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Volume 24 (1961)
- Issue 4 Pages 223-
- Issue 3 Pages 125-
- Issue 2 Pages 55-
- Issue 1 Pages 1-
Volume 24, Issue 2
Displaying 1-26 of 26 articles from this issue
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Yoshitsugu NOSEArticle type: Article
1961 Volume 24 Issue 2 Pages 55-70
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
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Yasuto WATANABEArticle type: Article
1961 Volume 24 Issue 2 Pages 71-72
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSThiamine hydrochloride, thiamine dicetylsulfate, dibenzoylthiamine or thiamine monorhodanate were added to wheat flour and their stabilities in baking process were examined. Thiamine dicetylsulfate, dibenzoylthiamine and thiamine monorhodanate remained in the bread about 70 per cent of that added to the flour, while thiamine hydrochloride remained about 60 per cent.View full abstractDownload PDF (290K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 72-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
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Soichi NIWA, Nobu KATAYAMA, Syoko UNO, Kikue KANEMOTO, Ririko KOBAYASH ...Article type: Article
1961 Volume 24 Issue 2 Pages 73-79
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSStabilization effects of polyphosphoric acid for the decomposition of enriched vitamin C in the soft drinks containing food color, and its inhibiting effects for the decolorization of food color by the vitamin C were studied at pH 2.5-6.0. In the presence of polyphosphoric acid (0.1%, final concentration), the decomposition of vitamin C and decolorization of food color in the soft drinks were not observed at pH 2.5-3.0,even by heating at 90℃ for 30 min., storing at 20℃ for 10 days, or exposure to sun light for 2 days.View full abstractDownload PDF (713K) -
Takatomo HORIOArticle type: Article
1961 Volume 24 Issue 2 Pages 79-84
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESS1 mg% solutions of thiamine mixed with a sufficient amount of buffers between pH 6 and 13,were converted into thiochrome with varied yields by the addition of ferricyanide immediately after mixing. There was found two maximal points for yielding thiochrome, e.g. at pH 9〜10 and over pH 13. Thiamine was left partly unchanged at pH less than 9 and it was chiefly transformed into thiamine disulfide at pH between 11 and 12. If the buffered solution of thiamine was kept standing for 5 minutes at pH 11.5,its oxidation into thiamine disulfide by addition of ferricyanide was nearly complete and no thiochrome was formed, but formation of thiochrome was still observed at its optimal pH at 9 or over 13 even after standing for 30 minutes, although the quicker decrease of thiochrome formation happened over pH 13. As formation of thiamine disulfide was always demonstrated even at the optimal pH for thiochrome-formation, it was concluded that ferricyanide-oxidation of thiamine resulted in thiochrome and thiamine disulfide, to variable proportions, whereas the former was maximal over pH 13 and the latter maximal at pH 11.5.View full abstractDownload PDF (718K) -
Takatomo HORIOArticle type: Article
1961 Volume 24 Issue 2 Pages 84-87
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSOne mg % thiamine in buffered solutions over pH 11 showed absorption maximum at 340 mμ which faded out quickly, and the intensity of the absorption was highest at pH 13 and slowly fading. Formation of thiochrome by ferricyanide in these ranges of pH was also proportional to the intensity of absorption at 340mμ. Thiamine in NaOC_2H_5 or NaOCH_3 also showed the absorption at 340mμ which was lasting for many hours, while its formation of thiochrome by ferricyanide was rather diminishing on standing. If water was added into thiamine solutions in NaOCH_3,absorption at 340mμ and formation of thiochrome were quickly decreased. The absorption maximum at 340mμ would indicate an cyclic thiol form (I) of thiamine but its absorption was not always correlated to formation of thiochrome.View full abstractDownload PDF (505K) -
Takatomo HORIOArticle type: Article
1961 Volume 24 Issue 2 Pages 88-92
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSBy an alkaline ferricyanide solution, thiamine was oxidized into thiochrome and thiamine disulfide in different proportions which were variable according to pH of the solution. Moreover, dissolved oxygen in the solution gave a considerable influence to stimulate formation of thiamine disulfide and inhibit oxidation to thiochrome. But at pH between 11-12 formation of thiamine disulfide was predominant even at the exclusion of oxygen by saturating N_2 in the solution. At pH over 13,formation of thiochrome from 10μg thiamine was 83% to the theoretical value in the N_2-saturated solution and 58% in the O_2-saturated solution. If the O_2-saturated solution of thiamine was replaced by N_2,the yield of thiochrome was recovered as high as that of the initially N_2-saturated solution. Dissolved oxygen or its exclusion by N_2-saturation gave no influence to the absorption at 340mμ of an alkaline thiamine solution. So an assumed intermediate showing absorption at 340mμ would not be a key substance to formation of thiochrome, however dissolved oxygen will push the reaction to form thiamine disulfide and exclusion of oxygen makes it favorable to form thiochrome.View full abstractDownload PDF (586K) -
Yoshihiro TOKUDAArticle type: Article
1961 Volume 24 Issue 2 Pages 93-96
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSThe biological utilization of some thiamine derivatives was investigated by estimating the formation rate of cocarboxylase from the derivatives by rat liver supernatant. The time course of cocarboxylase formation was determined manometrically by using apocarboxylase extracted from bakers' yeast. Thiamine was most predominant, thiamine propyldisulfide and S-benzoylthiamine monophosphate was a little delayed in the cocarboxylase formation while thiamine-thioctate disulfide was almost same as thiamine and the formation rate from O-benzoylthiamine disulfide was smaller than the other thiamine derivatives.View full abstractDownload PDF (457K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 96-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (145K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 96-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (145K) -
