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Yoshito NISHIZAWA
Article type: Article
1967 Volume 35 Issue 6 Pages
421-435
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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Satoshi IDE
Article type: Article
1967 Volume 35 Issue 6 Pages
436-443
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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Lipoamide dehydrogenase has been isolated from acid-precipitated human liver particles in a highly purified state by a freezing and thawing technique instead of the heat treatment used by other workers. The enzyme was found to have a molecular weight of 138,000,and to contain 2 moles of FAD per mole of protein. In these respects, as in substrate specificity, Michaelis constant, absorption spectrum, and kinetic parameters, the enzyme is similar to preparations isolated from beef liver, pig heart and bacteria. This enzyme catalyzed the reduction of NAD by dihydrolipoamide and exhibited diaphorase and transhydrogenase activities. If the enzyme was incubated with the dithiol inhibitors such as arsenite and cadmium chloride in the presence of NADH_2,lipoamide dehydrogenase and transhydrogenase activities were strongly inhibited, but diaphorase activity was enhanced.
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Toshio NIIMURA, Shigeru SHIMADA
Article type: Article
1967 Volume 35 Issue 6 Pages
444-450
Published: June 25, 1967
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The formation of biotin from DL-desthiobiotin by washed cells and protoplasts of Bacillus megaterium was investigated. When the cells were fractionated into cell wall, cytoplasmic membrane and cytoplasm after the formation of biotin, the greater part of the synthesized biotin was found to be present in the cytoplasm. It was also found that the protoplasts could synthesize biotin as well as the intact cells. However, the amount of synthesized biotin was only about 1/2 of that to be expected in the washed cells. The biotin-synthetic activity of the treated protoplasts was lost as the structure was disintegrated (by phase contrast microscopy). That is, the protoplasts keeps clearly the form and the cytoplasm maintains the ability of the formation, but when the protoplasts are bursted by osmotic shock the ability is lost completely.
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Masako YAMADA, Noriko INAKOSHI, Tohru TSUKAHARA
Article type: Article
1967 Volume 35 Issue 6 Pages
451-456
Published: June 25, 1967
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In the preceding report of this series, a cup-plate method with Streptococcus faecalis as test organism was found to be available for the microbiological assay of pure folic acid solution from the basis of its high precision and the presence of a linear relation between the sizes of growth zones and the logarithms of the vitamin concentration. In order to confirm the availability of this method for the determination of folic acid in biological materials, several animal and vegetable samples were used and parallel assay was made with the turbidimetric method. In the case of the assay of free folic acid of some materials, the response lines from the extracts were not parallel to that from pure folic acid solutions. Also, the recoveries of the added folic acid to these extracts were unsatisfactory. This phenomenon is presumably due to the presence in the samples of growth factors or some factors affecting the assay results. The valid and reliable assays of folic acid in natural products by the plate method are not possible unless an improvement in extracting procedure of the vitamin from them will be detected.
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Masako YAMADA, Noriko INAKOSHI, Tohru TSUKAHARA
Article type: Article
1967 Volume 35 Issue 6 Pages
457-461
Published: June 25, 1967
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The application of a thin agar plate technique to the cup-plate method for the assay of folic acid resulted in a marked increase in the sensitivity, although it incured a lowering of the reproducibility, that is, the precision of the method. Thus, the cup-plate method using a thin ager plate was capable of determining of folic acid in biological materials containing a small quantity of the vitamin and it was effective for improvement of the recovery rates of the added vitamin to these materials. Some of them, however, were unsatisfactory in the recoveries and the dose-response lines for the samples were not parallel from that for standard folic acid solution. In order to eliminate such disadventages encountered in the plate method, the test solutions from natural products, free from the factors affecting the assay results must be obtained.
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Hidefumi WATANABE, Yasuo OSHIMA
Article type: Article
1967 Volume 35 Issue 6 Pages
462-469
Published: June 25, 1967
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Mice at the growing stage were subjected to forced exercises, which continued for many hours at a time and lasted a long period of time. They were examined for the state of growing during and after the period of forced exercises, so that the effect of these exercises might be clarified. In addition, studies were made to elucidate the effect of the vitamin B-complex (thiamine, vitamin B_6,pantothenic acid, pantethine) upon the process of growth of mice suffering from fatigue. As a result, recovery to growth, as well as growth itself, was inhibited in the mice both during and after the period of forced exercises. Administration of pantethine displayed a remarkable effect to improve the inhibited growth, while those of thiamine, vitamin B_6 or pantothenic acid were not so effective. Besides, forced exercises were imposed upon mice which were allowed to take only a limited amount of drinking water. After the period of exercises there was an increase in the amount of intake of water. When this increase, which had been brought about by the compensatory function, was induced to a noticeable extent, it was a little effective for the recovery of growth in the mice.
