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Keiichi SAKAI, Yasuo SUZUKI, Kenji FUKUZAWA, Mitsuru UCHIYAMA
Article type: Article
1968 Volume 38 Issue 6 Pages
423-426
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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The rate of peroxide formation in the liver homogenate during the incubation was found to decrease by the addition of vitamin K_1. This effect was not observed under the variety of peroxide forming conditions in the absence of tissue. Vitamin K_1 was reduced by the NADH-K_1 reductase which was present in the soluble fractionn of liver and the reduced form of K_1 acted as a free radical traping substance. Dicoumarol, the specific inhibitor of the reductase, inhibited the protective effect of K_1 on the lipid peroxidation. These evidences suggest that vitamin K acts as an antioxidant under the physiological condition.
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Toshio ANMO, Mitsunori WASHITAKE, Yasuji TAKASHIMA, Seiichi SATO
Article type: Article
1968 Volume 38 Issue 6 Pages
427-433
Published: December 25, 1968
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In the previous paper, it was investigated the influence of water content on the change of vitamin A in aqueous ethanolic solution. In this paper, the authors report the similar studies on the stability of anhydrovitamin A, an intermediate product of vitamin A acetate, in aqueous ethanolic solution. Anhydrovitamin A was determined by spectrometric method and Carr・Price reaction. The amount of changed products of anhydrovitamin A were examined by alumina column chromatography and thin layer chromatography. The stability of anhydrovitamin A in 45-100% ethanolic solution decreased gradually as water increased. The values of remaining anhydrovitamin A by both methods were almost equal, therfore it is supposed that the Carr・Price reaction-positive intermediate is not formed. Two substances were recognized as changed products of anhydrovitamin A, the one, having maximum absorbances at 325 and 340mμ, is eluted prior to anhydrovitamin A, and the other, having maximum absorbance at 283mμ, is eluted last in column chromatogram. Retrovitamin A and unknown substances I, II which are reported previously as the changed products of vitamin A acetate were not recognized. The reversible reaction of anhydrovitmin A to vitamin A alcohol or its acetate was not observed.
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Shoichi MASUSHIGE, Takao SUZUKI, Yoshikazu SAHASHI
Article type: Article
1968 Volume 38 Issue 6 Pages
434-436
Published: December 25, 1968
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In previous paper, it was shown that the oral supplementation of 75μg per day of riboflavin on rat cause an increase of liver xanthine oxidase and an accumulation of riboflavin, FAD and FMN in liver. In this paper, the further experiment with riboflavin-deficient rat was done to elucidate the mechanism of the increase of xanthine oxidase activity. A marked increase of liver xanthine oxidase activity was observed when 1mg of inosine and 15 or 3Oμg of riboflavin are supplemented, whereas a significant relation between enzyme activity and accumulation of riboflavin in liver could not demonstrated. Therefore, the authors intend to conclude that the increase of xanthine oxidase activity is due to an induced reaction of inosine.
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Etsuji TAKASAKI
Article type: Article
1968 Volume 38 Issue 6 Pages
437-440
Published: December 25, 1968
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The influence of thiamine deficiency on urinary excretion of oxalic acid in albino rats was studied. The oxalic acid in urine was estimated by Zarembski・Hodgkinson's method. The increasing excretion of oxalic acid in thiamine deficient rats was found and it suggests that the deficiency of thiamine blocks the pathway of glyoxylic acid to formic acid+CO_2 and increases the urinary excretion of oxalic acid.
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Junnosuke YAMAMOTO, Ikuo HIRAKO, Hiroshi OYA, Keiko YAMADA, Kiyoshi MA ...
Article type: Article
1968 Volume 38 Issue 6 Pages
441-446
Published: December 25, 1968
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Vascular actions of riboflavin-2', 3', 4', 5'-tetranicotinate (B_2-Nic) were investigated with the use of various parameters : the outflow from the isolated rabbit ear, the diameter of the intact dog ear vessels, blood pressure of the rabbit and the contractile force and heart rate of the isolated guinea pig atria. B_2-Nic produced a vasodilation in the isolated rabbit ear and intact dog ear, and caused a hypotensive response in the rabbit. This depressor response was probably produced by the peripheral vasodilation in the rabbit, because B_2-Nic had no effect on the contractile force and heart rate in the isolated atria of guinea pig.
