VITAMINS
Online ISSN : 2424-080X
Print ISSN : 0006-386X
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Volume 48, Issue 9
Displaying 1-20 of 20 articles from this issue
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  • Flavooxygenases
    Shozo YAMAMOTO
    Article type: Article
    1974 Volume 48 Issue 9 Pages 419-432
    Published: September 25, 1974
    Released on J-STAGE: March 02, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_419
    JOURNAL FREE ACCESS
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    Three pseudomonad monooxygenases, salicylate hydroxylase, imidazoleacetate hydroxylase and lysine oxygenase, were purified and identified to be flavoproteins. Their prosthetic flavin was flavin adenine dinucleotide. Since neither iron nor copper was detected in a significant quantity, the flavin seemed to be a sole prosthetic group of these enzymes. These and other flavooxygenases comprise a major new group of flavoproteins. Lysine oxygenase catalyzed an oxygenative decarboxylation of lysine producing an acid amide. Under anaerobic conditions, however, the enzyme showed a dehydrogenation of lysine and produced a corresponding α-keto acid. Upon the modification of the enzyme protein by sulfhydryl blocking reagents, the enzyme now catalyzed an oxidative deamination of lysine producing an α-keto acid and hydrogen peroxide. Furthermore, certain analogues such as ornithine and γ-methyllysine underwent an oxidative deamination. α-Monoamino acids were also oxidized, but only when alkylamines were present. Thus, an oxidase activity was demonstrated by the modification of either the enzyme or the substrate. The significance of this oxidase activity is discussed in terms of the oxygen activation by the flavin.
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  • The Effect of Thiamine-related Compounds on the Growth of Saccharomyces carlsbergensis strain 4228
    Tadayoshi TANAKA, Kotaro MORINAGA, Shoichi MASUSHIGE, Akio MAEKAWA, Ta ...
    Article type: Article
    1974 Volume 48 Issue 9 Pages 433-437
    Published: September 25, 1974
    Released on J-STAGE: March 02, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_433
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    The growth of Saccharomyces carlsbergensis was studied when thiamine in the vitamin B_5 assay basal medium, was replaced by various thiamine-related compounds. The results were as follows ; 1) No difference was found in the growth in the medium containing thiamine diphosphate, thiamine palmitate, thiamine isovalerate, thiamine propyldisulfide, thiamine disulfide, or the mixture of 2-methyl-4-amino-5-aminomethylpyrimidine and 4-methyl-5(β-hydroxyethyl) thiazole, as compared with thiamine respectively. But the yeast growth in the medium containing O, S-dicarbethoxythiamine was lower than that in the medium containing thiamine, while, in the medium which contained ethylthiamine or chlorothiamine instead of thiamine, the state of the growth showed a high blank. 2) Ethylthiamine or chlorothiamine had no effect on the growth of thiamine-requiring Lactobacillus viridescens.
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  • Behavior of Fat-Soluble Vitamins in Gel Filtration using Hydroxypropylated Sephadex G-50
    Fumio UEDA, Tadashi HIGASHI, Yoshikazu AYUKAWA
    Article type: Article
    1974 Volume 48 Issue 9 Pages 439-445
    Published: September 25, 1974
    Released on J-STAGE: March 02, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_439
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    After estimating the swelling properties and solvent regain. values of hydroxypropylated Sephadex G-50 (organophilic dertran gel), behavior of fat-soluble vitamins on the column was examined. The available fractionation range for gel filtration utilizing hydroxypropylated Sephadex G-50 as a bed and chloroform as an eluting solvent was found to be between 200 and 10,000 in the molecular weight, but the results on the separation of fat-soluble vitamins were not better than the previous reports (Vitamins, 39,176 (1969), 40,309 (1969)) using Sephadex LH-20. Therefore, the method would be available only for separation of compounds having large differences in their molecular size.
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  • Studies on the Binding Proteins of Vitamin D_2-sulfate
    Shunsuke KAMISHIMA, Tadao HASEGAWA, Takao SUZUKI, Shigeru INABA
    Article type: Article
    1974 Volume 48 Issue 9 Pages 447-452
    Published: September 25, 1974
    Released on J-STAGE: March 02, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_447
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    ^3H-Vitamin D_2 (^3H-D_2) and ^3H-vitamin D_2-sulfate (^3H-D_2-sulfate) were prepared by Wilzbach method and D_2-sulfate binding proteins from the rabbit intestinal mucosa and plasm were investigated in vitro. The intestinal mucosa proteins reacted with ^3H-D_2 or ^3H-D_2-sulfate, on its gel filtration using Sephadex G-200 column (1.6×70cm), were separated into six protein peaks respectively. Peak 1 equivalent to the void volume was that bound with ^3H-D_2 or ^3H-D_2-sulfate. After pronase treatment of the intestinal mucosa proteins, their binding ability with ^3H-D_2-sulfate were decreased markedly. The disk get electrophoresis of ^3H-D_2-sulfate binding proteins in the peak 1 fraction was also carried out ; all the three bands on the gel stained with amide black showed radioactivity. These results suggest that D_2-sulfate can bind with intestinal mucosa proteins to be absorbed from the intestine. When the rabbit plasm binding proteins with ^3H-D_2-sulfate were separated by gel filtration using Sephadex G-200 column, six protein peaks were observed ; the protein in peak 3 fraction was shown to be that bound with either ^3H-D_2 or ^3H-D_2-sulfate respectively.