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Hiroyasu OTSUKA
Article type: Article
1967 Volume 35 Issue 6 Pages
470-473
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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The concentrations of ^<35>S in the cerebsospinal fluid after intravenous injection of thiamine tetrahydrofurfuryldisulfide (TTFD)-^<35>S(inner) and thiamine propyldisulfide (TPD)-^<35>S (outer) or thiamine-^<35>S in dog were determined. Though ^<35>S in the cerebrospinal fluid in the case of TTFD-^<35>S (inner) or thiamine-^<35>S increased very slightly, ^<35>S in the case of TPD-^<35>S (outer) increased markedly. From the results of this study, it seems that the penetration of thiamine from blood to cerebrospinal fluid is difficult.
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Minoru HAMADA, Taro HAYAKAWA, Tetsuji YAMAGUCHI, Masahiko KOIKE
Article type: Article
1967 Volume 35 Issue 6 Pages
474-484
Published: June 25, 1967
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When thiamine tetrahydrofurfuryldisulfide (TTFD) and other thiamine disulfide derivatives were incubated with human erythrocyte, a detectable amount of free thiamine has been noted in the incubation mixture in addition to intracellular accumulation of thiamine. Results showed that hemoglobin has the major role of the reduction of thiamine disulfide derivatives to form free thiamine in erythrocytes. It was also confirmed that this free thiamine was converted in vitro to thiamine diphosphate in the presence of adenosine triphosphate by thiamine pyrophosphotransferase from pig heart. When native or denatured purified human hemoglobin was incubated with TTFD, the former required two times amount of the latter to form a certain amount of free thiamine at 37℃. The contents of active thiol were calculated to be 1.68 moles per mole of native hemoglobin and 3.2 moles per mole of denatured one by the titration with PCMB. Sephadex gel filtration studies of the incubation mixture with ^<35>S-labeled TTFD (outer) and hemoglobin or glutathione indicated the formation of several radioactive compounds. It was also noticed that the disulfide compound produced from TTFD with hemoglobin was more labile than that of glutathione. From these results it was presented that the possible reaction mechanism between thiol groups of hemoglobin and thiamine disulfide derivatives was as follows : α_2(-SH)_2 β_2(-SH)_4+2B_1-S-S-R ⇄ α_2(-SH)_2 β(-SH)_2(-S-S-R)_2+2B_1
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Shoichi YAMAGATA, Yoshio GOTO, Jin KIKUCHI, Yuichi YAMAUCHI
Article type: Article
1967 Volume 35 Issue 6 Pages
485-493
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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The present investigations were made to evaluate the curative effects of pyridoxal phosphate on diabetic neuropathy. Thirty-two diabetic outpatients with neuropathy were selected for the study. Pyridoxal phosphate was administered orally 60 mg a day for 15-200 days. Twenty of the cases showed more or less an improvement of subjective and/or objective symptoms of neuropathy. The curative effects on both neuralgic pain of extremities and sensory disturbances such as paresthesia or hypesthesia were remarkable, but less amelioration was seen in autonomic dysfunctions. The mean motor nerve conduction velocity of right ulnar nerve was significantly increased from 49.9±0.8 m/sec to 52.1±O.7 m/sec after the treatment. The clinical effects of pyridoxal phosphate was remarkable especially in the cases with good diabetic control and without vascular complications. The role of vitamin B_6 in the metabolism of carbohydrate, lipid and amino acids was discussed from the point of its influence on nervous dysfunction.
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[in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
494-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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[in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
494-495
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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[in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
495-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1967 Volume 35 Issue 6 Pages
495-496
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1967 Volume 35 Issue 6 Pages
496-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
496-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1967 Volume 35 Issue 6 Pages
496-497
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
497-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
497-498
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
498-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
498-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
499-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
499-500
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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[in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
500-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
500-501
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
501-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
501-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
501-502
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
502-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
502-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1967 Volume 35 Issue 6 Pages
502-503
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
503-
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
503-504
Published: June 25, 1967
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[in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
504-505
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
505-
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
505-
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
505-506
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
506-
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
506-
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1967 Volume 35 Issue 6 Pages
506-507
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1967 Volume 35 Issue 6 Pages
507-
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
507-508
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
508-
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
508-509
Published: June 25, 1967
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[in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
509-
Published: June 25, 1967
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[in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
509-510
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
510-
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
510-
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
510-511
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
511-
Published: June 25, 1967
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1967 Volume 35 Issue 6 Pages
511-512
Published: June 25, 1967
Released on J-STAGE: February 10, 2018
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