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Volume 52, Issue 5-6
Displaying 1-21 of 21 articles from this issue
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Itsiro NAKAGAWAArticle type: Article
1978 Volume 52 Issue 5-6 Pages 195-205
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
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Atsuko ADACHI, Reiko MATSUBARA, Miki OYABU, Tazu TAKAO, Yuko TSUJI, Ta ...Article type: Article
1978 Volume 52 Issue 5-6 Pages 207-215
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSGas-liquid chromatographic (GLC) and high-performance liquid chromatographic (HPLC) methods for the determination of vitamin D_2 and ergosterol in dried yeasts were investigated and the correlation between the corresponding two methods was discussed. The GLC method for the determination of vitamin D_2 includes alkali-saponification, isolation of unsaponifiable matters, thin-layer chromatography (TLC), trimethylsilylation and GLC, whereas ergosterol is determined by applying the trimethylsilyl ethers of the unsaponifiable matters directly to GLC. In the HPLC methods, the sample solutions obtained by similar treatment according to the GLC procedure without trimethylsilylation are applied to HPLC. All of them were confirmed to be useful for the determination of vitamin D_2 or ergosterol in commercial dried yeasts. When the content of vitamin D_2 in a sample of irradiated dried yeast and that of ergosterol in non-irradiated one were repeatedly estimated by both the GLC and HPLC methods, the correlation coefficients were 0.967 for vitamin D_2 and 0.927 for ergosterol.View full abstractDownload PDF (879K) -
Tadashi KOBAYASHI, Kumiko MIZUNO, Mitsue YASUMURA, Toshio OKANOArticle type: Article
1978 Volume 52 Issue 5-6 Pages 217-222
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSHydroxyaldoxypropyl (HAP) Sephadex column chromatographic separation of photoisomers from an irradiated mixture of 7-dehydrocholesterol (7-DHC) was investigated and the following satisfactory results were obtained. (1) When the reaction mixtrue of 7-DHC irradiated with the monochromatic light of 295 nm was applied to the HAP Sephadex column chromatography, four separatable peaks were observed on the chromatogram. The data of UV spectra, TLC, GLC and HPLC showed that each peak contained previtamin D_3,vitamin D_3,tachysterol_3 and 7-DHC according to the order of elution from the column, respectively. (2) Since the irradiation with the light of 295 nm produced little amounts of lumisterol_3,the light of 320 nm known to produce comparatively large amounts of lumisterol_3 was chosen for the irradiation. When the irradiated mixture was applied to the column chromatography, lumisterol_3 was not separated from previtamin D_3. However, since previtamin D_3 is easily isomerized to vitamin D_3 by heating, the identification of lumisterol_3 will be possible by applying the thermal isomerized mixture to the column chromatography.View full abstractDownload PDF (635K) -
Yasuo WAKABAYASHIArticle type: Article
1978 Volume 52 Issue 5-6 Pages 223-228
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSThiamin pyrophosphokinase was purified approximately 120,000-fold from crude extracts of pig brain by acid and heat treatment, ammonium sulfate fractionation, DEAE-cellulose column chromatography and affinity chromatography using TMP-agarose. The purified enzyme was homogenous on disc gel electrophoresis and the molecular weight of the enzyme was estimated to be 50,000 by gel filtration with Sephadex G-100,whereas 30,500 by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These results suggest that the pig brain thiamin pyrophosphokinase may consist of two identical polypeptide chains.View full abstractDownload PDF (740K) -
Yasuo WAKABAYASHIArticle type: Article
1978 Volume 52 Issue 5-6 Pages 229-236
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSThiamin pyrophosphokinase purified to homogeneity from pig brain showed following properties. 1) The enzyme required Mg^<2+>, Co^<2+> or Zn^<2+> as a cofactor and ATP or UTP as a cosubstrate for its activity. 2) The pH optimum was between pH 6 and 7,and the Km values for thiamin and ATP were 0.43μM and 0.78 mM, respectively. 3) The reaction was inhibited by several nucleotides among which GTP, GDP, GMP and IMP showed remarkable inhibitions by competing with ATP, and Ki values for the nucleotides were 3.8,1.5,20 and 34μM, respectively. 4) Thiamin analogues such as pyrithiamin, 2-northiamin and chloroethylthiamin were competitive inhibitors for thiamin with Ki values of 0.85,5.8 and 27μM, respectively, whereas TMP, TDP and thiamin sulfate showed noncompetitive inhibitions with Ki values of 17,0.67 and 6.0μM, respectively. 5) Neuroactive amines such as serotonin and dopamine acted as weak inhibitors of the enzyme and the inhibitions appeared to be competitive with respect to thiamin. 6) The purified enzyme was reversiblly inactivated by SH reagents such as PCMB and NEM suggesting that it is an SH enzyme.View full abstractDownload PDF (830K) -
[in Japanese], [in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 237-
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
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[in Japanese], DelucaM. Luigi, [in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 237-
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
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[in Japanese], [in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 237-238
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 238-
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 238-239
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
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[in Japanese], [in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 239-
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 241-
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1978 Volume 52 Issue 5-6 Pages 241-242
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1978 Volume 52 Issue 5-6 Pages 242-
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSDownload PDF (192K) -
[in Japanese], [in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 242-243
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSDownload PDF (337K) -
[in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 243-
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSDownload PDF (198K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 243-244
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSDownload PDF (300K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 244-
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSDownload PDF (153K) -
[in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 245-246
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSDownload PDF (291K) -
[in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 246-247
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSDownload PDF (305K) -
[in Japanese]Article type: Article
1978 Volume 52 Issue 5-6 Pages 247-248
Published: June 25, 1978
Released on J-STAGE: March 07, 2018
JOURNAL FREE ACCESSDownload PDF (297K)
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