages 448-
Published: June 25, 1969
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DOIhttps://doi.org/10.20632/vso.39.6_448_1

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