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  • Some Properties of a Mutant Strain of Escherichia coli Requiring Lipoic Acid
    Kimitoshi NIGOTA
    Article type: Article
    1974 Volume 48 Issue 9 Pages 453-462
    Published: September 25, 1974
    Released on J-STAGE: March 02, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_453
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    A mutant strain of Escherichia coli, O8-442,requiring lipoic acid (LiA) for both aerobic and anaerobic growth in a minimal medium with glucose as a sole carbon source was isolated. The requirment for LiA could be replaceable by either acetate+succinate or acetate+lysine+methionine under aerobic conditions, but anaerobically it grew on either acetate or succinate alone. Pyruvate markedly stimu1ated.the respiration of the mutant cells grown aerobically on LiA and the addition of LiA did not cause a further stimulation. Conversely, results obtained with the cells grown aerobically on acetate+ lysine+methionine was quite different ; the degree of the pyruvate-oxidizing activity was very low but the activity was fully restored by the addition of LiA. In the same system, lipoamide (LiANH_2) was found to have no activity for the respiration of the mutant cells, while it was more active than LiA when extracts prepared from the mutant cells were used. These results suggest that the de novo synthesis of LiA is blocked in the mutant strain. A permeability barrier appears to be involved in the utilization of LiANH_2.
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 463-
    Published: September 25, 1974
    Released on J-STAGE: March 14, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_463_1
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  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 463-
    Published: September 25, 1974
    Released on J-STAGE: March 14, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_463_2
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  • [title in Japanese]
    Upendra Prasad C, M.Bose S
    Article type: Article
    1974 Volume 48 Issue 9 Pages 463-464
    Published: September 25, 1974
    Released on J-STAGE: March 14, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_463_3
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  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 464-
    Published: September 25, 1974
    Released on J-STAGE: March 14, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_464_1
    JOURNAL FREE ACCESS
    Download PDF (190K)
  • [title in Japanese]
    [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 464-
    Published: September 25, 1974
    Released on J-STAGE: March 14, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_464_2
    JOURNAL FREE ACCESS
    Download PDF (190K)
  • [title in Japanese]
    V.Khandwekar P, Nath N, C.Nath M
    Article type: Article
    1974 Volume 48 Issue 9 Pages 464-465
    Published: September 25, 1974
    Released on J-STAGE: March 14, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_464_3
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    Migasena Panata, I.Thurnham D, Pongpaew Praneet, Hongthong Kanjana, Ha ...
    Article type: Article
    1974 Volume 48 Issue 9 Pages 465-
    Published: September 25, 1974
    Released on J-STAGE: March 14, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_465_1
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 465-
    Published: September 25, 1974
    Released on J-STAGE: March 14, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_465_2
    JOURNAL FREE ACCESS
    Download PDF (206K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 465-
    Published: September 25, 1974
    Released on J-STAGE: March 14, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_465_3
    JOURNAL FREE ACCESS
    Download PDF (206K)
  • [title in Japanese]
    [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 465-466
    Published: September 25, 1974
    Released on J-STAGE: March 14, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_465_4
    JOURNAL FREE ACCESS
    Download PDF (229K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 467-
    Published: September 25, 1974
    Released on J-STAGE: March 02, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_467
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 468-
    Published: September 25, 1974
    Released on J-STAGE: March 02, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_468_1
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 468-469
    Published: September 25, 1974
    Released on J-STAGE: March 02, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_468_2
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    [in Japanese], [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 469-
    Published: September 25, 1974
    Released on J-STAGE: March 02, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_469_1
    JOURNAL FREE ACCESS
    Download PDF (184K)
  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese]
    Article type: Article
    1974 Volume 48 Issue 9 Pages 469-470
    Published: September 25, 1974
    Released on J-STAGE: March 02, 2018
    DOIhttps://doi.org/10.20632/vso.48.9_469_2
    JOURNAL FREE ACCESS
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