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Kenji FUKUZAWA, Hisako CHIDA, Setsuhiro OUCHI, Akira TOKUMURA, Hiroaki ...
Article type: Article
1982 Volume 56 Issue 12 Pages
641-652
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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We examined the antioxidant activities of various tocopherols (Toc) and their models under as nearly physiological conditions as possible, using egg lecithin liposomes as models of biomembranes. Toc can be exert the antioxidative effects without being oxidized when sufficient Toc to inhibit lipid peroxidation completely were incorporated in the liposome membranes, but when less quantities of Toc were incorporated, they were decomposed with increase in lipid peroxide formation. Cholesterol did not act as an antioxidant, even when incorporated in large amount into the liposome membranes, but it increased the antioxidative efficiency of α-Toc. The activities of Toc on ascorbic acid-Fe^<2+>-induced lipid peroxidation increased in order α-Toc>β-Toc>γ-Toc>δ-Toc>tocol, in agreement with their known order of biological vitamin E potencies. α-Toc model, in which the isoprenoid chain of α-Toc replaced by propionic acid, showed less activity. One molecule each of α-, β-, γ-, δ-Toc and tocol were calculated to be able to protect 220, 120, 100, 30 and 20 molecules, respectively, of polyunsaturated fatty acids. The ratio of α-Toc to polyunsaturated fatty acid moieties corresponds to that of membranes in highly oxygenated tissues such as heart and lung. The possible mechanism of the high efficient inhibitory effects of the Toc on ascorbic acid-Fe^<2+>-induced lipid peroxidation in liposome membranes is discussed.
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Chiharu SAKAGUCHI, Yoko OTAWARA, Norimasa HOSOYA, Sachiko MORIUCHI, Hi ...
Article type: Article
1982 Volume 56 Issue 12 Pages
653-658
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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Vitamin K dependent γ-carboxyglutamic acid (Gla)-containing proteins (BGP) were purified from vitamin D deficient and normal chick femur cortical bones and the effect of vitamin D on their properties were observed. Between vitamin D deficient and normal chick BGP, there was no significant difference in amino acid composition, Ca^<2+> binding activity and inhibitory effect on crystallization of hydroxyapatite in vitro. These results suggest that the properties of BGP are not influenced by vitamin D.
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Hiroshi TAGUCHI, Kunio KONDO, Yoshihide SHIMABAYASHI, Kazuo IWAI
Article type: Article
1982 Volume 56 Issue 12 Pages
659-664
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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Nicotinamide deamidase (EC 3.5.1.19) was partially purified from hog liver acetone powder and properties of this enzyme were investigated. Molecular weight of this enzyme was estimated as 180,000 daltons. The enzyme was stable at pH between 6 and 8. The optimum pH of the enzyme reaction was found at 6.8. The K_m value was 0.126 M. This enzyme reaction was inhibited by p-chloromercuribenzoate, Fe^<3+>, Hg^<2+>, Al^<3+> and Co^<2+>. NAD, NADH and N^1_methylnicotinamide were also inhibitory. This enzyme reaction was found to be irreversible. These properties were compared with those of enzymes found in various species, and a possible function of the enzyme in NAD biosynthesis and metabolism was discussed.
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Kichiko KOIKE, Masahiko KOIKE
Article type: Article
1982 Volume 56 Issue 12 Pages
665-673
Published: December 25, 1982
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Article type: Appendix
1982 Volume 56 Issue 12 Pages
675-679
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
681-
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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[in Japanese], [in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
681-682
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
682-
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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Article type: Appendix
1982 Volume 56 Issue 12 Pages
683-
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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[in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
683-684
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
685-
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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[in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
685-686
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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[in Japanese], [in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
686-687
Published: December 25, 1982
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[in Japanese], [in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
687-688
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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[in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
688-
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
688-689
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
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[in Japanese], [in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
689-690
Published: December 25, 1982
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[in Japanese], [in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
690-691
Published: December 25, 1982
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
691-
Published: December 25, 1982
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[in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
693-694
Published: December 25, 1982
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[in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
694-695
Published: December 25, 1982
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[in Japanese], [in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
695-697
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1982 Volume 56 Issue 12 Pages
697-698
Published: December 25, 1982
Released on J-STAGE: March 10, 2018
JOURNAL
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