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Akinori AKAMATSU, Ya Wun WHAN, Kazuhiko YAMADA, Norimasa HOSOYA
Article type: Article
1986 Volume 60 Issue 5-6 Pages
199-204
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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The effects of high environmental temperature and exercise on the ascorbic acid metabolism in rats were studied. The heating and exercise gave stressful effects on the animals, i.e., after the exposure to six hours of heating or 30 minutes of forced swimming exercise for four consecutive days, the wet weights of spleen and thymus were reduced, while the serum adrenocorticoid levels increased compared with the control group. The concentration of ascorbic acid in serum, thymus, and spleen significantly decreased in both heat-treated and exercised groups. There was a negative correlation between ascorbic acid and adrenocorticoid levels in the adrenals. The L-gulonolactone oxidase activity increased significantly in the liver microsomal fraction in both groups, accompanied by an increase in the V_<max> but without any changes in the K_m. These results suggest that the consumption of ascorbic acid in the rats was increased by the stressful conditions of heating and exercise, which provoked the increased biosynthesis of the vitamin.
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Tadashi DOI, Tetsunori KAWATA, Akio MAEKAWA
Article type: Article
1986 Volume 60 Issue 5-6 Pages
205-209
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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In order to confirm vitamin B_<12> deficiency, hepatic methylmalonyl CoA mutase (MMCoA mutase) and tetrahydropteroylglutamate methyltransferase (Met synthase) activities were examined in the vitamin B_<12> deprived rats, fed on a soy bean protein diet for 150 days. Vitamin B_<12> deprivation increased urinary contents of methylmalonic acid with the prolongation of experimental period. The concentration of vitamin B_<12> in the brain, heart, liver, spleen, kidneys and testes decreased remarkably when rats were fed on vitamin B_<12>-deprived diet. The decrease of vitamin B_<12> concentration by vitamin B_<12> deprivation was most remarkable in the kidneys among the tissues and organs tested. The results of determination of vitamin B_<12> dependent enzymes activities showed that vitamin B_<12>-deprived rats had an activity of hepatic MMCoA mutase 1/20 of that of vitamin B_<12>-supplemented rats. Moreover, hepatic Met synthase activity in the vitamin B_<12>-deprived rats decreased to 1/20 of that in the vitamin B_<12>-supplemented control.
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Fumio SHIMURA, Sunja KWON, Norimasa HOSOYA
Article type: Article
1986 Volume 60 Issue 5-6 Pages
211-217
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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A new method was developed to isolate quantities of vitamin D-dependent calcium-binding protein (CaBP) from the avian intestine. In contrast to previous method, the necessity of producing rachitic animals was excluded in the present method. The intestinal CaBP level was considerably high in hens (>18 months old) treated with vitamin D_3 and 1,25-dihydroxyvitamin D_3 together. CaBP was purified from the intestinal supernatant of the vitamin D_3/1,25-dihydroxyvitamin D_3-treated hens by a convenient two step procedure. Following partial purification by ammonium sulfate fractionation, CaBP was adsorbed by DEAE-Sephadex A-25 ion exchanger in the presence of EDTA and then selectively eluted from the resin with CaCl_2. This simple procedure provided a 126-fold purification of CaBP over the original supernatant. From the intestine of ten animals 33mg of purified CaBP was obtained with the yield of 42% of the initial amount.
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Akira KANDA, Shigeto IKEDA, Fumio SHIMURA, Norimasa HOSOYA
Article type: Article
1986 Volume 60 Issue 5-6 Pages
219-224
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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Pyridoxal 5'-phosphate inhibited the binding to DNA-cellulose of the 1,25-dihydroxyvitamin D_3 receptor extracted from hen intestinal chromatin. Pyridoxal and pyridoxamine 5'-phosphate did not show this potency. The inhibition by pyridoxal 5'-phosphate of the binding of the receptor to DNA-cellulose was reversibly restored by isonicotinic acid hydrazide, which can function as a pyridoxal 5'-phosphate scavenger through formation of pyridoxal hydrazone. The inhibitory action of pyridoxal 5'-phosphate, however, changed into an irreversible fashion after reduction of the pyridoxal 5'-phosphate-treated chromatin extract. These results suggest that the pyridoxal 5'-phosphate action on the 1,25-dihydroxyvitamin D_3 receptor involves formation of Schiff base between this active vitamin B_6 and ε-amino group of lysine. Also participation of the lysyl residue to the DNA-binding of the receptor is discussed.
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
225-
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
225-226
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
226-
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
226-227
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1986 Volume 60 Issue 5-6 Pages
227-
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
227-228
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1986 Volume 60 Issue 5-6 Pages
228-229
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
JOURNAL
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
229-
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
JOURNAL
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
231-
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
231-233
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
233-235
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
235-236
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
236-238
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
238-239
Published: June 15, 1986
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
239-240
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
240-241
Published: June 15, 1986
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
241-
Published: June 15, 1986
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
243-244
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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[in Japanese], [in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
244-245
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
245-246
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
246-248
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
248-249
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
JOURNAL
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[in Japanese]
Article type: Article
1986 Volume 60 Issue 5-6 Pages
250-252
Published: June 15, 1986
Released on J-STAGE: March 14, 2018
JOURNAL
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