VITAMINS
Online ISSN : 2424-080X
Print ISSN : 0006-386X
Search
Advanced search
OR
Browse
Search
Volume 40, Issue 4
Displaying 1-22 of 22 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
  • STUDIES ON MYOINOSITOL : (X) EFFECT OF MYOINOSITOL ON THE PREVENTION OF FATTY LIVER INDUCED BY OROTIC ACID
    Akira KOTAKI, Misao OKUMURA, S. H. HASAN, Kunio YAGI
    Article type: Article
    1969 Volume 40 Issue 4 Pages 219-223
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_219
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Induction of fatty liver in rats by feeding on diets containing 1.5% orotic acid for 7 days, was accompanied with the increase in the lipid TBA value and with the decrease in the lipid phosphorus concentration. However, fat accumulation was prevented by injecting 30mg of myoinositol or choline subcutaneously every day during the experimental period . The prevention was found to be about 30-50% with myoinositol and 60-80% with choline. However, the effects of choline and myoinositol were not additive; the effect due to the injection of both choline and myoinositol was rather close to the effect of myoinositol alone. The increase in TBA value and the decrease in lipid phosphorus concentration were also found to be prevented by injecting these lipotropic factors.
    View full abstract
    Download PDF (923K)
  • ABSORPTION OF METHYLCOBALAMIN IN COMPARISON WITH CYANOCOBALAMIN
    Kunio OKUDA, Keisuke YASHIMA, Isao TAKARA, Tetsuro KITASAKI, Mitsuko K ...
    Article type: Article
    1969 Volume 40 Issue 4 Pages 224-231
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_224
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Methylcobalamin(CH_3-B_<12>)preparations labeled with ^<57>Co and ^<14>CH_3 were used in rats as well as in man to study its absorption in comparison with cyanocobalamin (CN-B_<12<)-^<57>Co. The preparations were more than 90% pure, and when eluted with distilled water from a carboxymethylcellulose column, a small unidentified fraction was seen preceding the major peak of CH_3-B_<12> labeled with ^<57>Co, but not with ^<14>C. Absorption of CH_3-B_<12>-^<57>Co by oral administation and in an intestinal loop in rats was quite similar to that of CN-B_<12> with respect to amount and speed at varying dose levels. The behavior toward intrinsic factor concentrate, both hog and homologous, was also the same. Intrinsic factor bound CH_3-B_<12> was also photosensitive, and ^<14>CH_3 Was readily released and dialyzed following photolysis. In man, the urinary excretion in 24 hours of ^<51>Co following oral administration of 0.5μg of CH_3-B_<12>-^<57>Co and flushing with 1 mg of CN-B_<12>-^<57>Co(Schilling test) was about 1/3 that obtained with CN-B_<12>. The liver uptake of ^<57>Co was greater with CH_3-B_<12> than with CN-B_<12> when net absorption was the same in rats, suggesting conversion of the former to either hydroxocobalamin or other coenzyme forms in tissues.
    View full abstract
    Download PDF (844K)
  • BEHAVIOR OF ORALLY ADMINISTERED METHYLCOBALAMIN IN THE RAT INTESTINE AND ABSORPION
    Kunio OKUDA, Keisuke YASHIMA, Isao TAKARA, Tetsuro KITASAKI, Kazunori ...
    Article type: Article
    1969 Volume 40 Issue 4 Pages 232-239
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_232
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Methylcobalamin labeled with ^<57>Co or ^<14>CH_3 Was used in rats to study the absorption and biochemical changes in the gut, in comparison with its photolysis prduct, the most likely degradation product. The following were the results : 1)^<14>C0_2 was expired quickly and in large quantities following oral adminstration of ^<14>CH_3-cobalamin photolyzed, but only a trace of ^<14>C0_2 in the first three hours after oral administration of non-photolyzed ^<14>CH_3-cobalamin. 2) When absorption was compared between ^<57>Co-cobalamin and its photolysis product using three diverse doses of 10,200 and 2,OOOmμg, no significant differences were demonstrated. 3) Using physiological doses, no differences were obtained in speed of absorption between methylcobalmin and its photolysis product. The mechanism for absorption involving intrinsic factor was essentially the same for both cobalamins. There were no differences in blood and tissue concentrations and urinary excretion of radioactivity between the two forms after oral administration.
