VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 55, Issue 7

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Interaction of Thiamin and its Decomposition Products with Dihydroxy Compounds

Fumiko HAYAKAWA, Kiku MURATA

Article type: Article
1981 Volume 55 Issue 7 Pages 293-303
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_293

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It has been known that caffeic acid and other o-diphenol compounds accelerated the decomposition of thiamin at pH 7.0〜7.5 in vitro. The composition of various thiamin analogues with caffeic acid (A), and of decomposition products of thiamin or thiamin analogues with caffeic acid or catechol (B) were investigated to clarify the mechanism of these reactions. Reaction conditions were as follows: (A): Reaction mixtures contained 3 ml of 1/15M phosphate buffer (pH 7.4), 1 ml of 30 μM thiamin analogues and 1 ml of 30 μM caffeic acid. These mixtures were incubated at 48℃ for 2 hours. (B): Reaction mixtures contained 1.0g thiamin and 0.2 g catechol in a total volume of 10ml. These mixtures were incubated at pH 7.0〜7.4 48℃ for 10 days. Results were as follows: 1. With caffeic acid or catechol: Thiamin, hydroxymethylthiamin, hydroxyethylthiamin, and oxythiamin were decomposed, but the sodium salt of thiol type thiamin and thiamin disulfide (TDS) were not decomposed. 2. These decompositions were inhibited by cysteine or ascorbic acid ratio of more than 10 molar ratio to thiamin. 3. Products of these decompositions were TDS, 2-methyl-4-amino-5-hydroxymethylpyrimidine, thiazol, several unknown products (1, 2, 3, 4 and 5) and thiazolone, thiothiazolone and diazepine (less than 3% of total decomposition products). 4. The unknown products of 2 and 3 were diminished gradually. Then water and methanol insoluble material increased gradually and reached almost 90% of the thiamin after the reaction mixture had stood for 10 days at 48℃.

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Assay Methods of Ubiquinone by High Performance Liquid Chromatography

Goichiro KATSUI

Article type: Article
1981 Volume 55 Issue 7 Pages 305-312
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_305

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[title in Japanese]

[in Japanese], Jack R. Cooper

Article type: Article
1981 Volume 55 Issue 7 Pages 313-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_313_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 313-314
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_313_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 314-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_314_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 314-315
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_314_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 315-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_315_1

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[title in Japanese]

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Article type: Article
1981 Volume 55 Issue 7 Pages 315-316
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_315_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 316-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_316

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 317-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_317_1

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[title in Japanese]

[in Japanese], Jack R. Cooper

Article type: Article
1981 Volume 55 Issue 7 Pages 317-318
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_317_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 318-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_318_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 318-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_318_2

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[title in Japanese]

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Article type: Article
1981 Volume 55 Issue 7 Pages 318-319
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_318_3

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 319-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_319

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 320-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_320_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 320-321
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_320_2

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[title in Japanese]

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Article type: Article
1981 Volume 55 Issue 7 Pages 321-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_321_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 321-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_321_2

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[title in Japanese]

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Article type: Article
1981 Volume 55 Issue 7 Pages 321-322
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_321_3

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 322-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_322_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 322-323
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_322_2

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[title in Japanese]

[in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 323-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_323_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 323-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_323_2

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[title in Japanese]

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Article type: Article
1981 Volume 55 Issue 7 Pages 324-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_324_1

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[title in Japanese]

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Article type: Article
1981 Volume 55 Issue 7 Pages 324-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_324_2

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[title in Japanese]

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Article type: Article
1981 Volume 55 Issue 7 Pages 324-325
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_324_3

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Article type: Article
1981 Volume 55 Issue 7 Pages 325-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_325_1

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1981 Volume 55 Issue 7 Pages 325-326
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_325_2

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Article type: Article
1981 Volume 55 Issue 7 Pages 326-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_326_1

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[title in Japanese]

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Article type: Article
1981 Volume 55 Issue 7 Pages 326-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_326_2

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Article type: Article
1981 Volume 55 Issue 7 Pages 326-327
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_326_3

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1981 Volume 55 Issue 7 Pages 327-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_327_1

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1981 Volume 55 Issue 7 Pages 327-328
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_327_2

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1981 Volume 55 Issue 7 Pages 328-329
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_328

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Article type: Article
1981 Volume 55 Issue 7 Pages 329-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_329_1

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Article type: Article
1981 Volume 55 Issue 7 Pages 329-330
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_329_2

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Article type: Article
1981 Volume 55 Issue 7 Pages 330-331
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_330

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Article type: Article
1981 Volume 55 Issue 7 Pages 331-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_331_1

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1981 Volume 55 Issue 7 Pages 331-332
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_331_2

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Article type: Article
1981 Volume 55 Issue 7 Pages 332-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_332

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1981 Volume 55 Issue 7 Pages 333-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_333_1

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Article type: Article
1981 Volume 55 Issue 7 Pages 333-334
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_333_2

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Article type: Article
1981 Volume 55 Issue 7 Pages 334-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_334_1

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Article type: Article
1981 Volume 55 Issue 7 Pages 334-335
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_334_2

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Article type: Article
1981 Volume 55 Issue 7 Pages 335-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_335

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Article type: Article
1981 Volume 55 Issue 7 Pages 336-
Published: July 25, 1981
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DOIhttps://doi.org/10.20632/vso.55.7_336_1

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[title in Japanese]

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Article type: Article
1981 Volume 55 Issue 7 Pages 336-337
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_336_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1981 Volume 55 Issue 7 Pages 337-
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_337_1

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[title in Japanese]

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Article type: Article
1981 Volume 55 Issue 7 Pages 337-338
Published: July 25, 1981
Released on J-STAGE: March 09, 2018

DOIhttps://doi.org/10.20632/vso.55.7_337_2

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