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Volume 42 (1970)
- Issue 6 Pages 349-
- Issue 5 Pages 261-
- Issue 4 Pages 209-
- Issue 3 Pages 141-
- Issue 2 Pages 65-
- Issue 1 Pages 1-
Volume 42, Issue 6
Displaying 1-7 of 7 articles from this issue
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Isuke IMADA, Hiroshi MORIMOTOArticle type: Article
1970 Volume 42 Issue 6 Pages 349-363
Published: December 25, 1970
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSMany ubiquinone related compounds have been obtained photochemically, chemically, metabolically from ubiquinone-7 (Q-7) and isolated from natural sources. Their structures and the reaction mechanisms have been elucidated : Irradiation of Q-7 with sunlight results in the reduction of the quinone ring, the demethylation of the methoxyl group, the isomerization and oxidation of the isoprenoid side chain. The reduction of Q-7 and its hydroquinone compound with LiAlH_4 leads to the demethylation. The acetate of ubichromenol-6 (UC-6) undergoes cleavage of the chromene ring by the same reaction to give several quinones. Treatment of UC-6 with Na in ethanol results in the reduction and cleavage of the chromene ring. Metabolites of Q-7 in which the isoprenoid side chain was oxidized were isolated from the urine, adrenals and ovaries of animals dosed with Q-7; the possible metabolic pathway are discussed. Compounds having an epoxy group in the side chain were obtained from the heart muscle of the whale as well as cells of Pseudomonas sp. Some new observations are also reported on the behavior of the ubiquinone related compounds in teh NMR, mass spectra and in gas liquid chromatography. The ubiquinone related compounds were assayed to determine their ability to restore the activity of acetone-treated mitochondria in the succinate oxidase system.View full abstractDownload PDF (1075K) -
Toru ADACHIArticle type: Article
1970 Volume 42 Issue 6 Pages 364-368
Published: December 25, 1970
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSEffects of daily subcutaneous injection of antithyroid agents or thyroxin on change of the level of NAD in mouse liver after intraperitoneal injection of nicotinamide were examined. Injection of 1-methyl-2-mercaptoimidazole, an antithyroid agent, once a day for 2 weeks resulted in higher level of NAD for longer duration. Injection of thyroxin once a day for 8 days gave the contrary results; lower increase of NAD-level after injection of either nicotinamide or NiA. Effects of Some tranquilizers and central nervous system stimulants were also examined. Trifluoperazine, a tranquilizer, gave the similar results to reserpine, higher and longer increase of NAD after NiAA injection.View full abstractDownload PDF (496K) -
Tsutomu NOJIRI, Fumihiko TANAKAArticle type: Article
1970 Volume 42 Issue 6 Pages 369-372
Published: December 25, 1970
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSThe CoA determination by Kaplan and Lipmann^<1)>, is based on the fact that a bicarbonate extract of acetone-dried pigeon liver extract can acetylate sulfanilamide in the presence of ATP, CoA and acetate. The reaction is measured by noting the disappearance of sulfanilamide by the diazotization method of Bratton and Marshall. In the course of our studies on CoA determination, it was found that Cysteine had significantly reduced the diazotization of sulfanilamide, partly owing to insufficiency of sodium nitrite due to occurrence of so-called van Slyke reaction between sodium nitrite and Cysteine. The present communication also describes some improvements of the original diazotization procedures that bring about the reproducible and reliable results in CoA determination.View full abstractDownload PDF (350K) -
Tsutomu NOJIRI, Fumihiko TANAKAArticle type: Article
1970 Volume 42 Issue 6 Pages 373-379
Published: December 25, 1970