    View full abstract
    Download PDF (969K)
  • INFLUENCE OF α-TOCOPHERYLNICOTINATE ON MICTOCIRCULATION IN ACROCYANOSIS
    Mitsuo KAMIMURA
    Article type: Article
    1969 Volume 40 Issue 4 Pages 240-244
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_240
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Clinical observations and cooling rewarming test were made on six persons with severe acrocyanosis in the affected parts before and after administration of tocopherylnicotinate. The experimental results indicated the normal reactions in the raised limbs, and the delayed reactions in the hanging. After tocpherylnicotinate administration, above disturbances were gone and clinical appearances were also cured. Discussing the results, it was concluded that tocopherylnicotinate had the persistent and strong accelerating effects on the microcirculation of the venous side, and these effects were brought by the function which tocopherylnicotinate did not permited increasing of permeability in venous vascular wall.
    View full abstract
    Download PDF (1075K)
  • APPLICATION OF FLUOROMETRY ON FAT SOLUBLE VITAMINS : (II) FLUORESCENT PROPERTIES OF THE TRIMETHYLHYDROQUINONE ISOPRENE DERIVATIVE STANDARDIZED FOR TOCOPHEROLS ASSAY
    Toshikazu TABATA, Hiroko YAMANOUCHI, Yasuyuki SHIMIZU
    Article type: Article
    1969 Volume 40 Issue 4 Pages 245-250
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_245
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    As was reported in the previous paper, when α-tocopherol was treated with alkaline manganese dioxide, it forms a substance having strong fluorescence by excitation with 365 mμ Hg ray, accompanied by many byproducts with faint fluorescence. In the present paper, fluorescent, properties were investigated on a mixture of synthesized trimethylhydroquinone isoprene derivatives. It was observed that this material contains dominantly a fluorescent substance closely related to the substance described above, and can be used as the standard for the fluorometric determination of tocopherols.
    View full abstract
    Download PDF (581K)
  • STUDIES ON THE NUTRITIONAL VALUE OF ALLIUM PLANTS : (L) EXPERIMENTAL STUDIES OF AFFINITY OF THIAMINE PROPYLDISULFIDE FOR BLOOD
    Koh IKEDA
    Article type: Article
    1969 Volume 40 Issue 4 Pages 251-259
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_251
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    In vivo experiments were carried out in detail on the reduction and the uptake of TPD by the blood and the following results were obtained. 1) There was a limit to the reduction and the uptake of TPD by the blood. The maximum reduction occured in the supernatant of the suspension with 0.5ml of human blood cells was about 200μg and the maximum uptake into the blood cells was about 400μg. The maximum reduction by 0.5ml of plasma was about 70μg. 2) The SH radical in the blood cells played a major role in reduction of TPD and this action was markedly suppressed by the addition of PCMB. 3) The reduction of TPD by the blood cells increased markedly by the co-existence of plasma when the blood cells were intact.
    View full abstract
    Download PDF (1053K)
  • STUDIES ON THE NUTRITIONAL VALUE OF ALLIUM PLANTS : (LI) EXPERIMENTAL STUDIES ON THE AFFINITY OF THIAMINE PROPYLDISULFIDE FOR TISSUES OF VARIOUS ORGANS
    Koh IKEDA
    Article type: Article
    1969 Volume 40 Issue 4 Pages 260-262
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_260
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The similar experiments with the blood cells were carried out with the tissues of various organs for the reduction and the uptake of TPD compared with the ordinary thiamine and the following results were obtained. TPD was taken up in a large amount to the tissues and the main factor which reduced TPD to thiamine in the organs seemed to be the SH radical as in the case of the blood cells. The action of this factor was markedly inhibited by the addition of PCMB. On the contrary to this phenomenon the amounts of thiamine taken up into the various tissues was small and hardly affected by the addition of PCMB in the case of the ordinary thiamine.
