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Volume 68, Issue 9
Displaying 1-15 of 15 articles from this issue
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Hideyuki SUZUKI, Hidehiko KUMAGAIArticle type: Article
1994 Volume 68 Issue 9 Pages 485-499
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSγ-Glutamyltranspeptidase (GGT, EC 2.3.2.2) of Escherichia coli K-23 consists of one 39kDa large subunit and one 20kDa small subunit. The amino acid sequence of E. coli GGT has rather high similarity to eukaryotic GGTs. E. coli utilizes a γ-glutamyl amino acid as an amino acid source, for which GGT is essential. Without GGT activity E. coli cannot utilize extra-cellular glutathione as cysteine source nor glycine source. GGT was recovered from the culture broth of periplasmic leaky mutant harboring the multicopy plasmid with ggt gene and isolated simply by ammonium sulfate fractionation and Chromatofocusing column chromatography. Several γ-glutamyl amino acids were synthesized by E. coli GGT. Crystallographic analysis and site-directed mutagenesis of E. coli GGT were also performed.View full abstractDownload PDF (1837K) -
Haruki SENOO, Ryu-ichiro HATAArticle type: Article
1994 Volume 68 Issue 9 Pages 501-513
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSStellate cells (vitamin A-storing cells, lipocytes, fat-storing cells, Ito cells) exist in the perisinusoidal space of the hepatic lobule, and store 80% of retinoids in the whole body as retinyl palmitate in lipid droplets in the cytoplasm. Under physiological conditions, these cells play key roles in the control of retinoid homeostasis ; they express specific receptors for retinol-binding protein (RBP), a binding protein specific for retinol, on their cell surface, and take up the complex of retinol and RBP by receptor-mediated endocytosis. Whereas, under pathological conditions such as liver cirrhosis, these cells lose retinoids, and synthesize a large amount of extracellular matrix (ECM) components including collagen, proteoglycan and adhesive glycoproteins. Morphology of these cells also changes from the star-shaped stellate cells to that of fibroblasts or myofibroblasts. ECM components regulate the morphology, proliferation, and functions of the stellate cells. L-ascorbic acid 2-phosphate, a long-acting vitamin C derivative, further modulates this cellular regulation by ECM components.View full abstractDownload PDF (3274K) -
Kyusa ABE, Ayako KAGAArticle type: Article
1994 Volume 68 Issue 9 Pages 515-521
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDietary intake of niacin in 101 healthy elderly men and 183 women aged from 60 to 79 years was surveyed. The subjects were inhabiting in three farm villages, four mountain villages and three fishing villages in Iwate Prefecture. The intakes of niacin from food were calculated from 3-day fool records using "the Standard Tables of Food Composition" in Japan. The Intakes of tryptophan from food were calculated using "The Amino Acid Composition of Foods" in Japan. The convention is to consider 60mg of tryptophan as equivalent to 1 mg niacin. No significant differences of dietary intake of niacin were observed among the distinct areas. The mean intakes of niacin per day were 29.4±6.7 mg for men and 24.7±6.4 mg for women, respectively. These values for men and women respectively were 209% and 223% of the Recommended Dietary Allowance for the Japanese.View full abstractDownload PDF (636K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 9 Pages 523-526
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (412K) -
[in Japanese], [in Japanese]Article type: Article
1994 Volume 68 Issue 9 Pages 527-534
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (731K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 9 Pages 535-536
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (303K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 9 Pages 536-537
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (317K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1994 Volume 68 Issue 9 Pages 537-538
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (314K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1994 Volume 68 Issue 9 Pages 538-539
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (275K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1994 Volume 68 Issue 9 Pages 539-
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (138K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1994 Volume 68 Issue 9 Pages 540-
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (135K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 9 Pages 541-542
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (285K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 9 Pages 542-543
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL FREE ACCESSDownload PDF (298K) -
[in Japanese]Article type: Article
1994 Volume 68 Issue 9 Pages 543-545
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (430K) -
[in Japanese], [in Japanese]Article type: Article
1994 Volume 68 Issue 9 Pages 545-547
Published: September 25, 1994
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (378K)
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