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Mitsuo KAMIMURA, Shigeo TAKAHASHI, Shozo SATO
Article type: Article
1964 Volume 30 Issue 5 Pages
341-344
Published: November 25, 1964
Released on J-STAGE: February 07, 2018
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The authors considered that γ-oryzanol had the actions to promote the skin microcirculations, by the following observations. 1) Increases of the capillary resistance, 2) decreases of the capillary permeability, 3) rises of the skin temperature and 4) increases of the skin blood volumes.
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Yoshiaki MIYATA, Naomi TAKAHASHI, Eiji SUMITA, Akimitsu SAMUKAWA
Article type: Article
1964 Volume 30 Issue 5 Pages
345-349
Published: November 25, 1964
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The diets added with the single or combined supplementation of 4〜6 mg of pyridoxine hydrochloride and 20 mg of antibiotic "Achromycin" per kg were given for 3〜6 weeks to male White Leghorn chicks of seven days old, and the influences of these substances on the growth of chicks were examined. The basal diet consisted of ground corn, soybean oil meal, fish meal, minerals, liver oil and vitamin B mixture devoid of pyridoxine. During 3〜6 weeks trials, the single supplementation of 4,8,16 mg of pyridoxine hydrochloride or 20 mg of Achromycin and the combined supplementation of 4〜6 mg of pyridoxine hydrochloride with 20 mg of Achromycin improved the growth of chicks. The increase of their body weight showed 5〜10%, 5〜10% and 10% in the group which was given pyridoxine hydrochloride only, and in the group of the combined supplementation of pyridoxine hydrochloride with Achromycin, each group showed 10% over the control. The increase of body weight of chicks given the single doses of 20 mg of Achromycin is almost the same as that of 4 mg of pyridoxine hydrochloride. Vitamin B_6 contents of this basal diet were determined as 5.2 mg/kg by the microbiological method using Saccharomyces carlsbergensis. As the results, authors concluded that White Leghorn chicks require 8.5 mg of pyridoxine per kg of ration for their good growth.
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Chikataro KAWASAKI, Kozo OKADA, Sachie FUJITA, Atuko NIINOMI
Article type: Article
1964 Volume 30 Issue 5 Pages
350-353
Published: November 25, 1964
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Twenty kinds of pyrimidine compounds, structually related to thiamine were examined for the growth inhibition of the thiamine requiring microorganisms ; Lactobacillus fermenti 36 and Kloeckera apiculata IFO No. 0630. The results indicated that only pyrimidine compounds similar to the pyrimidine moiety of thiamine were inhibitory to the growth of both microorganisms. Among the compounds shown in Table 1,2-methyl-4-aminopyrimidine (No.8), 2-methyl-4-amino-5-methylpyrimidine (No.7) and other derived compounds (No.2〜6) from No.7 were inhibitory in each concentration of 10^<-4> mole/tube, while such derived compounds as 5-carboxylic or 5-methanesulfonic were proved to be non-inhibitory. Compounds No.1 and 3 were the most inhibitory ; the compound having a -CH_2N= substitution in the 5-position is more antagonistic to thiamine than that having -CH_2- in 5. Inhibition by other compounds having different substitutions in 2 or 4 position of the pyrimidine moiety was negligible ; all the compounds showed practically no inhibition.
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Chikataro KAWASAKI, Kozo OKADA, Sachie FUJITA
Article type: Article
1964 Volume 30 Issue 5 Pages
354-357
Published: November 25, 1964
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The growth inhibitory effects of 2-methyl-4-amino-5-aminomethylpyrimidine (AMPm) and some pyrimidine compounds for thiamine requiring L.fermenti and Kl. apiculata decreased with increasing amount of thiamine and were almost compensated by the presence of 1/2500 and 1/25000 equimolecular amounts of thiamine, respectively and no effects were seen for such microorganisms requiring no thiamine, as L.arabinosus 17-5,L.casei, Streptococcus faecalis and Sacch. carlsbergensis. From these results, the inhibitory effect of the pyrimidine compounds seems to be specifically antagonistic with thiamine. As shown in Fig.1 and 2,for the growth of L.fermenti AMPm inhibited the utilization of thiamine diphosphate (TDP) 3 to 6 times more than that of thiamine, while for Kl.apiculata no difference of inhibitory effect of AMPm was observed between thiamine and TDP.