    View full abstract
    Download PDF (349K)
  • STUDIES ON THE NUTRITIONAL VALUE OF ALLIUM PLANTS : (LII) NUTRITIONAL SIGNIFICANCE OF THE INGESTION OF GARLIC (A SUPPLEMENT)
    Koh IKEDA
    Article type: Article
    1969 Volume 40 Issue 4 Pages 263-267
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_263
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    When garlic extract or allicin was injected in the rabbit intestine with thiamine simultaneously, there occured a marked rise of thiamine content in the blood of the mesenterial vein. The author blieve that the results of this experiments confirmed again the report of M. Yoshimura which revealed the fact that the nutritional significance of ingestion of garlic which was customarily used as a tonic food throughout the world from the immemorial would be explained by the synthesis of allithiamine in the body when garlic was taken orally.
    View full abstract
    Download PDF (513K)
  • A NEW METHOD FOR THE DETERMINATION OF LABELED THIAMINE OR ITS DERIVATIVES
    Koh IKEDA
    Article type: Article
    1969 Volume 40 Issue 4 Pages 268-270
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_268
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    A new method for the determination of labeled thiamine or its derivatives, in stead of the widely applied wet oxidation method was deviced. The labeled thiamine or its derivatives in the materials was purified with permutit as usual and converted to thiochrome by the addition of BrCN and NaOH, then thiochrome was extracted with butanol. The butanol extract was dried up in a dish under infrared lamp and the counts of radioisotope was measured. The merits of this new method are as follows. 1) It does not require the troublesome oxidation with perchloric acid and nitric acid. 2) It is possible to assay in a shorter time than by the wet oxidation method. 3) The margin of the error is smaller compared with the wet oxidation method. 4) The sensitivity is much higher than that of the wet oxidation method. 5) This method is available not only for the determination of ^<35>S-labeled thiamine or its derivatives but also of ^<14>C-labeled compounds.
    View full abstract
    Download PDF (333K)
  • BIOGENESIS OF RIBOFLAVIN IN GREEN LEAVES : (IV) ACTIVATION AND STABILIZATION OF RIBOFLAVIN SYNTHETHASE
    Hisateru MITSUDA, Fumio KAWAI, Haruhito TSUGE
    Article type: Article
    1969 Volume 40 Issue 4 Pages 271-275
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_271
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Riboflavin synthetase which is widely spread in plants and microorganisms catalyzes the formation of riboflavin from 6,7-dimethyl-8-ribityllumazine. In the progress of purification, this enzyme preparation is rapidly inactivated in solution. It was found that the enzyme was activated and stabilized by certain reducing agents such as Na_2S0_3 and dithiothreitol in the present work. Activation of the enzyme by the addition of both Na_2S0_3 (10mM) and dithiothrertol (10mM) for 2hr at 2℃ showed about 53% as compared with the system in which reducing agents were absent. Maximum concentration of Na2S03 and dithiothreitol for the production of riboflavin was 5〜10 mM. The enzyme solution in which Na_2S0_3 (10mM) and dithiothreitol (5mM) were added, was well preserved for ten days at 2℃, while in the absence of reducing agents, this activity was lost by half times and it was impossible to regain the lost activity by adding Na_2S0_3 and dithiothreitol before the reaction-start.
    View full abstract
    Download PDF (557K)
  • THE GROWTH-STIMULATING ACTIVITY OF THIAMINE DERIVATIVES ON THIAMINE REQUIRING MICROORGANISMS : (XX) DIACETYLTHIAMINE(2)
    Chikataro KAWASAKI, Tomio NAGAYAMA, Haruo YAMASHITA
    Article type: Article
    1969 Volume 40 Issue 4 Pages 276-279
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_276
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Although diacetylthiamine (DAT) , purified by repeated recrystalization, showed no growth-stimulating activity for L. fermenti in the broth without cysteine, it was shown to be slightly growth-stimulating in 10^<-5>-10^<-4>×1/4M but inhibitory in the higher concentration in the broth with cysteine. DAT, in the broth containing thiamine-HCI (10^<-6>×l/4M) was proved to be inhibitory to growth both in presence and absence of cysteine ; 50% inhibition index is 250 (with cysteine) or 500(without cysteine) . As both cysteine and histidine stimulate hydrolysis of DAT, increased addition of cysteine to the broth(176mg%) resulted in growth-stimulation of L, fermerti but increased addition of histidine gave no stimulation for growth, because of slow hydrolysis of DAT by histidine (Fig. 5).
