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Volume 47, Issue 12
Displaying 1-16 of 16 articles from this issue
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Fumio UEDAArticle type: Article
1973 Volume 47 Issue 12 Pages 529-551
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSIn order to devise a new and universal method for determination of fat-soluble vitamins, studies on separation of fat-soluble vitamins by gel filtration were carried out and the following results were obtained. 1. It was found that gel filtration utilizing Sephadex LH-20 as the bed and chloroform as the eluting solvent were useful for separation of fat-soluble vitamins. 2. The values of distribution coefficient K_D and K_av for about 30 kinds of fat-soluble vitamins and related compounds were determined. It was observed that a linear correlation between molecular weight and logarithm of K_D was present and the compounds containing hydroxyl or carboxyl groups were retarded on elution to the corresponding compounds having approximately similar molecular weights. 3. In order to get information on the conditions for separation of fat-soluble vitamins by gel filtration using Sephadex LH-20 column in chloroform, experiments were made. Sample volume applied to the column for complete separation of the two solutes was determined. The relationship between resolution and column size was investigated by calculation of the value of peak resolution R. The influence of flow rate on the efficiency of a column was studied by the comparison of the value of height equivalent to a theoretical plate. 4. On the basis of the results obtained by the above fundamental experiments, gel filtration was applied to separation and determination of fat-soluble vitamins. It became evident that gel filtration was useful for determinations of vitamins A and E, of vitamin A in foods, of vitamin D in the presence of vitamins A and E, separations of tocopherols, of antioxidants and purification of vitamin A.View full abstractDownload PDF (1672K) -
Masuo MURAKAMI, Masayoshi ARIGA, Norishige SAISHOArticle type: Article
1973 Volume 47 Issue 12 Pages 553-557
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSTetrahydrofolic acid (THFA) is oxidized easily by air to produce dihydrofolic acid, folic acid, p-aminobenzoylglutamate (PABG) and some others. Separation and determination of THFA were examined. THFA was separated with a liquid chromatograph using a column of DEAE-cellulose from other oxidized products. The fractionated THFA was treated with K_2Cr_2O_7 in acidic solution and resulted to produce an equivalent amount of PABG. The produced PABG was color-developed according to conventional method and then the amount of THFA was determined photometrically at 550 nm. PABG of "The Japanese Pharmacopoeia Standard" was used as standard.View full abstractDownload PDF (492K) -
Teruo SAITOArticle type: Article
1973 Volume 47 Issue 12 Pages 559-566
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSIn the previous paper, the author reported that 2', 3'-di-o-nicotinoyl riboflavin-4'(5')-phosphate (DNRP), one of the riboflavin derivatives, decreased myocardial contractile force, arterial blood pressure, and heart rate. In the present study, the effects of DNRP on the insulin-ouabain induced arrhythmias and on antiarrhythmic actions of quinidine, ajmaline, and propranolol were investigated. Dogs anesthetized with pentobarbital sodium were used. The following are the results obtained. 1. An intravenous infusion of DNRP (0.5 mg/min, total dose=21.0〜22.5 mg/kg) did not improve the insulin-ouabain induced arrhythmias. 2. DNRP (7.5 mg/kg, i.v.) enhanced the antiarrhythmic effect of ajmaline against the insulin-ouabain induced arrhythmias. 3. DNRP (10 mg/kg, i.v.) enhanced the antiarrhythmic effect of quinidine against the insulin-ouabain induced arrhythmias. 4. DNRP (10 mg/kg, i.v.) decreased the antiarrhythmic effect of propranolol against the insulin-ouabain induced arrhythmias.View full abstractDownload PDF (581K) -
Yukinobu UCHIYAMA, Kunio OKUDAArticle type: Article
1973 Volume 47 Issue 12 Pages 567-575
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSThe ultimate purpose of this series of studies is to simplify the technical procedure in the radioassay of vitamin B_12,and the current investigation concerns itself with the use of intrinsic factor (IF) coupled covalently to cyanogen bromide activated cross-linked dextran according to the method of Wide and Killander. The results obtained have been as follow : The Sephadex-IF complex prepared in this way is stable, and the time required for the separation of the bound from the free vitamin B_12 is relatively short. The assay comprises fewer steps. The whole assay can be performed in the same tube. The standard curve may be linearized by applying a logit transformation of the response variables. The method is found to be sensitive, accurate and reproducible. Thus, sera with low vitamin B_12 levels can be measured with accuracy. On the other hand, sera with vitamin B_12 levels over 1,600 pg/ml have to be diluted, because of the range of linearity of the standard curve. The incubation step requires 3 hours and it is somewhat time consuming. In a final step, the precipitating complexes have to be washed, and some loss of Sephadex-IF complexes may occur during the process of withdrawing the supernatant. Some of the potential problems and pitfalls in this type of radio-B_12 solvent assay have been discussed. Nonetheless, this method is suitable for a routine diagnostic test and can be set up in almost any laboratory since the materials are easily avalaible.View full abstractDownload PDF (723K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 577-
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (161K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 577-578
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
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[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 578-
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 578-579
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (312K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 579-580
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 580-581
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
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[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 581-582
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
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[in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 582-583
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (311K) -
[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 583-
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (170K) -
[in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 583-584
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (309K) -
[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 584-585
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
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[in Japanese], [in Japanese]Article type: Article
1973 Volume 47 Issue 12 Pages 585-
Published: December 25, 1973
Released on J-STAGE: March 02, 2018
JOURNAL FREE ACCESSDownload PDF (115K)
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