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Volume 85, Issue 3
Displaying 1-35 of 35 articles from this issue
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Kaori Yasuda, Keiko Hayashi, Shinichi Ikushiro, Masaki Kamakura, Eiji ...Article type: Article
2011 Volume 85 Issue 3 Pages 109-114
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSOur previous studies revealed that the double mutant of CYP105A1 from Streptomyces griseolus, R73V/R84A, has a high ability to convert vitamin D_3(VD_3) to its biologically active form, 1α,25-dihydroxyvitamin D_3(1α,25(OH)_2D_3), suggesting the possibility of practical application of R73V/R84A to produce 1α,25(OH)_2D_3. Thus, we examined the expression of R73V/R84A in Streptomyces lividans TK23. As expected, the metabolites 25(OH)D_3 and 1α,25(OH)_2D_3 were detected in the recombinant S. lividans cell culture. In addition, novel polar metabolites 1α,25(R),26(OH)_3D_3 and 1α,25(S),26(OH)_3D_3, both of which were known to have a high antiproliferative activity and a low calcemic activity, were observed at a ratio of 5:1. The crystal structure of the double mutant with 1α,25(OH)_2D_3 and a conformational search of 1α,25(OH)_2D_3 in its active site strongly suggest that a novel hydrogen-bond network including 1α-hydroxyl group and several water molecules plays an important role in the substrate-binding for 26-hydroxylation. In conclusion, we demonstrated that R73V/R84A can catalyze hydroxylations at C25-, C1- and C26 (27) positions of VD_3 to produce biologically useful compounds.View full abstractDownload PDF (1015K) -
[in Japanese], [in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 115-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (179K) -
Toshiharu YagiArticle type: Article
2011 Volume 85 Issue 3 Pages 116-126
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSNatural vitamin B_6 consists of six compounds which are pyridoxine, pyridoxal, pyridoxamine, pyridoxine 5'-phosphate, pyridoxal 5'-phosphate, and pyridoxamine 5'-phosphate. They show the same nutritional efficiency. Plant-derived foods contain pyridoxine-β-glucoside, a storage form of vitamin B_6. These compounds have chemically distinct functional groups. Many methods have been developed to determine them. Current application status and characteristics of microbiological methods, spectroscopic, fluorometric, electrochemical, and capillary electrophoretic methods, and liquid chromatographic, including HPLC, methods are commented. Advantages and disadvantages of several sample preparation methods for analysis of vitamin B_6 compounds are also described. Finally, a principle and protocols of a challenging method, all enzymatic HPLC method, which is applicable for determination of the individual vitamin B_6 compounds and pyridoxine-β-glucoside, is described.View full abstractDownload PDF (1482K) -
Masakazu MiuraArticle type: Article
2011 Volume 85 Issue 3 Pages 127-130
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSRecently, the measurement of folic acid by automated immunoassay analyzer system was performed for the routine clinical laboratory tests in patients with pernicious anemia and other diseases. Measurement of serum folic acid has been reported to be an easy and rapid assay and highly precision for routine tests using the automated immunoassay analyzer system. For these immunoassays, it becomes the problem in the mean folic acid concentration in serum by the difference of the standard samples and a difference of the measurement methods. On the other hand, it indicates that the standardization measurement may provide a useful tool to measure folic acid concentration if the folic acid measurement by LC/MS or LC/MS/MS will spread in its routine method.View full abstractDownload PDF (590K) -
Tomono Uchida, Saiko IkedaArticle type: Article
2011 Volume 85 Issue 3 Pages 131-133
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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Takashi Hayakawa, Kohei YamamotoArticle type: Article
2011 Volume 85 Issue 3 Pages 134-137
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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Kazuhiro YamadaArticle type: Article
2011 Volume 85 Issue 3 Pages 138-140
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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Hideyuki HayashiArticle type: Article
2011 Volume 85 Issue 3 Pages 141-145
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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Keiko Tazuya-Murayama, Motohiro Mishima, Kazuko YamadaArticle type: Article
2011 Volume 85 Issue 3 Pages 146-148
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2011 Volume 85 Issue 3 Pages 149-150
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (393K) -
[in Japanese], [in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 150-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (225K) -
[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 150-151
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (383K) -
[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 151-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (211K) -
[in Japanese], [in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 151-152
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2011 Volume 85 Issue 3 Pages 152-153
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 153-154
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 154-155
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 155-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 155-156
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (411K) -
[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 156-157
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (414K) -
[in Japanese], [in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 157-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (239K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2011 Volume 85 Issue 3 Pages 157-158
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (391K) -
[in Japanese], [in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 159-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (187K) -
[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 160-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (227K) -
[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 160-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (227K) -
[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 161-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 161-162
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 162-163
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 163-164
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
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[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 164-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (239K) -
[in Japanese]Article type: Article
2011 Volume 85 Issue 3 Pages 164-165
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (291K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2011 Volume 85 Issue 3 Pages 166-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (219K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2011 Volume 85 Issue 3 Pages 166-167
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (338K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2011 Volume 85 Issue 3 Pages 168-169
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (381K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
2011 Volume 85 Issue 3 Pages 169-
Published: March 25, 2011
Released on J-STAGE: October 10, 2017
JOURNAL FREE ACCESSDownload PDF (225K)
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