Yoshitsugu NOSE, Shinichiro KAWASHIMA, Junitsu SAITOArticle type: Article
1961 Volume 24 Issue 2 Pages 97-101
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSThe amounts of α-lipoic acid and thiamine in whole blood, liver and kidney of albino rats were estimated after the oral administration of TATD-HCl (1.7mg/kg) and of thiamine hydrochloride (1.0mg/kg) and DL-α-lipoic acid (0.61mg/kg), and also determined the excreted amounts in 24 hours urine. The lipoic acid and thiamine concentration in liver and kidney was increased at 3 hours after the administration TATD. The both levels of lipoic acid and thiamine were greater than that of the animals administered thiamine and lipoic acid. The levels of thiamine in whole blood of the TATD group gave a higher value than that of lipoic acid and thiamine groups, while the levels of lipoic acid did not show remarkable difference between both groups. Urinary excretion of lipoic acid and thiamine was higher in the TATD group than the other. These results suggest that the intestinal absorption of TATD is superior to that of lipoic acid or thiamine.View full abstractDownload PDF (627K) -
Teijiro KAMIHARAArticle type: Article
1961 Volume 24 Issue 2 Pages 101-104
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSThe presence of an unknown substance in the mycelium of Aspergillus oryzae which was previously reported to stimulate the growth of Streptococcus faecalis 10C1 in the lipoic acid assay medium was confirmed by a method of bioautography using water-saturated n-butanol as the developing solvent and Stc. faecalis 10C1 as the test organism. The substance showed Rf value of 0.75-0.8,while α-lipoic acid and β-lipoic acid were detected at Rf 0.92 and 0.65,respectively. The behaviour of the substance on several ion exchange resins was compared with that of lipoic acid. Consequently, a complete separation of the substance from lipoic acid was successfully carried out by passing a hydrolysate of the mycelium through a column of Amberlite IR-4B.View full abstractDownload PDF (498K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 104-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (180K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 104-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (180K) -
Saburo FUKUI, Nobuko OISHIArticle type: Article
1961 Volume 24 Issue 2 Pages 105-112
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSThe action of oxythiamine on yeast changed from inhibitory to stimulatory at a certain stage of incubation. In this case disappearance of oxythiamine and increase of thiamine were proved by paper ionophoresis and chemical assay. Simultaneous addition of the pyrimidine moiety of thiamine with oxythiamine caused a considerable decrease of the decomposition of the antagonist and an increase of the vitamin synthesis. Oxythiamine, also, exhibited almost the same effect on E. coli thiazole-less mutant as thiamine and its thiazole-moiety, while the antivitamin had no effect on pyrimidine-less mutant. In the incubation products of oxythiamine with washed cells of yeast or E. coli, the occurence of the pyrimidine and thiazole moieties of oxythiamine was demonstrated by paper chromatography and paper ionophoresis. From these results it is considered that oxythiamine is cleaved to its two moieties and the thiazole part is utilized as a precursor of thiamine. However, oxythiamine and its pyrimidine moiety, respectively, exerted a growth effect on E. coli thiamine-less and pyrimidine-less mutants after 15 hours'lag period. In these mutants transformation of 4-hydroxyl group of pyrimidine to amino group is supposed.View full abstractDownload PDF (851K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 112-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
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Kunio OKUDAArticle type: Article
1961 Volume 24 Issue 2 Pages 113-120
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSThe principles and techniques for the determination of vitamin B_<12> binding power in biological materials are described for the benefit of the reader. This paper which is the first half of the article details the growth inhibition method and the dialysis method. The former consists of the determination of "free"vitamin B_<12> by direct addition of the material to the culture media and calculation of the "bound" from from the total and free vitamin B_<12> activities as measured microbiologically. The factors influencing the result are commented critically. The latter is subdivided into three techniques of "exhaustive", "equilibrium"and "partial"dialyses, and the advantages of the use of radioactive vitamin B_<12>, the source of error, experimental design and calculation are discussed for each technique.View full abstractDownload PDF (1148K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 120-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (142K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 120-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (142K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 120-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (142K) -
Article type: Appendix
1961 Volume 24 Issue 2 Pages 121-123
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (203K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 124-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (142K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 124-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (142K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 124-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (142K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 124-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (142K) -
[in Japanese]Article type: Article
1961 Volume 24 Issue 2 Pages 124-
Published: October 25, 1961
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (142K)
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