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Chikataro KAWASAKI, Chisae YAMADA
Article type: Article
1964 Volume 30 Issue 5 Pages
358-363
Published: November 25, 1964
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Thiamine, when added to the thiamine-free Atkin's broth in increasing concentrations, first stimulated the growth of Saccharomyces carlsbergensis 4228 slightly better than the control, but in higher concentrations than 10^<-6>M, caused a marked growth-inhibition which was counteracted by pyridoxine. Modified thiamine compounds in place of thiamine, were tried for their effects on the growth of Saccharomyces. (a) Thiamine disulfide, thiamine propyldisulfide and other asymmetric disulfides of thiamine showed similar patterns of stimulation and inhibition on the increase of concentrations. Only O-benzoylthiamine disulfide exhibited no inhibition but growth-stimulation in higher concentrations. (b) Among the acyl derivatives of thiamine, S-benzoylthiamine was the most powerful inhibitor. The effects of others were shown according to the following inequality : Thiamine>S-benzoylthiamine>O-benzoylthiamine>S-benzoylthiamine phosphate. O, S-diacetylthiamine exhibited no inhibition. O, S-dibenzoylthiamine (DBT) stimulated the growth in high concentration. (c) Both alkoxycarbonylthiamines and thioether-type thiamines showed no inhibition. (d) Thiamine monophosphate and diphosphate exhibited similar inhibition pattern to thiamine, dihydrothiamine, one tenth the inhibition of thiamine, but thiothiamine stimulated the growth. The inhibition caused by modified thiamine compounds was counteracted by pyridoxine. Correlations between growth-stimulation to Lactobacillus fermenti and growth-inhibition to Saccharomyces carlsbergensis by the compounds were observed. Noninhibitory DBT distributed much less than thiamine and was present as such in the cells. Hence the inhibitory effect of modified thiamine compounds seems to depend upon whether they are converted to thiamine by Saccharomyces carlsbergensis.
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Nobu KATAYAMA, Soichi NIWA
Article type: Article
1964 Volume 30 Issue 5 Pages
364-370
Published: November 25, 1964
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For the purpose of improving the methods of stabilizing of vitamin C and blistering agent and preventing of food dye fading, changes of vitamin C content, CO_2 production and food dye fading after 30 days storage were studied on the various products under different conditions of humidity and wrapping materials. The conclusion obtained was as follows : (1) When aluminium foil was used as wrapping container, oxydative degeneration of vitamin C and denaturation of blistering agent and food dyes were prevented fairly well in high temperature and humidity during a long period. (2) When polyethylene laminated cellophane or damp-proof cellophane was used for wrapping material, reduction in vitamin C content and CO_2 production were 10 or 20% and about 30%, respectively, and 10 to 20% fading of food dyes was demonstrated. Furthermore when humidity reached 90%. its effect was intensified very distinctly, therefore the keeping quality of the drinks was not to be expected by these wrapping material. (3) However double or triple wrapping by polyethylene laminated cellophane assured better results in a milieu of 85% humidity and 37℃ for 30 days storage. Deteriolating effects of storage in each condition seems to be closely related to the duration of storage.
