The patient was a 26-year-old man. During the detailed work-up after the development of pneumonia, he was diagnosed as having a mucoepidermoid carcinoma of the bronchus by transbronchial biopsy and cytology. The smear demonstrated single and loosely aggregated squamous cells, intermediate cells containing a vacuole in their cytoplasm and mucus-producing glandular cells. Histological examination of the resected tissue specimens showed cancer cells spreading discontinuously to the muscle layer. Therefore, careful follow-up was considered necessary even ofter radical operation.
We report a case of pulmonary cryptococcosis. An 81-year oldman presented with dyspnea, fever, and an abnormal chest X-ray. Sputum cytology revealed the presence in abundance of oval organisms, 3-15 μm in diameter and palely stained with hematoxylin. C. neoformans was identified by multi-staining with Alcian blue-PAS. The organisms were positive for PAS, and their capsules for Alcian blue. The capsules of the small organisms (<10μm) were not clearly stained (control and Mucicarmine 65%; Alcian blue 62%). On Alcian blue-PAS multi-staining, however, the capsules of 99% of C. neoformans cells were clearly observed and readily distinguished from the coexistent Candida albicans. Thus, Alcian blue-PAS multi-staining is useful for identifying C. neoformans, and for distinguishing them from other fungi.
We report the imprint cytological features of solid cystic (SC) tumor of the pancreas. The patient was a 40-year-old woman with abdominal inflation. CT scan revealed a 12-cm mass in the middle part of the pancreas and multiple metastatic lesions in the liver. Imprint tumor cells of the pancreas formed papillary clusters and exhibited increased nucleocytoplasmic ratio. Nuclei of euchromatin were rich, and irregular in size and shape. SC cells were positive for Periodic Acid-Schiff after diastase treatment. Histological examination showed tumor cells arranged in a papillary pattern. Immunohistchemical examination demonstrated tumor cells were positive for vimentin, progesterone receptor, NSE and synaptophysin.