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Volume 70, Issue 3
Displaying 1-17 of 17 articles from this issue
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Kenji SODAArticle type: Article
1996 Volume 70 Issue 3 Pages 103-113
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
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Tetsuo TARAYAArticle type: Article
1996 Volume 70 Issue 3 Pages 115-128
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSRoles of the components of the nucleotide loop of adenosylcobalamin (AdoCbl) in catalysis were studied with diol dehydrase using synthetic analogs of AdoCbl. Neither the α-D-ribofuranose ring nor the functional groups of the ribose moiety were essential for coenzymic function. Therefore, the D-ribose moiety seems important as a spacer to keep 5,6-dimethylbenzimidazole (DBI) in the proper position. Coenzyme analogs in which the D-ribose and DBI moieties of the coenzyme were replaced by a trimethylene and imidazole or pyridine, respectively, induced suicidal inactivation of enzyme .Adenosylcobinamide methyl phosphate behaved as a pseudocoenzyme, although adenosylcobinamide neither functioned as coenzyme nor bound tightly to apoenzyme. Therefore, it is evident that the phosphodiester moiety of the nucleotide loop is essential for tight binding to apoenzyme and that the DBI moiety of AdoCbl plays a pivotal role in protecting the highly reactive radical intermediates from unfruitful side reactions. Evidence is presented to show that cobalamin is bound to the enzyme with DBI coordinated to the cobalt atom. It is also concluded that the nucleotide loop-modified analogs are useful probes for exploring the cobalamin binding sites of proteins.View full abstractDownload PDF (1094K) -
Sumie SATOWA, Haruyo EBINUMA, Tuyako OKIDOArticle type: Article
1996 Volume 70 Issue 3 Pages 129-138
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSPyridoxal 5'-phosphate (PLP) is a coenzyme of δ-aminolevulinic acid synthetase, and is involved in hemoglobin (Hb) synthesis. We investigated the serum and erythrocyte PLP and pyridoxal (PL) status in 81 young women with iron deficiency anemia in which Hb is lower than 11 g/dl by measuring HPLC. In these young women, although the serum PLP level was significantly lower than that in normal young women, the erythrocyte PLP and PL levels were significantly high. The erythrocyte PLP and PL levels correlate negatively with Fe, Hb and hematocrit. After one month of chalybeat treatment, the young women who showed improvement in anemia had decreased erythrocyte PLP and PL levels, while they increased serum PLP level. In the young women who showed no improvement in anemia, erythrocyte PLP and PL levels were higher than improvement group, while plasma PLP and PL levels were lower, both before and after treatment. Our results showed that in subjects with iron deficiency anemia, PLP and PL were retained in the erythrocytes.View full abstractDownload PDF (918K) -
Article type: Appendix
1996 Volume 70 Issue 3 Pages 139-144
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
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[in Japanese]Article type: Article
1996 Volume 70 Issue 3 Pages 145-
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 3 Pages 145-146
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 3 Pages 146-147
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
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[in Japanese]Article type: Article
1996 Volume 70 Issue 3 Pages 147-148
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (339K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1996 Volume 70 Issue 3 Pages 148-149
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
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[in Japanese]Article type: Article
1996 Volume 70 Issue 3 Pages 149-
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (181K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1996 Volume 70 Issue 3 Pages 149-150
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
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[in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 3 Pages 151-152
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
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[in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 3 Pages 152-153
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
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[in Japanese]Article type: Article
1996 Volume 70 Issue 3 Pages 153-
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
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[in Japanese]Article type: Article
1996 Volume 70 Issue 3 Pages 155-156
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
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[in Japanese]Article type: Article
1996 Volume 70 Issue 3 Pages 156-158
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (325K) -
[in Japanese], [in Japanese]Article type: Article
1996 Volume 70 Issue 3 Pages 158-159
Published: March 25, 1996
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (266K)
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