-
Kenji KUMAKI, Masafumi FUKUSHIMA, Toshiko SHIMIZU, Machiko OGAWA, Yosh ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
399-408
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
When injected i. v., ^3H-25-OH-D_3 was significantly excreted into the urine in the experimental nephritic rat of Shibata et al. (30〜40% radioactivity), while in control rat this was 1〜2%. Excretion of ^3H-1α-OH-D_3 was not much in the nephritic rat (1〜10%) than in the control rat (1〜2%). There were good linear relationship between the amount of protein and total radioactivity in 24 hour urine of the nephritic rat injected i. v. ^3H-1α-OH-D_3 or ^3H-25-OH-D_3. In the nephritic rat, metabolism of 25-OH-D_3 to 24,25-(OH)_2-D_3 was significantly injured while metabolism of 1α-OH-D_3 to 1α, 25-(OH)_2-D_3 was almost the same as that of the control rat. It can be assumed that the enhanced excretion of ^3H-25-OH-D_3 in the nephritic rat was due to the increase in excretion of 25-OH-D_3 binding plasma protein, α-globulin, while little lipoprotein which may bind 1α-OH-D_3 was excreted.
View full abstract
-
Keiko FURUTA, Atsuko ADACHI, Tadashi KOBAYASHI
Article type: Article
1978 Volume 52 Issue 9-10 Pages
409-415
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
A routine method for the determination of vitamin D in commercial fortified dried milks was established by using high-performance liquid chromatography (HPLC). The method includes extraction of the fat, alkalisaponification, isolation of the unsaponifiable matters, digitonin-Celite and Florex-XXS column chromatography, thin-layer chromatography (TLC) and HPLC. Since co-chromatography performed by mixing the sample solution with authentic vitamin D_2 gave mono peak, vitamin D in the fortified samples was confirmed to be vitamin D_2. Although the samples contained comparatively large amounts of sterols, vitamin A, E, their decomposed products and other interfering materials, their influences could be eliminated by the procedure. When the proposed method was applied to 5 kinds of commercial samples, the assayed values of vitamin D_2 were between the range of 98.0%〜126.3% to each indicated value. From these results, the proposed method were confirmed to be useful for the determination of vitamin D in commercial fortified dried milks.
View full abstract
-
Yoshikazu HINOHARA, Yasuo TOHIRA, Hiroshi KAMIYAMA, Machiko OGAWA, Hid ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
417-423
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
The absorption, distribution, excretion and metabolism of 2-^3H-1α-OH-D_3 (4.2Ci/mmol, 4.3μg/40.3μCi/head) in rhesus monkey were studied after administered in gastrointestine pernasally. 1) Peak blood levels (0.004% of administered radioactivity/ml, respectively) were observed at both 9 and 18〜24 hours with apparent half life of 24 hours. 2) There was no specific distribution of radioactivity in the tissues studied. 3) Until 48 hours, 2.5 and 45% of the administered radioactivity were recovered in the urine and feces respectively. 4) Both ^3H-1α-OH-D_3 and ^3H-1α, 25-(OH)_2-D_3 were detected in all the tissues examined. The latter was predominant than the former in the blood and liver. 5) In the bile collected at 9 hours, ^3H-1α-OH-D_3 and ^3H-1α, 25-(OH)_2-D_3 were detected in a few percent of the total radioactivity recovered. 6) The above results were compared with these of rats obtained in the earlier study.
View full abstract
-
Kasturi Sengupta, AjayK. Chatterjee
Article type: Article
1978 Volume 52 Issue 9-10 Pages
425-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
425-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
425-426
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
426-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
426-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
426-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
426-427
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
427-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
427-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
Praneet Pongpaew, Sastri Saovakontha, FrankPeter Schelp, Venus Supawan ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
427-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
427-428
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
428-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
428-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
428-429
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
429-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
430-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], RobertS.H. ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
431-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
431-432
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
432-433
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
433-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
433-434
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
435-436
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1978 Volume 52 Issue 9-10 Pages
436-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
437-
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
437-438
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
439-440
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
441-442
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
442-443
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1978 Volume 52 Issue 9-10 Pages
443-446
Published: October 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL
FREE ACCESS