VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 29, Issue 3

Displaying 1-41 of 41 articles from this issue

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EFFECT OF AMINO ACID CONSTITUTION UPON THE THIAMINE DEFICIENCY IN RATS

Kohei HOSHINA

Article type: Article
1964 Volume 29 Issue 3 Pages 169-182
Published: March 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.3_169

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The clinical symptoms of thiamine deficiency, such as growth, change of fur, movement, paralysis of extrimities, and also several enzymatic activities of tissues were compared for rats, fed with 3 types of thiamine deficient diet, i. e., polished rice only, supplemented with lysine and threonine, and with casein. The group of rice diet showed severe signs of thiamine deficiency earlier than those of amino acid-supplemented group. The activity of xanthine oxidase, aldolase and transketolase of the tissues decreased markedly in the former, suggesting that the inadequate composition of amino acids in the diet may accelarate the fall of several enzymatic activities relating to the thiamine deficiency. These results suggest that the high incidence of thiamine deficiency among rice-eating peoples relates in some part with the inadequacy of amino acids of their diets.

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STUDIES ON INTERACTION OF THIAMINE OR ITS RELATED COMPOUNDS WITH PROTEINS : (IX) KINETIC STUDIES ON THE REACTION BETWEEN THIOL-TYPE THIAMINES AND OXIDIZED EGG ALBUMIN

Isamu UTSUMI, Kiyoshi HARADA, Keiichi KOHNO

Article type: Article
1964 Volume 29 Issue 3 Pages 183-186
Published: March 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.3_183

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In the previous paper, it was reported that the reaction of symmetrical thiamine disulfide with the thiol groups of protein proceeds according to pseudo-first order reaction of which apparent activation energy was calculated about 12 kcal per mole. In this report, the kinetics of the reaction between thiol-type thiamines and the disulfdie groups of oxidized egg albumin was investigated. The reaction of the protein-thiamine complex formation was also found to be pseudo-first order reaction in the initial stage. The apparent activation energy was found to be about 30 kcal per mole. The values of ΔF^+, ΔS^+ and ΔH^+ were calculated from the absolute reaction rate theory. From these kinetic studies, it was further confirmed that the protein-thiamine complexes were formed through covalent bonds.

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ON THE VITAMIN B_6 SYNTHESIZING BACTERIUM

Tutomu HIGASIURA

Article type: Article
1964 Volume 29 Issue 3 Pages 187-190
Published: March 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.3_187

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One strain of vitamin B_6 synthesizing bacterium, which was isolated by Suzue and Haruna from soil, was bacteriologically studied, and it is considered that it belongs either to Aerobacter aerogenes or to Klebsiella pneumoniae.

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VITAMIN B_6 ANTAGONISTS AND GROWTH OF LACTIC ACID BACTERIA : (I) GROWTH INHIBITION BY 2-METHYL-4-AMINO-5-HYDROXYMETHYLPYRIMIDINE ANALOGUES

Teijiro MIYAMOTO, Kiku MURATA

Article type: Article
1964 Volume 29 Issue 3 Pages 191-194
Published: March 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.3_191

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Growth inhibition of Leuconostoc mesenteroides P-60 and Streptococcus faecalis R by 21 analogues of 2-methyl-4-amino-5-hydroxymethylpyrimidine (OMP) was compared with that by OMP. Following analogues showed inhibition of the growth of these bacteria in different degrees : 2-methyl-4-methylamino-5-hydroxymethylpyrimidine, 2-methyl-4-amino-5-for-mylaminomethylpyrimidine, 2-methyl-4-amino-5-methylpyrimidine, 2-methyl-4-amino-5-thiomethylpyrimidine, 2-ethyl-4-amino-5-aminomethylpyrimidine, and 2-methyl-4-amino-5-nicotinylmethylpyrimidine. Among them the inhibitory effect of 2-methyl-4-methylamino-5-hydroxy methylpyrimidine was about 3 times and 19 times as active as that of OMP to Leuc. mesenteroides and Stc. faecalis, respectively. It was proved that inhibitory effect of 2-methyl-4-amino-5-thiomethylpyrimidine was not attributed to impurity of OMP which might occured during sterilization.