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Yoshikuni TAMAO, Tadakatsu KATO, Shoichi SHIMIZU, Saburo FUKUI
Article type: Article
1964 Volume 30 Issue 5 Pages
371-376
Published: November 25, 1964
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This paper deals with the comparison of the effects of cyanocobalamin and hydroxocobalamin on the syntheses of acid-soluble deoxyribosyl compounds and DNA in vitamin B_<12>-starved cells of Lactobacillus leichmannii grown on a medium containing deoxyguanosine instead of vitamin B_<12>. The ratio of DNA to RNA in the cells was significantly lower than that of normal cells. The cell elongation was not recognized. contrary to an usual vitamin B_<12> starvation. The incubation of the cells in a medium containing cyanocobalamin, hydroxocobalamin or DBCC permitted a remarkable increase of acid-soluble deoxyribosyl compounds followed by DNA synthesis without the increase of RNA content as well as the growth. Hydroxocobalamin exerted a greater effect than cyanocobalamin. DBCC exogenously added showed almost the same activity as that of hydroxocobalamin being inconsistent with the authors' observation that vitamin B_<12>-group compound derived from external cobalamins in the cells seems to be identical with DBCC or a closely related substance. The reason might be attributed to splitting of the deoxyadenosyl ligand from DBCC molecule when it is uptaken into the bacterial cells.
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Makoto MINO, Harumi KASAHARA, Yoshinori SATO
Article type: Article
1964 Volume 30 Issue 5 Pages
377-380
Published: November 25, 1964
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The specificity of various saturated fatty acids, capric, lauric, myristic, palmitic and stearic acids for the esterification of vitamin A by pancreas, liver and intestine was studied. By acetone powder of pancreas, the ester formation of vitamin A was demonstrated with all fatty acids, except capric acid and specificity between fatty acids was not recognized. By acetone powder of liver no significant esterification was observed with all fatty acids. By acetone powder of intestine the esterifying enzyme was not specific toward myristic, palmitic and stearic acids, but with capric and lauric acids the esterification ability was small.
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Tokio KOBAYASHI, Ryohei TAKATA
Article type: Article
1964 Volume 30 Issue 5 Pages
381-384
Published: November 25, 1964
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Several experiments were done for the purpose of preparing the dried yeast containing more than 1% of thiamine and following culture condition was chosen. Saccharomyces sake was cultured under aeration for ten hours at 27℃ in a medium consisting of rice embryo extract supplemented with 80mg per litre of thiamine hydrochloride. Dried yeast was obtained in a yield of 3g per litre and its thiamine content was 1.67%.
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Michio UCHIDA
Article type: Article
1964 Volume 30 Issue 5 Pages
385-390
Published: November 25, 1964
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This study aimed at elucidation of the fate of vitamin B_<12> taken up by the liver following intravenous administration together with intrinsic factor as originally described by Okuda. Rats were given ^<60>Co-labeled vitamin B_<12> mixed with a purified hog intrinsic factor preparation, in quantities sufficient to bind all the vitamin, and the radioactivity in the liver was followed for three weeks. It was found that the liver radioactivity gradually decreased with concomitant redistribution of radioactivity to other organs, and that the fecal excretion of radioactivity markedly increased by the intrinsic factor. Subsequent Studies demonstrated that choleresis increased the fecal excretion and that radioactivity appeared in bile promptly and in considerable quantities following intravenous administration of vitamin B_<12> and intrinsic factor. The possibility of an extraintestinal role of intrinsic factor and the site of vitamin B_<12> uptake inside the liver are discussed.
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Akira KOTAKI, Kunio YAGI
Article type: Article
1964 Volume 30 Issue 5 Pages
391-398
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1964 Volume 30 Issue 5 Pages
399-
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
399-
Published: November 25, 1964
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[in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
399-400
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1964 Volume 30 Issue 5 Pages
400-
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
400-
Published: November 25, 1964
Released on J-STAGE: February 07, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
401-
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
401-
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
401-
Published: November 25, 1964
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[in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
402-
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
402-403
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
403-
Published: November 25, 1964
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[in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
403-404
Published: November 25, 1964
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[in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
404-405
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
405-406
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1964 Volume 30 Issue 5 Pages
406-407
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
407-
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
407-408
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
408-
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
408-
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
408-409
Published: November 25, 1964
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
409-
Published: November 25, 1964
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[in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
409-410
Published: November 25, 1964
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[in Japanese], [in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
410-
Published: November 25, 1964
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[in Japanese]
Article type: Article
1964 Volume 30 Issue 5 Pages
411-
Published: November 25, 1964
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