VITAMINS

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Print ISSN : 0006-386X

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Volume 47, Issue 6

Displaying 1-50 of 78 articles from this issue

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Biosynthesis of Ascorbic Acid Glucoside

Yukio SUZUKI, Toshio MIYAKE, Kei UCHIDA, Aiko MINO

Article type: Article
1973 Volume 47 Issue 6 Pages 259-267
Published: June 25, 1973
Released on J-STAGE: March 02, 2018

DOIhttps://doi.org/10.20632/vso.47.6_259

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It was found by paper chromatography that a new derivative of L-ascorbic acid having reducing power on both 2,6-dichlorophenol-indophenol and silver nitrate was formed in the incubation mixture containing maltose, sodium L-ascorbate, thiourea and a partially purified α-glucosidase from Aspergillus niger. After purification by precipitation with lead acetate and by successive separation chromatographically on paper and on Sephadex column, the new derivative was isolated as powdered preparation and assumed to be ascorbic acid glucoside from various data, viz., R_F values, absorption spectra at acidic, neutral and alkaline pH, products by hydrolysis with a highly purified α-glucosidase from Aspergillus niger, and absorption spectra of reaction products with both 2,4-dinitrophenylhydrazine and diazotized p-nitroaniline. D-Araboascorbic acid also appeared to act as glucosyl acceptor, but was less efficient. The activity of the formation of ascorbic acid glucoside was observed in the molds belonging to Aspergillus and Penicillium, but not to Rhizopus.

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Some Considerations on the Determination of Ascorbie Acid in the Plant Tissues containing Anthocyan

Atsuko BABA

Article type: Article
1973 Volume 47 Issue 6 Pages 269-277
Published: June 25, 1973
Released on J-STAGE: March 02, 2018

DOIhttps://doi.org/10.20632/vso.47.6_269

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Determination of ascorbic acid (AsA) in the plant tissues containing anthocyan (Antc) was examined experimentally in the following points : 1) In 4 kinds of vegetables containing Antc, as compared with their green portions containing no Antc, total AsA values by 2,4-dinitrophenylhydrazine method agreed with the values estimated by the thin layer chromatography method (Fujita et al.^<6)>). 2) Japanese acid clay decolourized the colour of the sample solution with the partial oxidation of AsA to dehydroascorbic acid, but without influence to the total AsA value. The efficient adsorption of anthocyanins was observed by columns of cation-exchange resins such as Dowex 50 W × 4 and less by Dowex 50 W × 8 without oxidation of AsA.

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Effects of Tetrahydrofolic Acid on Liver of CCl_4-injured Rat

Tetsuya SHIOZAKI

Article type: Article
1973 Volume 47 Issue 6 Pages 279-284
Published: June 25, 1973
Released on J-STAGE: March 02, 2018

DOIhttps://doi.org/10.20632/vso.47.6_279

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Severe hepatic damage was induced by CCl_4 inhalation in rats treated with phenobarbital. The curative effects of tetrahydrofolic acid (THFA) vs. folic acid (FA) were examined with hematological picture, urinary excretion of formiminoglutamic acid (FIGLU), liver histology and dihydrofolic acid (DHFA) reductase activity as indicators. CCl_4-inhalation resulted in the increase of neutrocyte and the decrease of lymphocyte. The activity of hepatic DHFA reductase was greatly reduced with the increase of urinary output of FIGLU. Urinary excretion of FIGLU returned to normal on treatment with THFA 5 mg/kg s. c. but not with FA.

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The Study on Group-specific Protease for Pyridoxal Enzymes in Rat Skeletal Muscle

Kunio SUZUKI

Article type: Article
1973 Volume 47 Issue 6 Pages 285-295
Published: June 25, 1973
Released on J-STAGE: March 02, 2018

DOIhttps://doi.org/10.20632/vso.47.6_285

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The existance of a new group specific protease for pyridoxal enzymes was proved in skeletal muscle of rats. The enzyme was purified until homogeneous protein judging from acrylamide gel disc electrophoresis and certain properties were studied. The molecular weight of the enzyme was calculated as about 13,000 using Sephadex G-75 column chromatography with several kinds of markers. The purified enzyme shows strict substrate specificity for pyridoxal enzyme group and the enzyme inactivates only apo-form of pyridoxal enzyme group tested and holo-form of pyridoxal enzymes are not affected. No the other types of enzyme were inactivated by the purified protease. Usual synthetic substrates for trypsin like p-toluene-sulfonyl-L-arginine methyl ester are not cleaved by the protease. N-acetyl-L-tyrosine-ethyl ester which is one of substrates for chymotrypsin was cleaved but p-aminophenyl-β-phenyl-propionate which is chymotrypsin inhibitor does not show any inhibitory action. The optimum pH of the protease exists in alkaline side at 9.0,and 10^<-4>M of Ca^<2+> ion inhibits this enzyme activity till 50%.

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[title in Japanese]

[in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 297-298
Published: June 25, 1973
Released on J-STAGE: March 02, 2018

DOIhttps://doi.org/10.20632/vso.47.6_297

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[title in Japanese]

[in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 298-299
Published: June 25, 1973
Released on J-STAGE: March 02, 2018

DOIhttps://doi.org/10.20632/vso.47.6_298

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[title in Japanese]

[in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 299-300
Published: June 25, 1973
Released on J-STAGE: March 02, 2018

DOIhttps://doi.org/10.20632/vso.47.6_299

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 300-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_300_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 300-301
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_300_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 301-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_301_1

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 301-302
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_301_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 302-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_302_1

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 302-303
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_302_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 303-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_303_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 303-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_303_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 303-304
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_303_3

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 304-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_304_1

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[title in Japanese]

[in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 304-305
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_304_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 305-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_305_1

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[title in Japanese]

[in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 305-306
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_305_2

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 306-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_306_1

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 306-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_306_2

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 307-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_307_1

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 307-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_307_2

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 308-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_308_1

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 308-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_308_2

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Article type: Article
1973 Volume 47 Issue 6 Pages 308-309
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_308_3

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 309-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_309_1

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Article type: Article
1973 Volume 47 Issue 6 Pages 309-310
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_309_2

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 310-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_310_1

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 310-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_310_2

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Article type: Article
1973 Volume 47 Issue 6 Pages 310-311
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_310_3

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[title in Japanese]

[in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 311-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_311

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 312-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_312_1

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 312-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_312_2

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Article type: Article
1973 Volume 47 Issue 6 Pages 312-313
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_312_3

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Article type: Article
1973 Volume 47 Issue 6 Pages 313-
Published: June 25, 1973
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DOIhttps://doi.org/10.20632/vso.47.6_313_1

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 313-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_313_2

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Article type: Article
1973 Volume 47 Issue 6 Pages 314-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_314_1

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 314-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_314_2

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Article type: Article
1973 Volume 47 Issue 6 Pages 314-315
Published: June 25, 1973
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DOIhttps://doi.org/10.20632/vso.47.6_314_3

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Article type: Article
1973 Volume 47 Issue 6 Pages 315-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_315_1

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[title in Japanese]

[in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 315-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_315_2

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 315-316
Published: June 25, 1973
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DOIhttps://doi.org/10.20632/vso.47.6_315_3

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 316-317
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_316

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 317-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_317_1

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 317-318
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_317_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1973 Volume 47 Issue 6 Pages 318-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_318_1

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 318-319
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_318_2

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[title in Japanese]

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Article type: Article
1973 Volume 47 Issue 6 Pages 319-
Published: June 25, 1973
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.47.6_319_1

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