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VITAMIN B_6 ANTAGONISTS AND GROWTH OF LACTIC ACID BACTERIA : (II) CHARACTERISTIC PHENOMENON ON GROWTH INHIBITION OF LEUCONOSTOC MESENTEROIDES IN THE EXISTENCE OF DL-ALANINE

Teijiro MIYAMOTO, Kiku MURATA

Article type: Article
1964 Volume 29 Issue 3 Pages 195-199
Published: March 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.3_195

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The more the ratio of 2-methyl-4-amino-5-hydroxymethylpyrimidine (OMP) to pyridoxal (PAL) up to about 5,000,the more the growth inhibitory effect of OMP on Leuc. mesenteroides P-60 increased, then the growth of the bacteria started to resume. When ratio of OMP to PAL reached to 10,000-20,000,the growth of the bacteria was again restricted. The strange phenomenon could only be observed when DL-alanine or both of L-alanine and D-alanine were present in the medium of Leuc. mesenteroides regardless of vitamin B_6 existence. The characteristic phenomenon appeared also when 2-methyl-4-methylamino-5-hydroxymethylpyrimidine or isonicotinic hydrazide was added to the medium as vitamin B_6 antagonist. The above phenomenon did not occured when Streptococcus faecalis R was cultured.

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VITAMIN B_6 ANTAGONISTS AND GROWTH OF LACTIC ACID BACTERIA : (III) BEHAVIOR OF VITAMIN B_6 GROUP TO GROWTH INHIBITION BY 2-METHYL-4-AMINO-5-HYDROXYMETHYLPYRIMIDINE

Teijiro MIYAMOTO, Kiku MURATA

Article type: Article
1964 Volume 29 Issue 3 Pages 200-202
Published: March 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.3_200

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When growth inhibition test of Leuconostoc mesenteroides P-60 in medium containing DL-alanine and limited amounts of vitamin B_6 was carried out with a series of concentration of vitamin B_6 antagonist, e. g. 2-methyl-4-amino-5-hydroxymethylpyrimidine (OMP), the growth-OMP curve showed a characteristic phenomenon which had double reflections. The ratio of OMP to different types of vitamin B_6 at the first refletion was highest when pyridoxal was used, followed by pyridoxal phosphate, pyridoxamine, and pyridoxine. The order was accordant with thet of vitamin B_6 activity to Leuc. mesenteroides. Growth of Streptococcus faecalis R was inhibited by OMP in the medium containing limited amounts of vitamin B_6 and without having DL-alanine. Potency to overcome the inhibition was order of pyridoxamine> pyridoxal phosphate> pyridoxal. The order was not same as that of vitamin B_6 activity (pyridoxamine>pyridoxal>pyridoxal phosphate) to Stc. faecalis.

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SEPARATION AND DETECTION OF SOME VITAMINS IN VITAMIN PREPARATIONS BY THIN LAYER CHROMATOGRAPHY

Seiji ISHIKAWA, Goichiro KATSUI

Article type: Article
1964 Volume 29 Issue 3 Pages 203-208
Published: March 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.3_203

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Separation and detection of some vitamins in vitamin preparations by thin layer chromatography have been investigated. Vitamin A, D and E were successfully separated after alkali saponification, if necessary, using Aluminium oxid G as the chromatographic plate and chloroform as the solvent for development. In the case of separation of vitamin K compounds, Kieselgel G and chloroform were used as each purposes. Of the water-soluble vitamins, vitamin B_1,B_6,and nicotinamide were also successfully separated using Aluminum oxid G and glacial acetic acid・acetone・methanol・benzene (5 : 5 : 20 : 70) solvents system. For the separation of riboflavin, ascorbic acic and pantothenic acid, Kieselgel G was used instead of Aluminium oxid G, and for the separation of folic acid, glacial acetic acid・n-butanol・water (1 : 4 : 5) was used. After chromatographic separation, various color reagents and ultraviolet light were required to detect the different vitamins. With the method listed above vitamins in vitamin preparations can be detected in shorter time than with paper partition chromatography and others. But beause of their lower concentrations, vitamin B_<12> and biotin could not be identified by these procedures.

