-
Hisashi MASUDOMI
Article type: Article
1961 Volume 23 Issue 4 Pages
229-236
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
Tryptophan metabolism of tryptophan auxotrophs and vitamin B_6 auxotrophs derived either from E. coli W strain or 0-20 standard strain was investigated. Of these, a strain named #102 revealed its peculiar nutritional requirements ; it was found that this organism required any one of three metabolites, tryptophan, serine or vitamin B_6. Indole was found to be accumulated in the culture medium of #102. The quantity of accumulated indole was found to be greatest when the organism was grown in the presence of serine and was smallest when grown in the presence of serine plus pyridoxal. The washed cell of this strain was capable of converting anthranilic acid to indole, and indole and serine to tryptophan. The synthesized tryptophan was increased in the presence of pyridoxal. The organism was also found to synthesize vitamin B_6,the amount of which was found to be comparable to that synthesized by the parent organism. From these experimental data, the author concluded that tryptophan synthetase of this strain had been altered in such a manner as synthesized vitamin B_6 could not be utilized efficiently. The author could not find any activity of tryptophanase in indole requireing mutants. Tryptophanase activity of tryptophan requireing mutants was, however, not impaired.
View full abstract
-
Kengo OKUMURA
Article type: Article
1961 Volume 23 Issue 4 Pages
236-237
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
The oxime of pyridoxal was converted to pyridoxamine by electrolytic reduction in 83.5-98.5% yield. In my apparatus, 0.1 to 0.2 gram of the oxime dissolved in 1-2N hydrochloric acid was reduced at 1.5-2.0 volts with lead electrodes.
View full abstract
-
Kengo OKUMURA
Article type: Article
1961 Volume 23 Issue 4 Pages
238-243
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
Ion exchange resin column chromatography with Dowex 50×4 was adopted to the separation of thiamine and its derivatives. Citrate buffer (pH 3.4 and 6.5) and phosphate buffer (pH 6.8) were used as the eluate. By this method they could clearly be differentiated from each other on the chromatogram.
View full abstract
-
Kengo OKUMURA
Article type: Article
1961 Volume 23 Issue 4 Pages
244-246
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
Ion exchange resin column chromatography with Dowex 50×4 was adopted to the separation of vitamin B_6 group. Acetate buffer (pH 5.5) was used as the eluate. By this method they could clearly be differentiated from each other on the chromatogram.
View full abstract
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
246-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
Kazuo HOTTA, Osamu ANDO
Article type: Article
1961 Volume 23 Issue 4 Pages
247-250
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
In the preceding paper, the authors reported that flavoviolet (1-ribityl-2-oxo-3-hydroxy-6,7-dimethylquinoxaline) was found in the leaves, when the above ground part of farm grown pea plant was water-cultured in riboflavin solution for several days. In this paper, the authors tried the isolation and purification of this substance in plant tissues and determination of the substance. The purpose was almost achieved by treating column chromatography with Amberlite IRA-400,IR-120 and Florisil.
View full abstract
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
250-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
250-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
250-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
250-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
Yutaka TOMODA
Article type: Article
1961 Volume 23 Issue 4 Pages
251-256
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
The effect of α-tocopherol phosphate on blood coagulation was investigated systematically in vitro. Changes by the addition of α-tocopherol phosphate of recalcified plasma clotting time, thromboplastin generation test, prothrombin time, thrombin time and fibrinolysis were observed. Recalcified plasma clotting time, prothrombin time and thrombin time were prolonged with increase of α-tocopherol phosphate concentration. Generation time of thromboplastin was delayed and its production decreased. Lysis time of fibrin clot was reduced. These results indicate that α-tocopherol phosphate acts as anticoagulant in the first and the third phase of blood coagulation, while activating fibrinolysis
View full abstract
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
256-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
256-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
Kunio YAGI, Jun OKUDA
Article type: Article
1961 Volume 23 Issue 4 Pages
257-261
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
Riboflavin-2', 3', 4', 5'-tetrapalmitate and riboflavin-2', 3', 4', 5'-tetrabutyrate were tested by dietary survey using male albino rats. The latter was found to promote the growth of the test animals and to prevent ariboflavinosis. The growth potency of the compound was found to be in nearly the same level as free ribofiavin. However, the former was found to have very low growth activity.
View full abstract
-
Chikataro KAWASAKI, Isao TOMITA
Article type: Article
1961 Volume 23 Issue 4 Pages
262-266
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
The daily dosis of 1mg of thiamine sulfuric acid ester (I) given intraperitonealy to albino rats on thiamine-deficient diet showed loss of their weights and even 10mg of (I) failed to promote their growth, while daily 10μg of thiamine-HCl in addition to 10mg of (I) showed the same growth curve as that responsible to 10μg of thiamine-HCl alone. The LD_<50> of (I) to mice was about 1mg/g weight and there was no difference between normal and thiamine-deficient groups. Thus no anti-thiamine activity of (I) to animals has been proved. One mg of (I) to 1μg of thiamine diphosphate showed no competitive inhibition to CO_2-evolution from pyruvate by yeast etiozymase, although (I) was proved to have a slight "Ochoa-effect"activity to etiozymase.
View full abstract
-
Kihachiro UEHARA, Masami MAKITA
Article type: Article
1961 Volume 23 Issue 4 Pages
266-269
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
The purification of vitamin K_3 reductase from sweet potato was attempted. The enzyme preparation which was about 5000 times more active per mg protein of crude extract and was almost homogeneous in ultracentrifugal analysis was obtained.
View full abstract
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
269-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
Kihachiro UEHARA, Masami MAKITA
Article type: Article
1961 Volume 23 Issue 4 Pages
270-275
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
Studies have been made on the properties of highly purified vitamin K_3 reductase from sweet potato. The solution of the enzyme shows a characteristic flavoprotein spectrum with maxima at 275,375 and 445mμ. The molecular weight calculated from its flavin content is about 40,000 and this value is in satisfactory agreement with that from sedimentation constant (2.86S). Evidence obtained from paper chromatography and reactivation experiment indicates that the prosthetic group is FMN. Both TPNH and DPNH serve as electron donator. Naphthoquinone derivatives, p-benzoquinone, 2,6-dichlorophenol-indophenol and ferricyanide as well as vitamin K_3 serve as electron acceptor but methylene blue, brilliant cresyl blue, cytochrome c, molecular oxygen etc. do not. Data have been presented to describe pH optima, stability, inhibitors and photooxidation of the enzyme. These results indicate that the enzyme is a hitherto unknown flavoprotein.
View full abstract
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
276-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
276-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
277-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
277-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
278-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
278-279
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
279-280
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
280-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
280-281
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
281-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
281-282
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
282-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
282-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
282-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
283-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
283-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
283-284
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
284-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
284-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
284-285
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
285-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
285-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
285-286
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
286-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
286-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
286-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
287-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
287-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
287-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
287-288
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1961 Volume 23 Issue 4 Pages
288-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1961 Volume 23 Issue 4 Pages
288-
Published: July 25, 1961
Released on J-STAGE: December 22, 2017
JOURNAL
FREE ACCESS