All issues
Volume 68, Issue 10
Displaying 1-17 of 17 articles from this issue
- |<
- <
- 1
- >
- >|
-
Hiroshi NISHIMURAl, Yuko KAWASAKI, Kazuto NOSAKAArticle type: Article
1994 Volume 68 Issue 10 Pages 551-564
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSThe activities of the enzymes involved in thiamin biosynthesis, thiamin transport system and thiamin-repressible acid phosphatase in Saccharomyces cerevisiae were coordinately repressed by exogenous thiamin .We identified a strain carrying a recessive constitutive mutation (thi80) with these activities in thiamin metabolism. The thi80 mutant shows markedly reduced activity of thiamin pyrophosphokinase (EC 2.7.6.2) and an increase of the intracellular thiamin pyrophosphate content of the mutant cells grown with exogenous thiamin was appreciably low compared to that of the wild-type strain. These results suggest that a negative regulatory mechanism by thiamin pyrophsphate as an effector participates in the control of thiamin metabolism .On the other hand, we have obtained two thiamin auxotrophic mutants carrying recessive mutations which lack the positive regulatory genes, THI2(PHO6) and THI3 for expression of thiamin metabolism. The THI3 gene differs in the regulation of thiamin metabolism. The THI3 gene differs in the regulation of thiamin transport from the THI2(PHO6) gene. We have further isolated plasmid clones from an S. cerevisiae genomic library which complement thiamin auxotrophy in these mutants. Complementations of the activities for thiamin metabolism in these mutants transformed by some plasmids with THI2(PHO6) and THI3 gene were also examined .We propose a working hypothesis that thiamin metabolism in S. cerevisiae is controlled by the positive factors, THI2(PHO6) and THI3, whose actions are regulated negatively by the intracellular thiamin pyrophosphate level.View full abstractDownload PDF (1482K) -
Motoko NAKAHIGASHl, Chika WAKAMIYA, Kazuyoshi SATOArticle type: Article
1994 Volume 68 Issue 10 Pages 565-568
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSVitamin B_<12> content in mild drastically decreased during fermentation by Lactobacillus helveticus B-1 occasionally used for production of a commercial yogurt. When vitamin B_<12> was added to mild prior to the fermentation, the maximal decrease occurred by addition of 10〜50 ng/ml and more than 90% of B_<12> compounds was unextractable from the fermented milk by a conventional KCN extraction method. However, the vitamin B_<12> content in fermented milk was partly recovered by treatment with pepsin alone or pepsin and ultrasonication.View full abstractDownload PDF (433K) -
Yoshinori ITO, Rikio SHINOHARA, Ryuichiro SASAKI, Seiko YGYU, Naomi MI ...Article type: Article
1994 Volume 68 Issue 10 Pages 569-578
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSThe relationship between serum levels of carotenoids, retinol or α-tocopherol and serum lipid peroxide levels in the inhabitants (334 males and 551 females ; aged from 40y to 83y) living in a Y town of the southern Hokkaido was investigated. In the residents with hypertention, serum levels of lipid peroxides and α-tocopherol were higher than in the subjects without hypertension, but those of carotenoids such as β-carotene and cryptoxanthin were lower. The systolic and diastolic blood pressures were inversely and significantly associated with serum levels of provitamin A such as β-carotene and cryptoxanthin in the healthy residents without diseases. There was a significant inverse-relation between serum levels of lipid peroxides and carotenoids or α-tocopherol, but not retinol. In the multiple regression analyses, serum levels of lipid peroxides were inversely and significatnly associated with order of α-tocopherol < zeaxanthin/lutein <α-carotene < cryptoxanthin < β-carotene < lycopene. In contrast, serum SOD activities were inversely associated with serum levels of α-tocopherol and retinol, but not with serum carotenoid levels. It seems that α-tocopherol and carotenoids such as β-carotene and cryptoxanthin may have a role of reducing lipid peroxidation through the interaction among each other.View full abstractDownload PDF (950K) -
Katsumi SHIBATAArticle type: Article
1994 Volume 68 Issue 10 Pages 579-585
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSThe nutritional and biological efficiency of tryptophan, anthranilic acid, and 3-hydroxyanthranilic acid as a substitute for nicotinic acid was investigated by using weanling rats fed with a nicotinic acid-free, tryptophan-limiting diet (basal diet ; negative control), basal diet containing 0.003% nicotinic acid (positive control), 0.1% tryptophan, 0.1% anthranilic acid, or 0.1% 3-hydroxyanthranilic acid. Of these three compounds, tryptophan and 3-hydroxyanthranilic acid had an activity for growth promotion, the increases in the concentrations of NAD and total nicotinamide, but anthranilic acid did not. The sum of the urinary excretion of nicotinamide and its metabolites was compared between the groups of nicotinic acid and tryptophan, and between the groups of nicotinic acid and 3-hydroxyanthranilic acid. As the results, tryptophan was approximately 1/6 as active as nicotinic acid in molar basis, and 3-hydroxyanthranilic acid 1/10 in molar basis.View full abstractDownload PDF (566K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 10 Pages 587-592
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (631K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 10 Pages 593-600
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (851K) -
Article type: Appendix
1994 Volume 68 Issue 10 Pages 601-603
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (239K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 10 Pages 605-
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (162K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 10 Pages 606-
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (172K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 10 Pages 606-607
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (302K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1994 Volume 68 Issue 10 Pages 607-
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (180K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 10 Pages 607-608
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (305K) -
[in Japanese], [in Japanese]Article type: Article
1994 Volume 68 Issue 10 Pages 608-609
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (310K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 10 Pages 609-610
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (217K) -
[in Japanese], [in Japanese]Article type: Article
1994 Volume 68 Issue 10 Pages 611-613
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (382K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 10 Pages 613-616
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (538K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 10 Pages 616-618
Published: October 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (298K)
- |<
- <
- 1
- >
- >|