    View full abstract
    Download PDF (458K)
  • ANTI-THIAMINE ACTIVITY OF ACYLTHIAMINE DERIVATIVES : (I) GROWTH-INHIBITION OF LACTOBACILLUS BY O-BENZOYLTHIAMINE AND O, S-DIBENZOYLTHIAMINE
    Tomio NAGAYAMA
    Article type: Article
    1969 Volume 40 Issue 4 Pages 280-282
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_280
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    O-Benzoylthiamine(OBT) and O, S-dibenzoylthiamine(DBT) were proved to be antagonistic to thiamine for the growth of L. fermenti in the broth containing cysteine : 50% inhibition indices were 340〜490 and 410〜640,respectively. In the absence of cysteine, 50% inhibition index of OBT was 210〜240 but that of DBT was unmeasurably higher than that in the presence of cysteine. It was concluded that DBT was only antagonistic to thiamine on its conversion to OBT by cysteine.
    View full abstract
    Download PDF (329K)
  • [title in Japanese]
    Tomio NAGAYAMA
    Article type: Article
    1969 Volume 40 Issue 4 Pages 283-288
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_283
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    O-Benzoylthiamine(OBT), when incubated with thiamine-poor L. fermenti for 20 minutes, was proved to be not only poorly absorbable by the cells but also strongly inhibitory to uptake of thiamine by the cells in the broth containing thiamine. Inhibition of thiamine-uptake on Lactobacilli at the presence of OBT was clearly shown to be competitive but thiamine-uptake on Lactobacilli, when previously incubated with OBT and washed by saline at the absence of OBT, was proved to be equal to that on fresh Lactobacilli. Thus competition between thiamine and OBT was observed in the broth, not in the cells. The growth of L. fermenti in the broth containing thiamine and OBT ; 1 : 5 was shown to be equall to that without OBT but thiamihe contents of the cells during the growth was proved to be lower than those without OBT. The growth of L. fermenti in the broth containing" thiamine and OBT; 1 : 200(or 400) was retarded as compared with that without OBT and thiamine contents of the cells especially at the lag period were significantly lower than the control.
    View full abstract
    Download PDF (626K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 4 Pages 289-
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_289_1
    JOURNAL FREE ACCESS
    Download PDF (169K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 4 Pages 289-290
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_289_2
    JOURNAL FREE ACCESS
    Download PDF (316K)
  • isomeric Thiamine-butylisulfide
    [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 4 Pages 290-
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_290_1
    JOURNAL FREE ACCESS
    Download PDF (195K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 4 Pages 290-
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_290_2
    JOURNAL FREE ACCESS
    Download PDF (195K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 4 Pages 290-291
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_290_3
    JOURNAL FREE ACCESS
    Download PDF (348K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    Article type: Article
    1969 Volume 40 Issue 4 Pages 291-
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_291_1
    JOURNAL FREE ACCESS
    Download PDF (203K)
  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 4 Pages 291-292
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_291_2
    JOURNAL FREE ACCESS
    Download PDF (353K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    Article type: Article
    1969 Volume 40 Issue 4 Pages 292-
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_292_1
    JOURNAL FREE ACCESS
    Download PDF (200K)
  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1969 Volume 40 Issue 4 Pages 292-
    Published: October 25, 1969
    Released on J-STAGE: February 22, 2018
    DOIhttps://doi.org/10.20632/vso.40.4_292_2
    JOURNAL FREE ACCESS
    Download PDF (200K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top