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSIt was demonstrated that phosphotransacetylase (acetyl-CoA : orthophosphate acetyltransferase, EC 2.3.1.8.) of Streptococcus faecalis R (ATCC 8043,IFO 3181) could be used for the determination of CoA, thru phosphotransacetylase reaction coupled with the arsenolytic cleavage of acetyl-CoA to acetylarsenate and CoA. The specific activity of the partialy purified enzyme preparation was found to be about three times as high as that of Escherichia coli 26 comparing with each other at the similar purification stages. In the reaction system containing cysteine oxidized CoA or glutathione-(S-S)-CoA was found to serve as substrates of the phosphotransacetylase reaction, whereas precursors of CoA such as pantetheine -4'-hosphate, pantethine-4'-phosphate, 3'-dephospho CoA and pantothenate had shown nc catalytic activity. Both ATP and ADP were found to be inhibitory to the reaction.View full abstractDownload PDF (638K) -
Hisateru MITSUDA, Yuzuru SUZUKI, Kenji NAKAJIMAArticle type: Article
1970 Volume 42 Issue 6 Pages 380-385
Published: December 25, 1970
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSAddion of non-ionic surface active agent, Tween 80,into a growth medium consisting of glucose, peptone, yeast extract and salts caused an extreme increment in riboflavin yield by Eremothecium ashbyii. Riboflavin production was fluctuated by supply with various surfactants having different ratios of hydrophilic and lipophilic groups. Constituents of the agents such as sorbose and fatty acids inhibited riboflavin formation. Lack of yeast extract from the medium resulted in no more stimulation of riboflavin synthesis by Tween 80. Conversely, when glucose lacked in the medium, the stimulatory effect depended only upon existence of peptone as an organic nutrient. Yeast extract was exchangable in the presence of Tween 80 with inositol, thiamine, biotin and purine bases. Xanthine and guanine were most available to augment riboflavin yield. Mechanisms of the effect of Tween 80 on ribofla-vinogenesis were discussed in connection with changes in nutrient-environment of the fungus.View full abstractDownload PDF (578K) -
Hisateru MITSUDA, Yuzuru SUZUKI, Kenji NAKAJIMAArticle type: Article
1970 Volume 42 Issue 6 Pages 386-389
Published: December 25, 1970
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSAddition of purine bases into a growth medium involving glucose, peptone, yeast extract, salts and Tween 80 led to a conspicuous augment of riboflavin yield with Eremothecium ashbyii IFO 0944. The maximal yields with guanine, xanthine, adenine and hypoxanthine were revealed at the concentrations of 1.50,1.70,1.75 and 2.0mM, respectively. In a complete medium consisting of glucose, asparagine, salts and vitamins (inositol, thiamine and biotin) riboflavin synthesis was stimulated by supply with guanine, xanthine or hypoxanthine, but not with adenine. Guanine and xanthine were most effective to increase riboflavin synthesis. However, when Tween 80 was present in the medium, addition of guanine or hypoxanthine caused an inhibition of riboflavin production, while addition of xanthine did not affect the production.View full abstractDownload PDF (438K) -
Shinta FUKUIArticle type: Article
1970 Volume 42 Issue 6 Pages 390-393
Published: December 25, 1970
Released on J-STAGE: February 27, 2018
JOURNAL FREE ACCESSThe body weight gain of the rat, intestinal absorption of B_<12>, and B_<12>-binding capacity of rat's stomach were studied by using ^<57>Co-cyanocobalamin after oral administration of synovial fluid with joint diseases in order to investigate the effect of synovial fluid of rheumatoid arthritis on the B_<12> metabolism in the rat. No change was found in body weight gain of rats by administration of synovial fluid. The ^<57>Co- B_<12> uptake in liver, kidney, stomach, spleen, particularly, in pituitary gland and adrenal of the rats treated with oral ingestion of synovial fluid of rheumatoid arthritis was lower than that of osteoarthritis. In the B_<12> recovery represented by fecal excretion of B_<12> was found to be higher in rheumatoid arthritis than that of osteoarthritis. The binding capacity of rat's stomach treated with the synovial fluid of rheumatoid arthritis was lower than that with osteoarthritis and control group.View full abstractDownload PDF (492K)
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