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OCCURRENCE OF NEW ENZYMES, FLAVIN-INACTIVATING ENZYMES

Sei TACHIBANA

Article type: Article
1964 Volume 29 Issue 3 Pages 209-210
Published: March 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.3_209

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The growth of Lactobacillus casei was not allowed to be responsive to each of such new flavin compounds as flavin cyclic diphosphate, flavinylglucoside mononucleotide and flavin cyclic 2', 5'-mononucleotide of several molds. But these compounds were converted to the active forms by the hydrolysis of H_2SO_4. Therefore, the courrence of some new flavin-inactvating enzymes were claimed to be found by the author.

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THIN LAYER CHROMATOGRAPHY OF VITAMINS : (2) THIN LAYER CHROMATOGRAPHY OF FAT-SOLUBLE VITAMINS

Goichiro KATSUI

Article type: Article
1964 Volume 29 Issue 3 Pages 211-218
Published: March 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.3_211

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Article type: Article
1964 Volume 29 Issue 3 Pages 219-220
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_219

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[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1964 Volume 29 Issue 3 Pages 221-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_221_1

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Article type: Article
1964 Volume 29 Issue 3 Pages 221-222
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_221_2

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Article type: Article
1964 Volume 29 Issue 3 Pages 222-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_222_1

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Article type: Article
1964 Volume 29 Issue 3 Pages 222-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_222_2

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1964 Volume 29 Issue 3 Pages 222-223
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_222_3

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Article type: Article
1964 Volume 29 Issue 3 Pages 223-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_223_1

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Article type: Article
1964 Volume 29 Issue 3 Pages 223-224
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_223_2

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Article type: Article
1964 Volume 29 Issue 3 Pages 224-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_224_1

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Article type: Article
1964 Volume 29 Issue 3 Pages 224-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_224_2

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1964 Volume 29 Issue 3 Pages 224-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_224_3

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Article type: Article
1964 Volume 29 Issue 3 Pages 224-225
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_224_4

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Article type: Article
1964 Volume 29 Issue 3 Pages 225-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_225_1

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Article type: Article
1964 Volume 29 Issue 3 Pages 226-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_226_1

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Article type: Article
1964 Volume 29 Issue 3 Pages 226-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_226_2

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Article type: Article
1964 Volume 29 Issue 3 Pages 226-227
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_226_3

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1964 Volume 29 Issue 3 Pages 227-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_227_1

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1964 Volume 29 Issue 3 Pages 227-228
Published: March 25, 1964
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1964 Volume 29 Issue 3 Pages 228-
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DOIhttps://doi.org/10.20632/vso.29.3_228_1

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1964 Volume 29 Issue 3 Pages 228-229
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_228_2

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1964 Volume 29 Issue 3 Pages 229-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_229_1

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Article type: Article
1964 Volume 29 Issue 3 Pages 229-230
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_229_2

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1964 Volume 29 Issue 3 Pages 230-231
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_230

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Article type: Article
1964 Volume 29 Issue 3 Pages 231-232
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_231

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Article type: Article
1964 Volume 29 Issue 3 Pages 232-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_232_1

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Article type: Article
1964 Volume 29 Issue 3 Pages 232-233
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_232_2

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Article type: Article
1964 Volume 29 Issue 3 Pages 233-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_233_1

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Article type: Article
1964 Volume 29 Issue 3 Pages 233-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_233_2

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Article type: Article
1964 Volume 29 Issue 3 Pages 234-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_234_1

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[title in Japanese]

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Article type: Article
1964 Volume 29 Issue 3 Pages 234-
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_234_2

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Article type: Article
1964 Volume 29 Issue 3 Pages 234-235
Published: March 25, 1964
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DOIhttps://doi.org/10.20632/vso.29.3_234_3

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Article type: Article
1964 Volume 29 Issue 3 Pages 235-
Published: March 25, 1964
Released on J-STAGE: February 01, 2018

DOIhttps://doi.org/10.20632/vso.29.3_235_1

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