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Kiku MURATA, Eiko MAEKAWA, Keiko IWASHITA
Article type: Article
1968 Volume 38 Issue 6 Pages
447-452
Published: December 25, 1968
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Thiamine value of blood and amount of thiamine excretion from urine after the oral administration of 1-400mg (equivalent to thiamine hydrochloride) of dicarbethoxythiamine (DCET) in the form of one or two pills just after a breakfast to healtry young women were investigated comparing with those of thiamine and thiamine disulfide. Highest value of thiamine in blood was around 3 hours after DCET administration and decreased to a normal level after 24hours, but remained higher than a normal level when more than 200mg of DCET were given. Thiamine value of blood 3 hours after the administration more than 5mg of DCET was higher than those given equivalent amounts of thiamine or thiamine disulfide. The increase of thiamine excretion from urine during 4 and 24 hours after administration of DCET and the intake of DCET were almost linear, which was not observed when thiamine or thiamine disulfide were administered.
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Kiyoshi HARADA, Ryoichi HIGUCHI, Isamu UTSUMI
Article type: Article
1968 Volume 38 Issue 6 Pages
453-458
Published: December 25, 1968
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The respiration of baker's yeast was found to be stimulated by the addition of thiamine and inhibited with sorbate. However, during the studies on the sorbate inhibition concerned with the yeast respiration using pyruvate as substrate, the authors found that the coexistence of thiamine and sorbate has a significant inhibitory effect on the respiration. Butyrylthiamine disulfide or thiamine diphosphate in place of thiamine show the same effect, but none of benzoate, salicylate, dehydroacetate or propionate in place of sorbate show the effect. This characteristic effect was also observed with the substrate, glucose or acetate. The inhibitory effect was also found at the growth of the microorganism under the condition of shaking culture, but, at stationary condition this effect was no longer observed. The fact that this effect is hardly observed in the cells containing high level of CoA would give us an available suggestion for the inhibition mechanism.
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Chikataro KAWASAKI, Takeo KISHI, Masahiro NISHII
Article type: Article
1968 Volume 38 Issue 6 Pages
459-463
Published: December 25, 1968
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Modified thiamine compounds were easily converted to thiamine in the culture of E.coli. Disulfide-compounds, such as thiamine propyldisulfide (TPD) and O-benzoylthiamine disulfide were more easily converted to thiamine than S-acyl-compounds, such as O, S-dibenzoylthiamine and O, S-dicarbethoxythiamine. TPD was reduced to thiamine by the cell-free extract (CFE) of E.coli but not by the cell debris. TPD was more strongly reduced to thiamine by the CFE in its alkaline range than in its neutral range and at higher temperature (60〜100℃) than 37℃. The CFE, when heated for 5 min., was shown to reduce TPD more strongly but when heated for over 30 min., it was partly inactivated to reduce TPD. Such SH-blocking reagents as PCMB, NEM and MIA, when added to the CFE, or dialysis of the CFE weakened the reduction of TPD by the CFE. Thus SH-groups of protein in the CFE were shown to be responsible to the reduction of TPD by the CFE.
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Masashi MATSUNAGA
Article type: Article
1968 Volume 38 Issue 6 Pages
464-466
Published: December 25, 1968
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The oxalic acid in the urine was measured to know whether the oxalic acid in the urine is increased after an oral administration of a large amount of ascorbic acid. The enzymatic determination method by Mayer・Ribeiro was used. Two g of ascorbic acid was given daily to five patients in absolute rest and the measurement was performed on the 6,7,13,14,20,21,27 and 28th day. The blind test of the oxalic acid in the urine was done for the last two days before the experiment. In 2 cases 5 g of sodium bicarbonate was given together with 2 g of ascorbic acid. It was noted that the urinary excretion of the oxalic acid had a tendency of increasing by the administration of ascorbic acid in some cases.
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[in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
467-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
467-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
467-468
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
468-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
468-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
468-469
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
470-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
471-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
471-472
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
472-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
472-473
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
473-474
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
474-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
474-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
474-475
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
475-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
475-476
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
476-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
476-477
Published: December 25, 1968
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[in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
477-
Published: December 25, 1968
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[in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
477-478
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1968 Volume 38 Issue 6 Pages
478-
Published: December 25, 1968
Released on J-STAGE: February 17, 2018
JOURNAL
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